Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"Thermostability"”.
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Engineering nitrilases for enhanced thermostability
… 42 °C. Several variants with either enhanced thermostability or activity at alkaline pH have been discovered by random mutagenesis and directed evolution; however, these methods are slow and limited in their explorable sequence space. The aim of this project is to investigate the structural …
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EF-Tu Protein Domains:Functions and Thermostability
Univerzita Karlova v Praze Přírodovědecká fakulta Katedra genetiky a mikrobiologie Elongační faktor EF-Tu Funkce a termostabilita domén Disertační práce Hana Šanderová Specializace: Molekulární a buněčná biologie, genetika a virologie Školitel: Prof. MUDr. Jiří Jonák, DrSc. Oddělění genové exprese, …
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The upper limit of protein thermostability
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1989.
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Increasing the thermostability of a light-producing deoxyribozyme
This thesis focuses on increasing the thermostability of the light-producing deoxyribozyme Supernova, a zinc-dependent catalyst of chemiluminescent reactions. At the structural level, sequence variants were designed by extending the triple-helix domain with different combinations of C·G·G and T·A·A …
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The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
… the G. pallidus RAPc8 NHase towards improved thermostability and then to use X-ray crystallography to investigate the molecular mechanism(s) involved in the enhanced thermostability. Two randomly mutated libraries were constructed using MnCl2 mediated errorprone PCR. The PCR reaction was …
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Engineering Enhanced Cytochromes P450: Understanding Thermostability and Mechanism Through Rational Design and Directed Evolution
… use of both methods a better understanding of thermostability, catalytic mechanism and substrate specificity was gained.
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Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
… chimeric activase displayed an improved thermostability, activated Arabidopsis Rubisco and maintained Rubisco activation state at moderately high temperature in vitro. Transgenic Arabidopsis plants expressing the chimeric activase were created, allowing for further characterization.
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In silico prediction of C57BL/6 mouse T-cell epitopes: enhancing immunogenicity assessment with PREDBL6
… and >5,000 eluted ligands for H2-Kᵇ. The thermostability assessment of MHC–peptide binding evaluates the strength and duration of the interaction between the peptide and the MHC molecule under varying temperatures. Studies have shown a positive correlation between thermostability and …
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Molecular Dynamics Simulations Provide Insight into Stability of Hyperthermophilic Endoglucanases
… temperatures and is currently limited by the thermostability of endoglucanases. For this reason, it would be beneficial to discover more high-efficiency, thermostable enzymes to utilize in the hydrolytic process. In this study molecular dynamics simulations were performed on structurally …
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Computational methods for genome screening
… on the development of tools able to predict the thermostability of a protein starting from its sequence. By means of Principal Component Analysis the codon composition of a non redundant database comprising 116 prokaryotic genomes has been analyzed and it has been showed that a cross genomic …
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Understanding, Optimization, and Application of Phosphite Dehydrogenase: Advancing NAD(P)H Regeneration
… evolution, the activity, expression, and thermostability of PTDH were also improved. A 3-fold increase in heterologous expression, 2-fold improvement in kcat, and 7000-fold increase in thermostability were achieved. The best mutants from each type of improvement were characterized in …
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Expression, Purification and Molecular Characterisation of Sideroflexin Proteins
… In the absence of a functional assay, thermostability assays and size exclusion chromatography were used to show that the purified proteins were folded, and therefore amenable to further biophysical and functional analyses. Next, the effect of lipids on the stability of human …
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Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
… in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in many biomedical applications due to its specificity, strong binding, and nuclease resistance. This study is the …
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Morpho-Physiological responses of spring wheat(Triticum Aestivum L.) to heat stress
… stability index (r = 0.71**); membrane thermostability (r = 0.68**); and plant height (r = 0.53**). This study identified membrane thermostability as a trait that explained most of variations in grain yield (80.7%); followed by tillers per unit area (10.2%) and canopy temperature …
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Morpho-Physiological responses of spring wheat(Triticum Aestivum L.) to heat stress
… stability index (r = 0.71**); membrane thermostability (r = 0.68**); and plant height (r = 0.53**). This study identified membrane thermostability as a trait that explained most of variations in grain yield (80.7%); followed by tillers per unit area (10.2%) and canopy temperature …
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Evaluation of the potential functions of Avian paramyxovirus Accessory proteins
… by reverse genetics system and tested for thermostability. Our results showed that W protein does not confer thermostability to NDV. In the second part of study, we constructed plasmids expressing APMV -2, -3 and -6V proteins and tested for IFN antagonism by a dual luciferase reporter …
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STRUCTURE-FUNCTION STUDIES OF MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASES FROM GUT COMMENSAL BACTERIA
… into BtMinpp with the aim of enhancing its thermostability – an attractive characteristic for animal feed enzymes. Sites for potential disulfide bridges were identified and a one such mutant was produced. However, the engineered protein did not show a significant enhancement in …
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High temperature stress responses of Salvia splendens and Viola X wittrockiana
… heat tolerant cultivars. Greater cell membrane thermostability (CMT) and expression of a heat shock protein of low molecular weight approximately 27kD may be responsible for heat tolerance in Vista and CBP. Primary soluble sugars sucrose and raffinose found in both the salvia cultivars, and …
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