Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"TetR"”.
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Optimizing a protein-RNA aptamer gene regulatory system using an engineered peptide library
… advantage of the reversible interaction between TetR and its RNA aptamer binding partner 5-1.2 to modulate gene expression. With no tetracyclines present, TetR preferentially binds to aptamer 5-1.2 in the mRNA of a gene of interest with low nanomolar affinity and represses translation. …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… binding to the bacterial Tet Repressor protein (TetR) with low- to sub- nanomolar affinities were identified. This interaction is reversibly controlled by tetracycline in a manner analogous to the interaction of TetR with its cognate DNA operator. Aptamer minimization and mutational analyses …
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Identification and characterization of farR and farE as a regulator and effector of fatty acid resistance in Staphylococcus aureus
… substitution in an uncharacterized member of the TetR family of transcriptional regulators, which is divergently transcribed from a gene encoding a member of the resistance-nodulation-division superfamily of multi-drug efflux pumps. I named these genes farE and farR, for regulator and effector of …
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Engineering control of eukaryotic translation with application to the malaria parasite Plasmodium falciparum
… interaction between an RNA aptamers and the TetR-repressor protein. Although such protein-RNA interactions are abundant in nature and are known to effectively mediate control of gene expression, our system is unique in its direct modulation by an exogenous chemical. By genetically encoding …
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Characterization, toxicity, and biological activities of organometallic compounds and peptide nucleic acids for potential use as antimicrobials
… vitro effects of CPP-PNAs targeting the tetA and tetR antibiotic resistance genes (CPP-anti-tetA PNA and CPP-anti-tetR PNA, respectively) in tetracycline-resistant Salmonella enterica ssp. enterica serovar Typhimurium DT104 (DT104). Through the use of modified minimum inhibitory concentration …
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Determining The Genotype-Phenotype Connection In Synthetic Inducible Gene Expression Systems
… reaction models from known behaviors of the <em>tetR</em> and <em>rtTA</em> genes, gene products, and their gene architectures. I then simplified these models to account for essential behaviors of gene expression systems. Finally, I used these models to predict behaviors of modified gene …
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Biomolecular and computational frameworks for genetic circuit design
… circuits design. In this thesis, we explore TetR-family repressors and the CRISPRi system as candidates. First, we applied 'part mining' to build a library of TetR-family repressors gleaned from prokaryotic genomes. A subset were used to build synthetic 'NOT gates' for use in genetic …
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Spatiotemporal Characterization of Stochastic Bacterial Growth in Biofilm Environment
… receiver (Chapters 3 and 5) or a LacI- and TetR-driven genetic toggle switch (Chapter 4). Under the culture conditions, the quorum sensing reporter signal was detected even without addition of the signaling molecule (Chapter 3). The genetic toggle switch was stable when the system began in …
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Advancements in genome engineering and applications in mammalian systems
… on comparing Tet Operator (TetO)/Tet Repressor (TetR) based imaging method with TALE and CRISPR-Cas9 mediated loci labeling methods. Signal to nuclear noise ratios (SNRs) were analyzed first. The high SNRs indicated all methods should be suitable for live cell imaging applications. Labeled loci …
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Gene der Chinaldin-Degradation in Arthrobacter ilicis Rü61a: Charakterisierung und funktionelle Expression der Chinaldin-4-Oxidase Strukturgene
… eine Mandelat-Racemase, einen TetR-ähnlichen Transkriptionsrepressor und zwei Transmembranproteine.
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… DNA-binding domains, such as Tet Repressor (TetR) or Zinc Finger (ZF) proteins. Optimized EGTs will enable the production of persistent, tunable CGTs without disrupting the endogenous genome.
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REPSA Directed Assessment of Native Cleavage Resistance of DNA to Type IIS Restriction Endonucleases and Modification of REPSA for High Temperature Application
… as a model thermophilic organism. We used a TetR Family (TFR) transcription factor SbtR as the model protein for optimization of HT-REPSA protocols, as data had previously been obtained regarding SbtR physical characteristics and DNA-binding properties. REPSA was conducted until a cleavage …
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Creating a New Malaria Vaccine Design that uses a Blood Stage P. falciparum Chassis for Non-Blood Stage Antigen Presentation
… the conditional translation repressor system TetR-DOZI were created, and transgenic parasites that express the scaffold protein eTRAMP4 fused to either CSP or P25 were generated. We assessed the transgenic parasites for growth defects, proper fusion length, and localization to the …
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Cepas clínicas de SARM de líneas genéticas asociadas a animales: epidemiología, genómica, proteómica y nuevos antimicrobianos
… fenotípico de SARM CC398 es la resistencia a tetraciclina (TetR). Otra línea genética emergente de SARM-AG es la del CC130, generalmente portador del gen mecC de resistencia a meticilina, y característica por presentar un fenotipo de sensibilidad a los antibióticos no β-lactámicos (SNBL). El …
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Líneas genéticas, virulencia y resistencia a antibióticos en Staphylococcus aureus de diferentes orígenes: Análisis de marcadores de adaptación al huésped y comportamiento en Caenorhabditis elegans
… objetivo fue determinar si la resistencia a la tetraciclina (TETR) era un buen marcador de la línea genética CC398 en aislados SARM y asimismo si el contacto con animales de granja era un factor de riesgo de colonización y/o infección por SARM-CC398. Para ello, se caracterizaron todos los …
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Engineering Signal Transduction Pathways in Bacteria
… verwendet: a) Kompetition von OmpR-P mit dem TetR Repressor, expremiert von einem synthetischen Promotor, zur Aktivierung des pompC Promotors, b) Expression von Antisense RNA für das Reportergen (GFP) and c) einen bistabilen Schalter durch TetR und einem temperatur-abhängigen Protein CI, …
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Molecular and transcriptomic studies of quorum sensing in Chromobacterium subtsugae
… genes are repressed by an adjacently encoded TetR-family transcriptional regulator, CdeR. Our research also demonstrates that CviR and CdeR independently regulates the expression of cdeAB-oprM genes by directly interacting with the cdeA promoter. CviR requires a lux-box and CdeR requires a …
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Harnessing mutagenic homologous recombination for in vivo targeted mutagenesis by TaGTEAM
… yeast 3-methyladenine DNA glycosylase MAG1 to a tetR DNA binding domain, we are able to elevate mutation rates > 800-fold in a specific ~20 kb region of the genome or on a plasmid that contains an array of tetO sites. A wide spectrum of transitions, transversions, and single base deletions are …
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Evolution-Inspired Design of Bacterial Biosensors
… that confer DNA-binding specificity to the TetR family of TFs. Finally, in the third portion of this work, we develop new methods for analyzing signal transduction in bacterial TCSs. We characterize interactions between membrane-bound sensor histidine kinases (SKs) and their cognate response …
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Analysis of alkali-inducible genes of Bacillus subtilis
… for a transcriptional regulator of the IcIR and TetR/AcrR family while the pspA encode a transcriptional anti-activator in E. coli . In Northern blot analyses, it could be shown that all three genes are induced after alkali shock. The transcription start points of the kipR and yvdT genes were …
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