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Showing 1 to 20 of 29 for “"Thermotoga"”.
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Charakterisierung thermophiler Cellulasen aus Sulfolobus solfataricus und Thermotoga maritima
… im aktiven Zentrum ausgewählt. CelA aus Thermotoga maritima und EglA aus Pyrococcus furiosus wurden bereits in früheren Arbeiten charakterisiert und weisen ebenfalls extreme Hitzestabilität auf mit einem pH-Optimum im leicht sauren Bereich bei pH 6. Durch gezielten Austausch von …
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Biochemical and structural characterization of Sirtuins from mammals and Thermotoga maritima
… and play a role in the enzyme’s activity. The thermotoga maritima Sirtuin Sir2Tm was used as a model system for structural characterization of Sirt1. The crystal structure of Sir2Tm in complex with Ex-527, a potent Sirt1 inhibitor was solved, which combined with biophysical studies offers …
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Molekulare, biochemische und strukturelle Untersuchungen an amylolytischen Enzymen von Thermotoga maritima MSB8
Das hyperthermophile Bakterium Thermotoga maritima MSB8 (Wachstumsbereich: 55°C - 90°C) ist eines der thermophilsten und ursprünglichsten aller bisher bekannten Mikroorganismen und verwertet durch einen fermentativen Stoffwechsel organische Polymere wie z.B. Stärke. An diesem Prozeß sind …
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The organization, expression, function and evolution of some essential genes from the hyperthermophilic eubacterium thermotoga maritima
The hyperthermophilic eubacterium Thermotoga maritima grows optimally at 80°C near marine geothermal locales. Phylogenetic analyses based on various molecular sequences indicate that T. maritima and other hyperthermophilic prokaryotes have very deep phylogenetic placements; i.e., that they have …
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Functional genomics analysis of carbohydrate conversion to biohydrogen by pure and mixed cultures of hyperthermophilic Thermotoga species.
The genus Thermotoga, is comprised fermentative anaerobes with optimal growth temperatures as high as 80°C. To understand the genetic and physiological diversity within this genus, the genome sequences of five Thermotoga species (T. maritima, T. neapolitana, T. sp. RQ2, T. petrophila, T. …
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Directed evolution of novel properties starting from HisF of <i>Thermotoga maritima</i> as a structural scaffold
tHisF, der Imidazol-Glyzerol-Phosphat-Synthase aus dem hyperthermophilen Organismus T. maritima mit der Struktur einer (βα)8-Barrels, wurde als Gerüst für eine direkte Evolution von Enzymen genutzt. Die thisF-Genbibliotheken wurden durch teilweises Randomisieren von Zielpositionen im thisF-Gen im …
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Präparation und röntgenkristallographische Untersuchungen an archaebakteriellen Box C/D sRNPs und einer neuartigen Glukosyltransferase aus Thermotoga maritima MSB8
… im Jahr 1998 identifizierte Enzym Amylase B aus Thermotoga maritima MSB8 wurde aufgrund seiner Fähigkeit, Stärke und deren Abbauprodukte Amylose und Amylopektin an den α-1,4-glykosidischen Bindungen zu spalten, als α-Amylase deklariert. Eine Einordnung der Amylase B in die Glykosyl-Hydrolase …
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Versuche zur Strukturaufklärung bakterieller Thiouridin Synthetasen
… aus Escherichia coli und dem Hyperthermophilen Thermotoga maritima sollte aufgeklärt werden. Dazu wurden die entsprechenden Gene in Escherichia coli kloniert und exprimiert. Es war möglich, Aufreinigungs-protokolle für die verschiedenen Proteine zu etablieren. Bei den nachfolgenden …
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Isolation and Characterization of Thermostable Alpha-Galactosidases for Application of High-Temperature Processing of Soy Molasses
… strain KM-THCJ and hyperthermophilic Thermotoga neapolitana were examined for their potential use in processing of soy oligosaccharide waste by-products. The $\alpha$-galactosidase of strain KM-THCJ has been purified to homogeneity and has a MW of ca. 176 kDa and a subunit MW of ca. 88 …
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Assignment of Enzyme Function Through Characterization of the RuBisCO and Enolase Superfamilies
… are varied. Two highly divergent enzymes from Thermotoga maritima and Enterococcus faecalis were targeted for characterization through a multi-disciplinary effort that included structural, computational, and bioinformatic analysis as well as classical enzymology. The T. maritima and E. faecalis …
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STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF THE BACTERIAL FLAGELLAR MOTOR HOOK COMPLEX AND THE MS:C-RING INTERFACE
… complex from the representative thermophile Thermotoga maritima. The FliFC:FliGN complex reported here has subsequently been reproduced in a crystal structure of the same complex from H. pylori. Comparison of the two complexes reveal that they are essentially identical, owing to the universal …
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Vergleichende biochemische und strukturelle Untersuchung thermophiler α-Amylasen
… AmyB-Tm aus dem hyperthermophilen Bakterium Thermotoga maritima, AmyB-Tt aus dem extrem thermophilen Bakterium Thermus thermophilus und AmyA-Ag sowie AmyB-Ag aus dem thermoalkaliphilen Bakterium Anaerobranca gottschalkii, untersucht. Der Schwerpunkt lag dabei auf dem Enzym aus T. maritima. …
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Enzymatic Production of Cellulosic Hydrogen by Cell-free Synthetic Pathway Biotransformation(SyPaB)
… carbohydrate-binding module (CBM) family 9 from Thermotoga maritima Xyn10A. The inactivation halftime of C. thermocellum cellobiose phosphorylase has been enhanced by three-fold at 70°C via a combination of rational design and directed evolution. The phosphorylases with improved properties would …
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Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… phosphate pathway, was cloned from a thermophile Thermotoga maritima, and heterologously expressed in Escherichia coli, purified and characterized. High-purity RpiB was obtained by heat pretreatment through its optimization in buffer choice, buffer pH, as well as temperature and duration of …
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Characterization of Mycobacterium tuberculosis Cor, a Protein Essential for Carbon Monoxide Resistance and Pathogenesis
… expression of Cor and Cor homologue from Thermotoga maritima (TM0160) in Escherichia coli rescued it from CO toxicity, suggesting a conserved function across diverse microbial species. Importantly, the virulence of the cor mutant is attenuated in a mouse model of tuberculosis. Thus, Cor is …
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Characterization of the Diverse Substrate Specificities and Biological Roles of Polyamine Biosynthetic Enzymes in Microorganisms
… Pseudomonas, Bartonella, Nitrosomonas, Thermotoga and Aquifex showed a strong preference for L-ornithine, while the enzyme from Vibrio vulnificus (VvL/ODC) had dual specificity functioning well on both L-ornithine and L-lysine. The X-ray structure of VvL/ODC was solved in the presence of …
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Pervasive degeneracy and epistasis in a protein-protein interface
… mutagenesis study of a two-component system from Thermotoga maritima that has proven amenable to structural analysis. By solving the cocrystal structure of a histidine kinase and response regulator containing interface residues from a different interacting pair, we learned the biophysical basis …
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Cell-Free Biosystems Comprised of Synthetic Enzymatic Pathways: Development of Building Blocks, Immobilization of Enzymes, Stabilization of Cascade Enzymes, and Generation of Hydrogen
… fructose 1,6-bisphosphatase (FBP) from Thermotoga maritime, phosphoglucose isomerase (PGI) from Clostridium thermocellum, triose phosphate isomerase (TIM) from Thermus thermophiles and fructose bisphosphate aldolase (ALD) from T. maritima and T. thermophilus. The recombinant proteins …
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