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Showing 1 to 20 of 35 for “"Thapsigargin"”.
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Kontraktilität des isolierten menschlichen Kardiomyozyten. Einfluß von Calcium, Isoproterenol, Stimulationsfrequenz, Stimulationspause und Thapsigargin.
… positiven PPB. Durch Blockade der SERCA mittels Thapsigargin zeigte sich an insuffizienten Myozyten jedoch ein negativer Verlauf. Unter hoher extrazellulärer Calciumkonzentration wurde eine signifikant höhere basale Ausgangsverkürzung und eine negative PPB beobachtet. Die Ergebnisse lassen sich …
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Role of ryanodine receptors in neuronal calcium signalling and growth control
… and store-depletion signal remains undefined. Thapsigargin (TG), a sarcoplasmic/endoplasmic reticulum Ca<sup>2+</sup> A TPase pump (SERCA), inhibitor induces passive depletion of the internal Ca<sup>2+</sup> stores and triggers CCE. The universality of this signal has been widely accepted and …
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Wirkung nanomolarer Konzentrationen des Mykotoxins Ochratoxin A auf Calciumhomöostase, Wachstumsverhalten und hormonelle Signaltransduktion menschlicher proximaler Tubuluszellen
… Zusätzlich steigerte OTA den Füllungszustand von Thapsigargin-empfindlichen Ca2+-Speichern und stimulierte die Aktivität der Thapsigargin-empfindlichen Ca2+-ATPase. Eine 10-minütige Inkubation mit OTA erhöhte den zellulären cAMP-Gehalt dosisabhängig. Der Proteinkinase A Inhibitor H-89 unterdrückte …
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Studies on intracellular calcium pools in a pancreatic cell line
… Ca²⁺ release appeared to be nonspecific. Thapsigargin, an inhibitor of the intracellularCa²⁺ -ATPase, was shown to inhibit Ca²⁺ accumulation into and induce Ca²⁺ release from PANC-1 microsomes. The thapsigargin-releasable Ca²⁺ pool included the 1P3- or arachidonic acid-sensitive pool. …
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Nongenomic Effects of Estrogens on Epithelial Chloride Secretion.
… seconds to minutes) potentiates carbachol- and thapsigargin-stimulated chloride secretion measured as short circuit current in voltage-clamped T84 monolayer cultures. The cholinergic agonist carbachol and the SR Ca<sup>2+</sup> ATPase inhibitor thapsigargin stimulate chloride secretion by …
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The effect of glucagon-like peptide-1 (GLP-1) receptor agonists on cell viability, ADAM10 maturation and the proteolysis of ADAM10 substrates in SH-SY5Y cells
… (as a hypoxic mimic), methylglyoxal (MG) or thapsigargin (TG) (as Endoplasmic Reticulum stressors) were co-treated with or without GLP-1 analogues and cell viability was subsequently monitored along with the expression and proteolysis of ADAM10 and its substrates. The results of the study …
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The Unfolded Protein Response Increases Production of Pro-Angiogenic Factors by Tumor Cell Lines
… human medulloblastoma line that was treated with thapsigargin, which activates the UPR by depleting Ca2+ from the ER. In addition to the expected UPR targets, we found that ER stress inducing agents led to the transcriptional induction of several pro-angiogenic factors including vascular …
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Elucidating the regulatory mechanisms of pterygium
… cells were maintained in serum-free medium. 1M thapsigargin induced a significantly greater increase in intracellular calcium level in the serum maintained group relative to serum starved cells. Pre-incubation of cells with 1M thapsigargin ablated ligand-induced calcium responses. Disruption of …
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The regulation of hypoxia-responsive gene expression by hydroxyl radicals and intracellular calcium
… Agentien darunter Tunicamycin, Brefeldin A und Thapsigargin wurden benutzt, um das ER in Stress zu versetzen. Dabei wurde gefunden, dass sowohl Tunicamycin als auch Brefeldin A die Hypoxie-induzierte HIF-1a Aktivierung aufheben. Thapsigargin, ein Hemmer der Ca2+-ATPase, hingegen führt zu einer …
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An investigation of phytotoxicity and phylogeny of Thapsia garganica
… bacterial and yeast culture systems. Although, thapsigargin and other toxins have been reported in Thapsia garganica (Smitt, et., al. 1995) andhave been used extensively as specific inhibitors of cell metabolism in experimental systems, many of these assay systems used are not suitable for local …
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Role of SLMAP in Endoplasmic Reticulum Stress and Unfolded Protein Response
… response proteins, which were sensitized to thapsigargin-induced stress, and desensitized to palmitate-induced oxidative stress. These findings suggest that normal SLMAP levels are important for proper cardiac function, and cell viability.
