Global ETD Search
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Showing 1 to 20 of 27 for “"Thioesterase"”.
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UNCOVERING THE MOLECULAR BASIS FOR FATTY ACID SYNTHASE PRODUCT SPECIFICITY: STRUCTURAL AND KINETIC EVALUATION OF THIOESTERASE I AND THIOESTERASE II
… acyl chain-length selectivity of the endogenous thioesterase (TE1) domain. With the exception of lactating breast, liver and adipose tissues, FASN expression is essentially absent in normal cells since these cells obtain lipids from the diet. In lactating breast, the product distribution of FASN …
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Modification of substrate specificity of thioesterase, reductase, desaturase by MALDI-tof-MS analysis of microbial colonies
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Examination of Abnormal Dolichol Metabolism in Infantile Batten Disease Caused by Palmitoyl Protein Thioesterase-1 (PPT1) Deficiency
… a soluble lysosomal hydrolase (palmitoyl protein thioesterase-1) that removes fatty acids from lipid modified proteins in the lysosome. It has been postulated that abnormal dolichol metabolism might be involved in NCL pathogenesis because high levels of dolichol phosphate (Dol-P) and lipid linked …
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Palmitoyl-acyl Carrier Protein Thioesterase in Cotton (Gossypium hirsutum L.): Biochemical and Molecular Characterization of a Major Mechanism for the Regulation of Palmitic Acid Content
… of a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE). In embryo extracts, PATE activity was highest at the maximum rate of reserve accumulation (oil and protein). The cotton FatB mRNA transcript abundance also peaked during this developmental stage, paralleling the profiles of PATE …
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Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.
… and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The gene spans 3.6 kb with six exons and five introns. The six exons are identical in nucleotide …
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Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.
… for a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE) and an osmotin-like pathogenesis-related 5 (PR5) protein have been isolated and characterized. PATE is a class B fatty acid thioesterase with specificity for saturated long-chain fatty acids such as palmitate, and is implicated …
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Search For The Functions That Mediate Protein Palmitoylation And Depalmitoylation
… depalmitoylation throughout their lifetime, a thioesterase responsible for mediating the bulk of these depalmitoylation reactions has remained elusive. Described here is the development and implementation of genomic screens in <i>Saccharomyces cerevisiae</i> aimed at identifying gene products …
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Synthesis and Biosynthesis of Polyketide Natural Products
… to elucidate the biosynthetic details of thioesterase-catalyzed macrocyclizations in polyketide natural products. The outcome of the enzyme's activity is divided between hydrolysis and macrocyclization and limited information is currently available pertaining to its selectivity. We …
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Data Mining for Selected Genes of Agronomic Interest from the Oil Palm Genome Using a Comparative Genomics Approach
… ACP- desaturase), two FATA (oleoyl-ACP thioesterase) and four FATB (palmitoyl-ACP thioesterase) gene families will help to elucidate the gene regulatory networks involved in fatty acid composition and oil content in oil palm. A total of 61 R gene candidates with a predicted …
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A COMPARATIVE ANALYSIS OF INTRACELLULAR CHOLESTEROL ESTERIFYING ENZYMES IN MAMMALS
… to humans. Since ACAT enzymes have an intrinsic thioesterase activity, we hypothesized that by analogy with the thioesterase domain of fatty acid synthase, the active site of ACAT enzymes may comprise a catalytic triad of serine, histidine and aspartic acid residues. Results showed that in ACAT1, …
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Chemoenzymatic synthesis of an analogue of the potent antifungal mycosubtilin
… by its enzymatic cyclization using the isolated thioesterase from <em>B. subtilis</em>. Thus far, we have successfully synthesized the analogue of mycosubtilin. Future work will focus on purifying the product and testing it for antifungal and hemolytic activity.</p>
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Acyltransferase LovD as a Simvastatin Synthase: Mutational Deconvolution, Design, Substrate Scope and Immobilization
… of directed evolution (competition between thioesterase, acyltransferase and hydrolase activities, inhibition by substrate and thermal stability), is described, and new variants with fewer mutations and comparable catalytic activities are designed. Second, a rational design of native LovD is …
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Enzymology of dicarboxylic acid metabolism in Corynebacterium sp. strain 7E1C
… 7E1C possesses a constitutive acyl-CoA thioesterase active with monocarboxyl-, w-hydroxymonocarboxyl- and dicarboxyl-CoA esters. Long chain-length acyl-CoAs are the preferred substrates.5.Corynebacterium 7E1C possesses constitutive acyl-Con synthetase(s) active with monocarboxylic, …
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The Biochemical Pathway Leading to Lpa Generation Upon Blood Coagulation
… and proteomics. We identified acyl‑protein thioesterase 1 (aka. lysophospholipase A1, accession code O75608) as a novel PLA<sub>1</sub>. Addition of this recombinant PLA<sub>1</sub> significantly increased the production of <em>sn</em>‑<em>2</em>‑esterified polyunsaturated LPCs and the …
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Solid-supported synthesis of peptidoglycan fragments and their biological evaluation
… are supported by an enzymatic study using the thioesterase activity of the R39 peptidase on (R)-S2d. Because of the specific affinity for the R enantiomer of thioesters, the enantioselective synthesis of (R)-S2d is described that makes use of the coupling agent T3P. A chromatographic method is …
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Development of metabolic pathways for the biosynthesis of hydroxyacids and lactones
… product through the recombinant expression of thioesterase B (tesB). The production of 4VL was accomplished from levulinate by adding the lactonase paraoxonase I (PON1) to the P. putida hydroxyvalerate production system. Because 4VL was found to exist in a pH-dependent equilibrium with 4HV, the …
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The Role of Astrocyte Activation in Infantile Neuronal Ceroid Lipofuscinosis
… in the lysosomal enzyme, palmitoyl protein thioesterase 1: PPT1). A mouse model of INCL, the PPT1-deficient: PPT1-/-) mouse, was recently created by a targeted disruption in the CLN1 gene. The phenotype of the PPT1-/- mouse is similar to that found in human INCL patients, suggesting the …
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Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… generates these complex molecules using thioesterase (TE) domains positioned at the C-terminus of modular biosynthetic machinery. This has spurred researchers to explore the use of TEs as in vitro macrocyclization biocatalysts, enabling the chemoenzymatic synthesis of numerous …
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