Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Tegument"”.
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The Roles Of Human Cytomegalovirus Tegument Proteins Pul48 And Pul103 During Lytic Infection
<p>THE ROLES OF HUMAN CYTOMEGALOVIRUS TEGUMENT PROTEINS pUL48 AND pUL103 DURING LYTIC INFECTION</p> <p>by</p> <p>DANIEL A. ORTIZ</p> <p>December 2015</p> <p>Advisor: Dr. Philip E. Pellett</p> <p>Major: Immunology and Microbiology</p> <p>Degree: Doctor of Philosophy </p> <p> Human cytomegalovirus …
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Investigating the structure and function of HSV-1 tegument proteins: UL7 and UL51
… is surrounded by a protein-rich layer, termed tegument, and a membranous envelope containing viral glycoproteins. HSV-1 genome replication and encapsidation occurs in the nucleus, after which, DNA-loaded capsids enter the cytoplasm where they undergo tegumentation and assembly. This thesis …
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MECHANISM AND STAGES OF PACKAGING OF VP8, THE MAJOR TEGUMENT PROTEIN OF BOVINE HERPESVIRUS-1
VP8 (pUL47), the major tegument protein of bovine herpesvirus -1 (BoHV-1), is crucial for viral replication and induction of host immune responses. VP8 (pUL47) translocation from the nucleus to the cytoplasm and subsequently to the Golgi results from its phosphorylation within the nucleus by pUS3. …
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Structural and biochemical characterisation of pUL7 and pUL51, conserved tegument proteins required for efficient herpesvirus assembly
Herpesviruses are ubiquitous viral pathogens that infect all known vertebrates. It is estimated that over 90% of adults worldwide are infected with at least one of the nine human herpesviruses, which cause a wide range of morbidity and mortality. These viruses share a conserved assembly pathway, …
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A Role for the HSV Tegument Protein pUL16 in Modulating Cellular Energy Metabolism during HSV-2 Infection
The conserved, multifunctional tegument protein, pUL16, is implicated in several processes during Herpes simplex virus (HSV) infection, including virion morphogenesis and cell-to-cell spread of infection. Recent literature has also demonstrated that HSV-1 pUL16 localizes to distinct regions of …
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Functional Studies of Stem Cells in the Parasitic Flatworm Schistosoma mansoni
… characterized by a syncytial "skin" known as the tegument that is critical for their survival within their hosts, they possess somatic stem cells referred to as neoblasts, and they are incredibly successful parasites. Until now, there has been little appreciation for the interrelationships between …
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PROTEASOME-DEPENDENT ENTRY OF HERPES SIMPLEX VIRUS
… properties of ICP0 that is present in the virion tegument layer have not been well characterized. For these reasons, I sought to characterize tegument ICP0 and determine the role of tegument ICP0 during proteasome-dependent entry of herpes simplex virus. Protein compositions of wild-type and ICP0 …
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Development of a conditionally-attenuated cytomegalovirus (CMV) vaccine vector platform, and initial investigations into use of CMV as a vaccine against pandemic influenza A virus
… on a hypothesis that targeting of essential CMV tegument proteins by using regulatable protein-destabilization is suitable as a conditional vaccine attenuation strategy. CMVs are generally benign, however in individuals whose immune systems are immature or weakened, CMV can be a significant …
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The cellular and molecular origins of extracellular vesicles secreted by <i>Fasciola hepatica</i>
… sites of FhEV production (the parenchyma, tegument, gastrodermal cells and protonephridial system) and indicated that further subtypes of FhEVs exist within the previously characterised 15K and 120K EV populations. Additionally, ultrastructural analysis of these tissues identified several …
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Understanding the function and structure of herpes simplex virus type 1 pUL55, a novel immune evasion protein
… infection. This work focusses on pUL55, a HSV-1 tegument protein, which we hypothesised to be an innate immune modulator with similar functions to ICP0. ICP0 exhibits E3 ubiquitin ligase activity and is known to stimulate proteasomal degradation of several host proteins in HSV-1 infected cells. …
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Viral Factors Influencing Early Infection Events During Ex-Vivo KSHV Infection
… <p>Establishing the Contribution of KSHV-ORF11 Tegument Protein in de novo</p> <p>Infection of B Lymphocytes.</p> </li> </ol> <p>We have demonstrated that gamma-herpesvirus conserved tegument factors play critical but uncharacterized roles in the establishment of infection in B lymphocytes, and …
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Multi-modal imaging of herpesvirus infection
… nuclear membranes, and acquire a proteinaceous ‘tegument’ layer in the cytoplasm before becoming enveloped at trans-Golgi network vesicles and/or recycling endosomes. Although the virus assembly pathway has been studied in detail using fluorescence and electron microscopy, several aspects of the …
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The development of the rumen fluke, Calicophoron daubneyi, within the mammalian host
… the ultrastructural level, with a focus on the tegument, digestive system, excretory system and reproductive structures. The fine structure of the tegument suggests its major role is protection since it lacks mitochondria and thus may not participate in active uptake of molecules from the host …
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Adequação da metodologia do teste de tetrazólio para sementes de Aspidosperma pyrifolium mart
… paper sheet 2,5 times its weight at 25 ºC. Seed tegument was left out and embryo subjected to tetrazolium solution, to coloring, on concentrations at 0,025; 0,050; 0,075 e 0,1% during 30, 60, 90 and 120 minutes, on darkness in B.O.D. at 40ºC. After longitudinal separation among cotiledon and …
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Human Cytomegalovirus Us17 Locus Fine-Tunes Innate And Intrinsic Immune Responses
… seen in the absence of the immunomodulatory tegument protein pp65 (pUL83). Although ΔUS17 produced equal numbers of infectious particles in fibroblasts compared to parental virus, at equal multiplicities of infection, it produced >3-fold more genome-containing non-infectious viral particles, …
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Identification of Epstein-Barr virus proteins that inhibit the DNA damage response
… etoposide-induced DNA damage. An uncharacterized tegument protein, BKRF4, was identified as an inhibitor of the DNA damage response. By studying the effect of BKRF4 on the steps of the DDR, it was shown that BKRF4 had no effect on the induction of γ-H2AX, but blocked the subsequent step at which …
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Patterns of Gene Expression in Schistosoma mansoni larvae associated with Infection of the Mammalian Host
… the mammalian host. The transcription of many tegument and gut-associated genes was also increased in the schistosomulum; cathepsins were particularly notable, even in the cercaria, implying that the larval gut becomes active long before blood feeding begins. The first application of whole …
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Glycoprotein M and ESCRT in herpes simplex virus type 1 assembly
… cell-derived lipid envelope and a proteinaceous tegument layer. There are over fifty viral proteins and many host proteins identified in HSV-1 virions. The final formation of mature virus particles requires the membrane wrapping of tegumented capsids in the cytoplasm, a process termed secondary …
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Investigating dynein-based transport of intracellular pathogens
… Previous studies have indicated that the viral tegument protein UL36 is responsible for the recruitment of both dynein and kinesin motors. I demonstrate that ectopically expressed UL36 exhibits different transport behaviours in different cell types and discuss potential strategies to investigate …
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