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Role and regulation of mitochondrial permeability transition in cell death
… CEM cells were treated with the ER-stressor thapsigargin (THG) which led to cyclophilin-D-dependent mitochondrial release of the profusion GTPase Optic Atrophy 1 (OPA1), which controls CJ integrity, and cytochrome c leading to apoptosis. Interference RNA knockdown of Bax blocked OPA1 and …
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Stress-induced transcription factors and their role in RNA disruption in ovarian cancer cells
… after treatment with doxorubicin, docetaxel, thapsigargin, and starvation for 0, 2, 16, or 24 hours. The amount of RNA disruption was then assessed by RDA 72 hours after treatment with the same stressors. Treatment with doxorubicin, docetaxel, thapsigargin, and starvation resulted in …
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Characterization of The Role of Carma3 In Endoplasmic Reticulum Stress-Induced Nf-Κb Activation
… utilized two commonly used ER stress inducers, thapsigargin and tunicamycin, to study the mechanism. We found that two caspase-recruitment domain (CARD)-containing proteins, CARMA3 and BCL10, play a crucial roles on ER stress-induced NF-κB activation by regulating IκBα kinase activity. …
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Lead Activation of a Developmentally Regulated Calcium Channel in Rat Hippocampal Nerve Terminals
… The rise in Ca<sup>2+</sup> was reduced by thapsigargin, suggesting Pb<sup>2+</sup> activates the release of Ca<sup>2+</sup> from intracellular stores, possibly an IP<sub>3</sub> sensitive store. The Ca<sup>2+</sup> release was greatest in younger animals and gradually declined during …
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Bax-Inhibitor-1-vermittelte Neuroprotektion
… vor ER-Stress durch den Ca2+-ATPase-Inhibitor Thapsigargin schützen, während Mitochondrien-assoziierter Zelltod, ausgelöst durch den Kinaseinhibitor Staurosporin nicht inhibiert werden konnte. Außerdem zeigte sich im Rahmen eines Modells zur Induktion eines Ischämie-ähnlichen Zustands durch den …
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Regulation of the unfolded protein response by GADD34 and CReP
… the mRNA levels of CReP drop during the 24-HR Thapsigargin treated stage. Two primary proteins that bind CReP were COPS5 and SNAPIN. Understanding the UPR is important because the inhibiting of GADD34 and CReP have been shown to improve many neurodegenerative diseases.</p>
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… cells. INS-1 insulinoma cells were treated with thapsigargin to induce ER stress, which can eventually lead to apoptosis. Thapsigarin-treated INS-1 cells exhibited an increase in the ratio of BCL-x(s) (pro-apoptotic) to BCL-x(L) (anti-apoptotic) but BEL prevented this shift in splicing. Splicing …
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Regulation of autophagy, endoplasmic reticulum and unfolded protein response by glucocorticoids in physiology and disease
… of Dexamethasone with Chloroquine (CLQ), Thapsigargin (TG), Rotenone (ROT) and Bortezomib (BTZ) were used to investigate the role of autophagy, endoplasmic reticulum stress (ER stress) and unfolded protein response (UPR) respectively, in acute lymphoblastic leukemia cell lines including …
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Discovery of Alternative Artemisinin Binding Sites in Plasmodium falciparum ATPase-6
… that artemisinin binds to the homologous thapsigargin binding site of PfATP6. Herein, knowledge of the previously proposed mechanisms was utilized along with numerous computational techniques to determine a more detailed and plausible MOA for artemisinin against PfATP6. This led to the …
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