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Showing 1 to 20 of 25 for “"Thermus thermophilus"”.
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Untersuchungen zur stimulierten GTPase von EF-Tu aus Thermus thermophilus
… Arginins 80 im ribosomalen Protein L12 aus T. thermophilus zu untersuchen wurde eine L12-R80A Mutante gereinigt. Das Wildtyp L12 wurde in den Ribosomen durch die R80A Mutante ersetzt. Die Fähigkeit dieser Ribosomen die intrinsische GTPase von EF-Tu zu stimulieren wurde mittels Hydrolyse von …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
… (CRP)-family TF from the extremophile <em>Thermus thermophilus</em> HB8. Using the <em>in vitro</em> iterative selection method Restriction Endonuclease Protection, Selection and Amplification (REPSA), we identified the preferred DNA-binding motif for TTHB099, …
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Identifizierung und Charakterisierung von thermostabilen Uracil Glykosylasen von Archaeon Methanobacterium thermoautotrophicum und Bakterium Thermus thermophilus
… thermoautotrophicum DH und Bakterium Thermus thermophilus HB27. Die in vivo Rolle der charakterisierten Proteine wurde anhand der Kombination der enzymatischen Daten mit der vergleichenden Genomanalyse diskutiert.
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Explorations of Thermus thermophilus HB8 Transcription Factors TTHA1437 and TTHA1719 using the Combinatorial Selection Method REPSA
… depending on their biological function. <em>Thermus thermophilus </em>HB8 is speculated to contain 2,245 genes, of which 70 are postulated to be transcription factors. However, but for very few, little is known regarding the genes they regulate and their biological functions. The novel …
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Biochemical characterization of a-type heme-copper oxidases in escherichia coli, bacillus subtilis and thermus thermophilus
… and shown their capability of proton pumping. Thermus thermophilus cytochrome caa3 oxidase, a member of A2 subtype, was successfully expressed, purified and characterized by site-directed mutagenesis, especially on YS pair as well as around the region of glutamate. It is suggested that the …
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Discovery of a DNA-binding Consensus and Potential Genomic Regulatory Binding Sites for the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359
… such as <em>Escherichia coli</em> and <em>Thermus thermophilus,</em> remains incomplete or lacking which renders gaps in the understanding of the regulatory networks and systems biology of many organisms. Cyclic-AMP receptor protein (CRP) regulators and fumarate and nitrate reduction …
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Studies of proton pumping mechanism in BA3 type cytochrome oxidase of thermus thermophilus and CBB3 type cytochrome oxidase of vibrio cholerae
… to the crystal structure of ba3 oxidase in T. thermophilus, two highly conserved residues, D372 and H376 which are located at the interface of subunit I and II of complex, form ion pairs with the A-propionate of active-site heme a3. A set of mutations of D372 and H376 are made and examined in …
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Entschlüsselung der Genomsequenz von Escherichia blattae und komparative Bioinformatik mikrobieller Genome
… Genomsequenzen von Methanosarcina mazei Goe1 und Thermus thermophilus HB27 mit denen der nächsten Verwandten.
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REPSA Directed Assessment of Native Cleavage Resistance of DNA to Type IIS Restriction Endonucleases and Modification of REPSA for High Temperature Application
… in thermophiles (HT-REPSA). We utilized <em>Thermus thermophilus </em>(HB-8/ATCC 27634/DSM 579) as a test organism due to its amenable nature in a laboratory setting and current status as a model thermophilic organism. We used a TetR Family (TFR) transcription factor SbtR as the model protein …
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Pink discolouration in cheese
… within this group has focused on the presence of Thermus thermophilus and its potential to cause the defect, possibly through its ability to produce carotenoids. In this thesis, a wide range of approaches, including pilot-scale cheese manufacture, UHPLC-DAD-QTof mass spectrometry, shotgun …
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intron RNAs
… over interacting regions of the tRNA in a Thermus thermophilus TyrRS/tRNATyr cocrystal structure.
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Vergleichende biochemische und strukturelle Untersuchung thermophiler α-Amylasen
… AmyB-Tt aus dem extrem thermophilen Bakterium Thermus thermophilus und AmyA-Ag sowie AmyB-Ag aus dem thermoalkaliphilen Bakterium Anaerobranca gottschalkii, untersucht. Der Schwerpunkt lag dabei auf dem Enzym aus T. maritima. Die rekombinante intrazelluläre α-Amylase aus T. maritima, die ihre …
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X-Ray crystal structure of a ribosomal protein S6, S15, S18 : rRNA complex from T. thermophilus, and investigation of conformational changes in 16S rRNA fragments from B. stearothermophilus during ribonucleoprotein assembly
… of a fragment of the 30S ribosomal subunit from Thermus thermophilus containing ribosomal proteins S6, S15, S18, and a minimal rRNA binding site (T4 RNP) containing two different three-helix junctions was solved to 2.6 A. The protein S15 contains four bundled a-helices and binds the rRNA along …
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Structural Characterization of F-type and V-type Rotary ATPases by Single Particle Electron Cryomicroscpy
… Our cryo-EM structures of the V-ATPase from Thermus thermophilus reveal that the interaction between the rotary ring (rotor) and the I-subunit (stator) is surprisingly small, with only two subunits from the ring making contact with the I-subunit near the middle of the membrane. Furthermore, …
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Yeast Chorismate Mutase: Molecular Evolution of an Allosteric Enzyme
… zu erhalten, wurde auch die Chorismatmutase on Thermus thermophilus charakterisiert, nachdem das kodierende Gen kloniert war. Dieses Enzym ist ähnlich zu der strukturell einzigartigen Chorismatmutae aus Bacillus subtilis, wird aber, im Gegensatz zu letzterem durch Tyrosin in seiner Aktivität …
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Characterization of superoxide dismutase from Actinomyces
… from Actinomyces, yeast, chicken liver, and Thermus thermophilus indicated similar composition of each subunit. The second order rate constants of each Actinomyces MnSOD were measured at pH 7.8 and found to be in the range of 0.9 - 2.8 x 10⁹ M⁻¹ sec⁻¹ as compared to the rate of 1.8 x 10⁹ M⁻¹ …
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Structural and Computational Studies of RNA Polymerase
… express and purify a Group IV sigma factor from Thermus Thermophilus.</p>
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rRNA Promoters as Targets for Transcription Factors: Structural and Functional Studies of PhERI and CarD
… has not been described. The structure of the Thermus Thermophilus CarD was solved in the lab, allowing a model for the interaction between CarD and RNA polymerase to be built. I show that CarD stimulates RNA polymerase activity at rRNA promoters, but not all promoters, by directly stabilizing …
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Structural insights into the folding of cohesin and the membrane binding of bactofilin
… Here, I determine the atomic coordinates of the Thermus thermophilus bactofilin (TtBac) monomer, the lack of polarity of the filament, and the strength of the β-stacking interface by designing a polymerisation-impairing mutation that enables crystallisation and structure determination. In …
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Biochemical characterization of respiratory chain flavoproteins
… Caldivirga maquilingensis • The NDH-2 from Thermus thermophilus, now re-classified as a coenzyme A disulfide reductase (CoADR) NDH-2 catalyzes the same reaction (NADH:quinone oxidoreductase) as complex I, however it is unable to translocate protons across the membrane. Absent in mammalian …
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