Global ETD Search
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Showing 1 to 16 of 16 for “"Tetrahymena thermophila"”.
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Recombination in Tetrahymena Thermophila
Made available in DSpace on 2014-12-14T16:11:12Z (GMT). No. of bitstreams: 1 7714982.pdf: 3650716 bytes, checksum: 6e921602d05885acad0fe8b3c9114392 (MD5) Previous issue date: 1977
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Developmentally regulated SUMOylation in the ciliate Tetrahymena thermophila
… in the developing macronucleus (anlagen) of<em>Tetrahymena thermophila.</em> Immunoblotting of cell lysates from vegetative and mating cells using anti-SUMO antibodies revealed distinct developmental differences and an increased signal correlated with formation of the anlagen. Immunofluorescence …
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Biochemical Studies of Ribonuclease P of Tetrahymena Thermophila
Photochemical crosslinking experiments with a photoaffinity-labeled substrate have been performed with the RNase P RNA subunit and holoenzymes from various sources. Experimental studies clearly reveal that the prokaryotic RNA subunit is involved in substrate binding, but that the eukaryotic RNA …
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Long-Term Evolution of the Complex Eukaryote Tetrahymena thermophila
… sex and populations that hadn’t, of the ciliate Tetrahymena thermophila at two temperatures and followed changes in growth rate over 1000s of generations. As expected, growth rate increased with a decelerating rate for all populations; however, there were differences in the patterns of evolution …
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Analysis of DIE5 and LIA5 reveals the importance of DNA repair in programmed DNA rearrangement of Tetrahymena thermophila
… differentiation, the single cell eukaryote Tetrahymena thermophila undergoes genome-wide programmed DNA rearrangement to eliminate transposon-like elements from its future soma. This process involves small RNA-directed heterochromatin formation followed by extensive nuclear reorganization to …
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Effects of altered expression of the sumo conjugating enzyme, UBC9 on mitosis, meiosis and conjugation in Tetrahymena thermophila
… repair and mitosis. The segregation of the <em>Tetrahymena thermophila</em> genome into two different nuclear compartments provides an unusual context for the analysis of SUMOylation. Each cell contains a transcriptionally silent, diploid germ line micronucleus (MIC) that divides by mitosis and …
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MyTH4 and FERM Have Overlapping and Distinct Roles in the Function of Myo1, a Class XIV Myosin in Tetrahymena thermophila
… XIV, XV and in MyoG. Myo1, a class XIV myosin in Tetrahymena thermophila, contains MyTH4 and FERM. Previous studies have shown that Myo1 localizes to phagosomes, the cytoskeleton, and the macronucleus, and that phagosome trafficking and division of the macronucleus are affected in a MYO1 knockout. …
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LIA4 and HP1-like Proteins Facilitate DNA Elimination During Tetrahymena Macronuclear Development
<p>As part of its sexual development, the ciliate Tetrahymena thermophila establishes a differentiated somatic genome called the macronucleus. A key aspect of this differentiation is the genome-wide removal of transposon-like and other repetitive elements. This process, referred to DNA …
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Computational prediction of RNA-based gene regulatory mechanisms in human and Tetrahymena
… class of sRNAs isolated from the ciliate Tetrahymena thermophila, present functional comparison to known classes of sRNAs in other organisms, and note a strong and specific relationship to a novel sequence motif. In the second section, we examine the evolutionary impact of microRNAs …
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Higher-order interaction inhibits bacterial invasion of a phototroph-predator microbial community
… reinhardtii (phototroph) or the ciliate Tetrahymena thermophila (predator), but cannot invade a community where both are present. The invasion resistance of the algae-ciliate community arises from a higher-order (3-way) interaction that is unrelated to resource consumption. We show that …
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Analysis of Behavior in Populations of Swimming Microbes
… easily. We chose to work with a microbe, Tetrahymena thermophila, as a model organism for these studies; it is easy to grow in the laboratory in controlled conditions, has a relatively short generation time, and is large enough to for its motions to be easily imaged. To achieve the …
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Gpi-Anchored Proteins In Ciliated Protozoa: From Ca++ Signaling To Mitochondrial Extrusion
… of cell biology and genetics, most notably, Tetrahymena and Paramecium. One of the characteristic features of ciliates is their extensive cell surface comprised of plasma and ciliary membranes enriched in GPI-anchored proteins known as immobilization antigens or i-antigens. Antibodies against …
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Population Dynamics In A Model Closed Ecosystem
… Escherichia coli, Chlamydomonas reinhardtii and Tetrahymena thermophila and show that these species can coexist for hundreds of days under closure. Using a custom built in situ fluorescence microscopy set up, the densities of these three species can be measured automatically and noninvasively …
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Evaluating the emerging environmental risks of mycotoxins in freshwater ecosystems.
… Lymnaea stagnalis (embryos) and Protozoa Tetrahymena thermophila. The resulting inhibition values in terms of growth, immobilisation survival or reproduction demonstrated DON poses the greater toxic hazard to crustaceans, whereas ZON was most toxic to mollusc embryos and cnidarians. The …
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The Impacts of Disruptive Environmental Change on Vital Microbial Ecosystems
… on body size (M) and density (N) in <i>Tetrahymena thermophila</i>, based on a unified differential equation model. Our findings highlight the crucial role of environmental conditions in shaping the body size and density of <i>T. thermophila</i>, emphasizing the intricate effects of …