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Showing 1 to 9 of 9 for “"Thermoplasma"”.
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Characterization of ribonucleoside diphosphate reductase from Thermoplasma acidophila
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1997.
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Untersuchung der Reaktionszyklen von Chaperoninen aus Escherichia coli und Thermoplasma acidophilum mit Hilfe der Neutronenkleinwinkelstreuung
… von Chaperoninen aus Escherichia coli und Thermoplasma acidophilum mit Hilfe der Neutronenkleinwinkelstreuung Am Thermosom wurden bisher noch keine Komplexe untersucht. Hier war von großem Vorteil, daß bei SANS kein Strahlenschaden auftritt und damit Proben modifiziert und anschließend noch …
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Structural Characterization of the Transfer Ribonucleic Acids From Halobacterium Volcanii and Other Archaebacteria
… Methanomicrobium mobile, Methanosarcina barkeri, Thermoplasma acidophilum, and Sulfolobus acidocaldarius), as analyzed by two-dimensional thin layer chromatography of the ('32)P-labelled nucleotides. Second, we describe the sequences of seventeen H. volcanii tRNAs (tentative for two) as determined …
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Proteinanalyse zur Charakterisierung der Lebensformen von Stigmatella aurantiaca
… die 20S Untereinheit des Proteasoms des Archaea Thermoplasma acidophilum bestätigt. Die Verteilung der Enzymaktivität über den Sucrosedichtegradienten läßt den Schluß zu, daß S. aurantiaca hochmolekulare Proteasen beherbergt. Diese könnten für die Degradierung von Polypeptiden während des …
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Isolation and characterization of new pterins from nonmethanogenic archaebacteria
… which is different from solfapterin was found in Thermoplasma. All of the above pterins are different from any previously described pterins and thus represent new pterins in the archaebacterial kingdom. The discovery of these new pterins is important both to pterin biochemistry and to …
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Investigating the early events in proteasome assembly
… While archaeal α and β subunits from Thermoplasma acidophilum can assemble into entire 20S units in vitro, certain α subunits from divergent species have a property to self-assemble into single and double heptameric rings. In this study, we have shown that recombinant α subunits from …
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Ribonucleotide reduction and redox regulation in Archaea : surprising twists on a common theme
… of a putative TrR (Ta0984) and Tr (Ta0866) from Thermoplasma acidophilum are presented. To explore the apparent inability of taTrR to use NADPH or NADH as a reductant, we carried out a complete electrochemical characterization, which ruled out redox potential as the source of this non-reactivity. …
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Enzymatic diversity in lipoic acid modification of proteins
Escherichia coli is one of a few organisms with a well characterized and complete metabolic model for lipoic acid metabolism. In this thesis I make discoveries through analysis of the diversity of lipoic acid metabolic genes in other organisms. By examining the distribution and phylogeny of the …
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Mechanistic characterization of acetic acid resistance enzymes of Acetobacer aceti
… structure of CS from the thermoacidophile <em>Thermoplasma acidophilum</em> (TpCS) fused to a C-terminal hexahistidine tag (TpCSH6) reported here is an open structure that, when compared with several liganded TpCS structures, helps to define a complete path for active site closure. (Abstract …