Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 118 for “"Tertiary structure"”.
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Physicochemical Studies on the Tertiary Structure of Bovine Serum Albumin
Made available in DSpace on 2014-12-10T23:00:53Z (GMT). No. of bitstreams: 1 7105140.pdf: 3716271 bytes, checksum: dd2a25143de195172b282175392fe351 (MD5) Previous issue date: 1970
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Automated and accurate description of protein structure -- from secondary to tertiary structure
<p>The automated protein structure analysis (APSA) has been developed that describes protein structure via its backbone in a novel way. APSA generates a smooth line for the backbone which is completely described using curvature κ and torsion τ as a function of arc lengths. Diagrams of κ(s) and τ(s) …
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Methods to program and to probe RNA tertiary structure with nucleic acid origami
Biological structure determination has revolutionized mechanistic understandings, nanotechnology, and drug design. Despite advances in structural determination technologies, from nuclear magnetic resonance to cryo-electron microscopy (cryo-EM), one class of biomolecules has resisted 3D structure …
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The Use of a Bifunctional Reagent in The Study of The Tertiary Structure of Bovine Pancreatic Ribonuclease
Made available in DSpace on 2014-12-05T19:38:20Z (GMT). No. of bitstreams: 1 6402891.pdf: 3666384 bytes, checksum: 2f2e9330ae15ec44e2e2f243565f627b (MD5) Previous issue date: 1963
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The Role of Secondary and Tertiary Structure in the Cap-Independent Translation of FGF-9 and HIF-1-alpha
… sequence similarity or conserved secondary structure has been proposed as DAP5 or eIF4G binding sites. RNA structure, however, has previously been found to bind initiation factors in viral IRES. Using selective 2’ hydroxyl acylation by primer extension (SHAPE), I found that the FGF-9 5’ UTR …
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Data mining of tetraloop-tetraloop receptors in RNA XML files
RNA (Ribonucleic acid) Motifs are tertiary structures that play an important role in the folding mechanism of the RNA molecule. The overall function of a RNA Motif depends on its specific bp (base pairs) sequence that constitutes the secondary structure. Data mining is a novel method in both …
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The impact of variation in freezing and thawing process parameters on the critical quality attributes of a monoclonal antibody
… on protein aggregation and perturbations in tertiary structure. Results showed that the processing parameters studied caused significant variation in freeze and thaw process times, with the factors causing slower rates of freeze and thaw also shown to cause changes in the tertiary structure …
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Computational Investigations of Cellular Functions: Three Cases on Membrane Morphogenesis, Organization and Assembly of a Multi-Protein Complex, and the Molecular Origin of Muscle Elasticity
… soft, entropic spring-type elasticity; secondary structures of titins immunoglobulin-like (Ig-) and fibronectin type III-like (FN-III) domains provide a stiff elasticity. We demonstrated that titin exhibits a third type of elasticity due to tertiary structure and involving domain-domain …
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A Computational-Experimental Study of Small Globular Proteins
… probes to study the formation of secondary and tertiary structure of proteins is stressed to obtain a description of their folding. Molecular dynamics simulations are carried out under non-physiological conditions to overcome experimental obstacles. Under physiological conditions, the …
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A Computational - Experimental Study of Small Globular Proteins
… probes to study the formation of secondary and tertiary structure of proteins is stressed to obtain a description of their folding. Molecular dynamics simulations are carried out under non-physiological conditions to overcome experimental obstacles. Under physiological conditions, the …
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Characterization of a single-chain anti-T cell receptor antibody and a single-chain T cell receptor
… containing a variable and a constant domain. The tertiary structure and binding properties (e.g. affinity and kinetics) of the TCR have not been solved. Additionally, the T cell has been implicated in many autoimmune diseases and some reports show that antibodies to the TCR may possibly control …
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Mass spectrometric indentification of formaldehyde-induced modifications of peptides and proteins under in vivo protein cross-linking conditions
… in model proteins that contain a more complex tertiary structure. It was shown that the extent of formaldehyde reactivity was dependent on the state of protein unfolding, i.e., solvent accessibility of reactive residues, and that an unfolded protein showed a significantly higher number of …
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Protein MAS NMR methodology and structural analysis of protein assemblies
… particular emphasis on the analysis of protein structure, are described in this thesis. MAS NMR studies of biomolecules ranging from model peptides and proteins in crystalline form to amyloid fibrils and whole bacterial organelles are reported. The methods presented include novel pulse sequences …
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ChaperoNet: Distillation of Language Model Semantics to Folded Three-Dimensional Protein Structures
Determining the structure of proteins has been a long-standing goal in biology. Lan- guage models have been recently deployed to capture the evolutionary semantics of protein sequences, and as an emergent property, were found to be structural learn- ers. Enriched with multiple sequence alignments …
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Computational Studies on the Mechanical Properties of Muscle and Blood Clot Elasticity
… to measure the free energy landscape of tertiary structure transformations. Several domains of titin are investigated in detail: the Z1Z2 domains bound to the protein telethonin at one end of the long protein chain in a relatively immobile region of the muscle fiber, and the I91 domain …
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DEVELOPMENT OF VIRTUAL SCREENING METHODOLOGIES FOR IDENTIFYING RNA-BASED THERAPIES
… RISoTTo (RIbonucleic acid Sequence design from TerTiary structure) framework to handle conformationally flexible RNA molecules, enabling context-aware RNA sequence design. The integrated computational framework presented in this work aims to advance the understanding and practical prediction of …
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Structural characterization of the C-terminal domain of human nucleophosmin, NPM1
… EACH ENCOMPASSING 10-12 AMINO ACIDS. IT FORMS A TERTIARY STRUCTURE RESEMBLING A TRIANGLE WITH N AND C-TERMINI OF THE DOMAIN COMING TOGETHER. THIS STRUCTURE WILL SERVE AS A PEDESTAL TOWARDS MAPPING THE REGIONS IN NPM1 INVOLVED IN BINDING TO P53. THE INFORMATION OBTAINED WILL PROVIDE AN INSIGHT …
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Molecular Mechanisms of Cell Adhesion
… steered molecular dynamics. Starting from the structure of the complex from the Ig1-Ig2-Ig3 fragment, we first equilibrate the complex in solvent and show that its actual end-to-end length is markedly larger than in the crystal structure. We then show that the Ig12/Ig12 complex can behave as a …
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Regulation of E. coli SOS mutagenesis by dimeric intrinsically disordered umuD gene products
… proteins, which lack a defined secondary or tertiary structure when purified and many of which have important roles in regulation. Counterintuitively, UmuD2 and UmuD'2 form stable homodimers in vitro at concentrations where little or no a helix or 13 sheet is detectable. High protein …
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PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
… with a molecular weight around 61 kD. The tertiary structure of VGLUT1 is still unknown. In our study, recombinant VGLUT1 was expressed in Pichia pastoris and purified using either a nickel chelating column or cobalt-coated Dynabeads. The HiTrapTM nickel chelating column proved to be more …
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