Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Tetrazine"”.
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Tetrazine-Triggered Bioorthogonal Decaging Reactions for Prodrug Activation
… Diels-Alder (IEDDA) reaction between tetrazines and strained alkenes exhibits high reaction rates and selectivity and has been widely applied for the decaging of amine prodrugs. Although amines are common in small molecule drugs, decaging methods for other functional groups are …
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Stapling and Unstapling Peptides and Proteins With S-Tetrazine
… of synthetic techniques to introduce s-tetrazine into peptides and proteins. The s-tetrazine molecule is effective for restricting peptides/proteins to macrocyclic conformations (i.e. stapling). Importantly, the incorporated s-tetrazine chromophore will undergo photodisassociation upon …
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Site-specific immobilization of tetrazine-modified green fluorescent protein on trans-cyclooctene surfaces
… areas with a site-specifically modified tetrazine-protein moiety, either through ‘click chemistry’ or non-specific binding (as a control for comparison purposes). While the strain-driven reactivity of cis- vs. trans-cyclooctene have been studied in homogenous solution systems, this is the …
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Diels -Alder reactions of [60]fullerene with 1,2,4,5-tetrazines and additions to [60]fullerene-tetrazine monoadducts
… reaction between [60]fullerene and 1,2,4,5-tetrazines was studied. Under conditions of total darkness, [60]fullerene reacts with tetrazines to form bicyclic intermediates which immediately extrudes nitrogen to yield C2nu symmetric monoadducts. Thus, 3,6-diphenyl and 3,6-di-(2 …
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Interfacial bioorthogonal chemistry for biomaterials synthesis and patterning and development of catalytic method for "turning-on" the tetrazine ligation
The bioorthogonal trans-cyclooctene-tetrazine ligation has emerged into a powerful tool in the field of biomedical research. The development and the versatile applications of tetrazine ligation was made possible by the advancement of trans-cyclooctene synthesis. Based on the previous art of …
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Tetrazines and N-Rich Organic Compounds Throughout the Fields of Materials Chemistry
… different N-rich organic compounds, primarily tetrazine-based molecules, react and coordinate with different metal ions, in order to target materials with properties ranging from single-molecule magnetism, to electron conductivity, to detonation performance. In Chapter 2, the simplest N-rich …
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Development of fluorescent semi-conductor nanocrystal conjugates for in vitro and in vivo imaging applications
… highly strained cycloolefins and a serum stable tetrazine derivative into newly developed polymeric imidazole ligands (PILs) to efficiently couple biomolecules on QDs. Unlike traditional conjugation methods, tetrazine-norbornene cycloaddition benefits from the non-interacting properties of the …
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Supporting Well-Defined Platinum Group Metals in Metal-Organic Frameworks for Heterogeneous Catalysis
… 3 describes a new synthetic strategy, termed “tetrazine click-grafting”, which enables facile post-synthetic installation of phosphorus ligands onto MOFs. Lastly, Chapter 4 reports the catalysis of MOF-supported Rh phosphines prepared via tetrazine click-grafting for the hydroformylation of …
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Raman and fluorescence spectra of azabenzenes
… coworkers obtained a fluorescence spectrum of s-tetrazine from single-mode laser excitation. This spectrum was uniquely simple since the laser mode was so narrow (~0.0002 cm<sup>-1</sup>) that only 100 transitions in the Q-branch of the 6a<sup>1</sup><sub>0</sub> band of the 18,000 …
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A Pretargeted Spect Imaging Strategy Employing Technetium-99M Based on Bioorthogonal Diels-Alder Click Chemistry
… bioorthogonal Diels-Alder click reaction between tetrazine (Tz) and transcyclooctene (TCO). The objective was to optimize the excretory pathways of the <sup>99m</sup>Tc-peptide complexes for maximum tumor targeting with the <em>in vivo </em>“click” and minimum non-target uptake. The …
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Synthesis and utilization of N-acetyl muramic acid derivatives as tools for probing information about peptidoglycan structure, biogenesis and immunological response
… NAM probe. This probe capitalized on the tetrazine-trans-cyclooctene ligation reaction, currently the fastest known bioorthogonal reaction, via the synthesis and development of a minimal tetrazine NAM. I designed downstream workflows that utilized high resolution microscopy, high …
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The Development of Novel Platforms for the Imaging of Cancer by Positron Emission Tomography
… Diels-Alder (IEDDA) reaction between a 1,2,4,5-tetrazine (Tz) and a <em>trans</em>-cyclooctene (TCO) for pretargeting. By separately injecting antibody and radioligand and allowing the two to “click” <em>in vivo</em>, overall dose to the patient can be reduced nearly 45-fold. The first half of …
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Modular Nanoparticles for Selective Cell Targeting
… NPs were further layered with additional Hep, tetrazine (Tz) modified Hep, or dextran sulfate (DS). The addition of Tz handle on Hep backbone permitted easy modification of NP surface with norbornene (NB) modified motifs/ligands, including inert poly(ethylene glycol) (PEG), cell adhesive …
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Synthesis and characterisation of ligand-bridged tungsten and chromium penta- and tetra-carbonyl complexes
… or 3,6-di-2-pyridyl-l,2,4,5-tetrazine (DPT) have been synthesised. They were characterised using IR, UV-Vis and NMR, MS, TLC and microanalysis. Electronic spectral studies showed that both the dinuclear, [4(CO)CrDPTCr(CO)4] and mononuclear, [4(CO)CrDPT] complexes have MLCT …
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Bioorthogonal Reagents: Design, Synthesis, and Reactivity
… azide–alkyne cycloaddtion (SPAAC), and the tetrazine ligation. This thesis focuses on the development of new bioorthogonal reactions and reagents. In part 1, 5-membered cyclic Diels–Alder dienes are studied. This study ranges from the development of 4H-pyrazoles as bioorthogonal reagents to …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… that can be labelled via reactions with both tetrazine- and azide-conjugated probes. I demonstrated that BCN-K could be incorporated at a range of sites in soluble recombinant E and intact virions and that these proteins could be site-specifically labelled with fluorophores and biotin. These …
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Synthesis of derivatizable semiconductor nanocrystals through rational ligand design, and applications thereof
… ligand (PIL), and that is reactive with tetrazine and, upon thermal activation, with thiols to form stable bioconjugates.
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Syntheses of biocompatible luminescent nanocrystals for visible and short-wave infrared imaging applications
… phase transferred into water via norbornene/tetrazine click chemistry, meanwhile allowing flexible functionalization of the QDs by incorporating a functional group on the hydrophilic chain. This ligand system can be applied to a variety of carboxylic-ligand-stabilized QDs, with emission …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
… allyl-modified nucleosides and a dipyridyl tetrazine was developed and characterised. This reaction uniquely generates a sterically bulky double-addition product for all nucleosides explored. To facilitate the creation of the necessary tools for this work, a highly efficient, single-step …
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Exploring the Structural, Magnetic, Optical, and Catalytic Properties of High-Spin Materials
… and Tb-MOFs were synthesized from 4,4′-(1,2,4,5-tetrazine-3,6-diyl)dibenzoic acid (H₂TZDC). ac magnetic studies revealed the MOFs exhibit complex temperature-dependent slow magnetic relaxation, attributed to combined quantum tunneling (QTM), Orbach, and Raman mechanisms. Notably, the Gd-based …