Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Thin filament"”.
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Development of Color Ratio Thin Filament Pyrometry Approach for Applications in High Speed Flames
Thin filament pyrometry is a proven technique used to measure flame temperature by capturing the spectral radiance produced by the immersion of silicon carbide filaments in a hot gas environment. In this study a commercially available CMOS color camera was used, and the spectral response of each …
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Changes in the Expression of Thin Filament-Associated Proteins in Colonic Smooth Muscle from Mice During Inflammation
… contraction in colonic smooth muscle. Although, thin filament- associated proteins such as calponin, caldesmon, tropomyosin and smoothelin do not directly participate in contraction, they regulate acto-myosin interaction and thus, muscle contraction. Calponin, caldesmon and tropomyosin inhibit …
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Functional Effects of Calcium Regulation of Thin Filaments at Single Particle Resolution
… effective treatments. In the cardiac muscle, the thin filament is composed by troponin (Tn), tropomyosin (Tm), and F-actin. It provides Ca2+-dependent regulation of contraction by modulating myosin attachment and force generation in a cooperative scheme. However, this mechanism remains unclear. To …
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Discovery of New Regulatory Proteins and Mechanisms in Muscle Biology and Disease
… myopathy (NM) typically results from sarcomere thin filament dysfunction, but the molecular function of Klhl40 is not known. We found that Klhl40 binds to two proteins: (1) nebulin (Neb), a sarcomere thin filament protein that is frequently mutated in NM; and (2) leiomodin 3 (Lmod3), a novel …
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A mm-scale aeroelastic oscillation-based anemometer
The flutter of a thin filament can provide a good indication of fluid velocity at small scales. By combining a 'fishtail'-shaped filament's aeroelastic and vortex-forced flutter modes, its oscillation frequency can be confined to scale smoothly with fluid velocity. This principle has been used to …
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Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit
… near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and myosin were detected. Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas …
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A control model of muscle contraction
… systems involved are the Krebs cycle, thick and thin filament regulation, and the generation of a cross-bridge power stroke.
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Structure of Cocrystals of Tropomyosin and Troponin
… muscle is controlled by proteins found in the thin filament. Contraction is initiated when calcium binds to troponin, causing it and tropomyosin to undergo conformational changes. Tropomyosin is an $\alpha$-helical protein with two chains (284 residues each) arranged as a coiled-coil. These …
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Effects of Actin-Myosin Kinetics on the Calcium Sensitivity of Regulated Thin Filaments
Activation of thin filaments in striated muscle occurs when tropomyosin exposes myosin binding sites on actin either through calcium-troponin binding or by actin-myosin (AM) strong binding. However, the extent to which each of these binding events contributes to thin filament activation remains …
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Contribution of troponin-I to the regulation of cardiac muscle
… and assist in the localisation of hcTnI to the thin filament. This thesis also demonstrates that a region of the N-terminus of hcTnI, close to the site of phosphorylation, interacts with F-actin and that this interaction was maintained upon monophosphorylation. The interaction between F-actin …
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A FRET Investigation into Molecular Mechanisms of Cardiac Troponin Activation in Reconstituted Thin Filaments
… through a cyclic interaction between actin thin and myosin thick filaments, regulated by intracellular Ca2+ concentration. Troponin (Tn), the Ca2+-binding protein in muscle, allosterically regulates actin and myosin interactions (crossbridge formation) by facilitating the release of two …
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Orientation of the Cardiac Myosin Regulatory Light Chain Determined by Polarized Fluorescence
… arm of the<br/>myosin motor in muscle thick filaments. Phosphorylation of RLC by myosin light<br/>chain kinase (MLCK) modulates the force and speed of muscle contraction, but the mechanisms of RLC‐mediated regulation in striated muscles are less well<br/>understood than those of …
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Identification of Phosphorylase Kinase Alpha Subunit Binding Partners in Skeletal Muscle
… participating in intramolecular interactions within PhK, α is hypothesized to associate extrinsically with skeletal muscle proteins due to its peripheral location in the holoenzyme. To identify potential muscle protein interactors, the C-terminus of α , amino acid residues 1060-1237, was …
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Structure of Cross-Bridges in Vertebrate Skeletal Muscle Activated by Photolysis of Caged Ca2+
… presumed to be rnyosin heads, on the thick filament surface. Optical diffraction patterns show strong meridional spots and layer lines up to the 6th order of the 429 Å repeat, indicating preservation and resolution of periodic structures smaller than 100 Å . Following photo-release of ca2+, …
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Dynamics of confined chemical gardens and implications for submarine methane hydrates
… Firstly, we analyse the behaviour of horizontal filaments, one of the main patterns of confined chemical gardens. We model their erratic motion by considering the diffusive supply of ions to the tip, and the spreading of product as the filament advances. We show that these effects lead to an …
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Finite element modelling and analysis of composite flywheel disk including effects of filament-winding mosaic pattern
A filament-wound spinning composite disk is characterised by the mosaic-patterned configuration of the layers produced during a filament-winding process. In this structure, each helically wound layer consists of curved triangular-shaped units alternating in the radial and circumferential …
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To Phosphorylate or Not to Phosphorylate: The Role of Tropomyosin Phosphorylation in Cardiac Function and Disease
… in the calcium dependent regulation of the thin filament of the sarcomere. α-Tm is phosphorylated solely at serine 283 and phosphorylation levels are tightly regulated. Approximately 70% of total cardiac Tm phosphorylated during fetal development, decreasing to 30% during adulthood. Total Tm …