Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Therapeutic proteins"”.
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Engineering therapeutic proteins for immune modulation
… would provide insight into this type of therapeutic for humans. The ectodomains of murine Fc[gamma]Rs are highly homologous and therefore it has been difficult to engineer antibody Fc regions to specifically bind only one Fc[gamma]R. To address this challenge, we engineered the human …
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Challenges in the Pharmacokinetics of Therapeutic Proteins
… structure and complicated disposition pattern of therapeutic macromolecules, their pharmacokinetic interpretation has many challenges. Two of these challenges were investigated in this dissertation: 1) the error of classical bioavailability assessment observed during subcutaneous (SC) …
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Expression, Production, and Purification of Novel Therapeutic Proteins
<p>Interest in the production of recombinant proteins consisting of collagen binding domain (CBD) fused to a bioactive material has increased due to the targeting/attachment capabilities of CBD. For example, CBD fusions can be applied to the reversing of bone density loss and the repair of the …
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Product and host engineering for low-cost manufacturing of therapeutic proteins in the yeast Komagataella phaffii
… in low- and middle-income countries (LMICs). Therapeutic proteins, which can be stored as liquids, are more amenable to distribution in LMICs than newer modalities like therapeutic RNAs that require cryogenic storage. Currently, most therapeutic proteins are manufactured in either bacterial …
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Evaluation of dynamic light scattering as a technique for the routine monitoring of therapeutic proteins on stability
Throughout the course of biotherapeutic development and manufacturing, the detection and quantification of protein aggregates and particles is critical to ensure patient safety. Size Exclusion Chromatography (SEC) is one of the most widely used techniques for the separation of proteins and allows …
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Thiol-Disulfide Exchange in Human Growth Hormone
… is a common route to covalent aggregation of therapeutic proteins and other biologics [2, 3]. Disulfide bonds participate in hydrolytic and oxidative degradation reactions that form non-native disulfide bonds and other reactive species. The mechanisms responsible for protein aggregation are …
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Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts
… expenses could be minimized or eliminated when therapeutic proteins are expressed and orally delivered via plant cells. Chloroplasts have the machinery to fold complex and biologically active eukaryotic proteins in the soluble chloroplast stromal compartment. Protein expression through …
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Silica Colloidal Crystals As Emerging Materials For The High-Throughput Sizing Of Proteins By Packed Capillary Electrophoresis
… one of the leading problems in formulation of therapeutic proteins. The effectiveness of therapeutic proteins is diminished when they exhibit heterogeneity due to aggregates and degradation by-products. Furthermore, guidelines set forth by the FDA require therapeutic proteins to have high …
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Engineering cell-based micropharmacies for in vivo protein replacement therapy
… disorders depends on the consistent delivery of therapeutic proteins to correct underlying deficiencies or modulate disease progression. However, conventional methods, such as enzyme replacement therapy (ERT) and cancer immunotherapies, often require frequent infusions, suffer from limited tissue …
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Integrating Cell-level Kinetic Modeling into the Optimization of Cancer Therapeutics
… of new promising classes of drugs such as therapeutic proteins. Due to their ability to specifically bind targets in the body they allow to modulate specific chemical reactions and ultimately to modify the functional response of the cell, such as cell growth or cell division. Targeting …
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Upstream and downstream issues in the production of recombinant vitamin K-dependent proteins in the milk of transgenic pigs
… and pathogen safety concerns for plasma-derived therapeutic proteins, such as protein C and Factor IX, have spurred the development of recombinant DNA technology for large-scale, economically feasible production of therapeutic proteins. The mammary gland is unique in its ability to synthesize and …
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Oxidation of methionine residues in protein pharmaceuticals
… manner solvent formulations that protect therapeutic proteins against oxidation.
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Studies of Protein Stability by Hydrogen Exchange-Mass Spectrometry
With the rapid growth in using proteins as therapeutics, studying the stability of therapeutic proteins has become an essential part in the manufacturing of therapeutics to ensure the quality, safety, and efficacy of a drug product. Proteins can get exposed to various stresses and undergo various …
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Development and assessment of supercharged platelets as a novel drug delivery mechanism for promoting cardiac regeneration
… provide an ideal storage compartment for therapeutic proteins. Upon activation, these granules release their contents in a targeted manner, allowing precise, sitespecific delivery. These features possessed by platelets confers upon them the necessary means to store, transport and deliver …
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The functional evaluation of a bioactive peptide, QUB-1998, discovered from the skin secretion of the frog, Pelophylax nigromaculata
… advantages of both small molecule medicines and therapeutic proteins.<br/>Through molecular cloning, the cDNA sequence encoding a new antimicrobial peptide (AMP), named QUB-1998, has been identified in the skin secretion of Pelophylax nigromaculata. The peptide was synthesised using Solid Phase …
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Streamlining and standardizing transcriptomic analysis in Amgen process development
… Hamster Ovary (CHO) system used to manufacture therapeutic proteins is paramount to efficient CHO bioprocess optimization. This understanding can be built by analyzing and synthesizing biological data; such as transcriptomic (gene expression), proteomic (protein levels), or metabolomic …
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