Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Targeted metabolomics"”.
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Synthesis and application of tagging agents in mass spectrometry-based targeted metabolomics studies
Metabolomics is a technique which provides comprehensive metabolite profiling as well as metabolic pathway elucidation and potentially has a wide range of applications for medical research including disease diagnosis and monitoring. In this thesis, some synthetically prepared and existing tagging …
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Gluconeogenic FBP1 plays a key metabolic role in activated T cells
… plays a novel non-gluconeogenic role in T cells. Targeted metabolomics using [13C]-6-glucose revealed a labeling pattern of F6P in stimulated CD3+ T cells that could only have resulted from FBP1 enzymatic activity. Following stimulation, T cells expressed a 27kD form of FBP1, in addition to the …
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Obesity and Health in the CHRIS study
… studies (GWAS). We then paid attention to the targeted metabolomics data of the CHRIS study, to identify different serum metabolites associated with metabolically healthy/unhealthy obesity, using logistic regression and random forest methods to explore metabolic signatures to distinguish …
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LINEAGE TRACING REVEALS PAEP AS A CHEMOTHERAPY-INDUCED METASTATIC DRIVER AND EXPOSES METABOLIC VULNERABILITIES OF CIRCULATING TUMOR CELLS IN BREAST CANCER
… enabling matched multi‐compartment analyses. Targeted metabolomics and Seahorse assays profiled metabolic states. Functional tests included PAEP knockdown and transient pharmacologic modulation of mitochondrial activity in freshly isolated CTCs. AC reduced clonal diversity at the primary site …
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Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects
… unusual pathway was regulated I carried out a targeted metabolomics analysis in our isogenic tumor cell models. This led to the striking discovery that cells engaged in the reductive carboxylation pathway also operate an additional metabolic pathway that, at first glance, would appear to be …
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Linking microbial metabolism and organic matter cycling through metabolite distributions in the ocean
… remain largely unknown. In this dissertation metabolomics techniques were developed and used to profile and quantify a suite of metabolites in the field and in laboratory experiments. Experiments were run to study the way a specific metabolite can influence microbial metabolite output and …
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Sample preparation methodologies for MALDI-MS imaging and related topics.
… relatively new addition to the 'omics sciences, metabolomics. A non-targeted metabolomics approach has been used to study both plant and animal tissue in an attempt to gain a greater understanding of the complex biological processes that occur within both types of tissue. Wheat grain was used as …
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Shotgun metagenomics exploration of fermented food microbiomes
… over recent years. These chapters also combine targeted metabolomics, and with the combination of shotgun metagenomics, examines the interplay between microorganisms, kombucha chemistry, and oxygen transfer rate. Chapter 5 explores the microbiomes of 91 fermented foods from Africa. African …
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Linking microbial metabolism and organic matter cycling through metabolite distributions in the ocean
… remain largely unknown. In this dissertation metabolomics techniques were developed and used to profile and quantify a suite of metabolites in the field and in laboratory experiments. Experiments were run to study the way a specific metabolite can influence microbial metabolite output and …
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Improving Mass Spectrometry-Based Metabolite Identification and Quantification and Application to Cardiovascular Disease
… have been taken in these applications: targeted and non-targeted profiling. The targeted approach identifies and quantifies select known or potential biomarkers in CVD, often via isotope-labeled internal standards. The non-targeted approach attempts to profile the full spectrum of the …
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Examining coral reef ecosystem dynamics using microorganisms and metabolites
… Finally, in Chapter 5, I combined analyses of untargeted and targeted metabolomics, microbial taxa, and functional genes from metagenomics across 300 km of reefs in Florida, in addition to microorganisms in healthy and diseased corals. With this unprecedented combination of ‘omics datasets, I …
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Metabolic control of the ethylmalonyl-CoA pathway in Methylobacterium extorquens AM1
… generating a timecourse of response measured by targeted metabolomics, RNA-seq transcriptomics, and enzyme activities. From these experiments, ethylmalonyl-CoA mutase was identified as a metabolic control point. To confirm the role of this enzyme as a metabolic control point, over-expression of …
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Examining coral reef ecosystem dynamics using microorganisms and metabolites
… Finally, in Chapter 5, I combined analyses of untargeted and targeted metabolomics, microbial taxa, and functional genes from metagenomics across 300 km of reefs in Florida, in addition to microorganisms in healthy and diseased corals. With this unprecedented combination of ‘omics datasets, I …
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NONHUMAN PRIMATES AS A TRANSLATIONAL MODEL OF ENDOMETRIOSIS AND ITS EFFECTS ON WOMEN'S HEALTH
… mitochondrial respirometric analysis and targeted metabolomics. The effects of endometriosis on systemic iron metabolism were identified using NHP and human medical records, NHP tissue histomorphometry, and serum ELISAs. In endometriosis tissue, macrophage density correlated with nerve …
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Identification of Early Diagnostic and Predictive Biomarkers of Cisplatin-Induced Acute Kidney Injury Using Metabolomics
… high risk patients prior to cisplatin therapy. Untargeted metabolomics was utilized to characterize the metabolic alterations induced by cisplatin in a mouse model of cisplatin-induced nephrotoxicity, an adult cohort of head and neck cancer patients receiving cisplatin, and a cohort of pediatric …
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Metabolomics of Mammalian and Cellular Models of Aging
… Here, we report changes in the both global and targeted metabolomics (the study of small molecules) in the brains of the Ts65Dn mouse. We also report that long-term treatment with microencapsulated dietary rapamycin changes the metabolomic profiles in a manner consistent with increases in …
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Later Life Consequences of Developmental Mitochondrial DNA Damage in C. elegans
… changes are measured via real time PCR and targeted metabolomics via LC-MS are used to investigate changes in organic acid, amino acid and acyl-carnitine levels. Lastly, nematode developmental delay is assessed as growth, and measured via imaging and COPAS biosort.</p><p>I have found that …
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Applications of metabolomics to study the pathophysiology of adverse pregnancy outcomes
Background: Clinical metabolomics is a growing field of research aiming to use metabolomic techniques to gain further knowledge into diseases, the use of biomarkers to predict their onset, or the effect of a potential therapeutic agent on the metabolome. Adverse pregnancy outcomes, such as small …