Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 45 for “"Target mRNA"”.
-
Molecular titration by MicroRNAs and target mimic inhibitors
… manner. Each miRNA is predicted to target hundreds of genes, and a majority of protein-coding genes are computationally predicted to be miRNA targets. To test miRNA functions experimentally, we introduced the miRNA "sponge" method, which uses miRNA target mimics to sequester mature …
-
Post-Transcriptional Regulation of Mammalian Gene Expression In Non-Coding Region of Target Rna
… in the human chromosome 3p21.3 region. TUSC2 mRNA transcripts could be detected on Northern blots in both normal lung and some lung cancer cell lines, but no endogenous TUSC2 protein could be detected in a majority of lung cancer cell lines. Mechanisms regulating TUSC2 protein expression and …
-
Translational regulation by short RNAs in mammalian cells
… major generalized roles in the regulation of mRNAs which are typically 100 times larger in size. These short RNAs, siRNAs and microRNAs, exert their influence on gene expression in mammals by RNA interference (RNAi) and translational repression. RNAi initiated by exogenous siRNAs results in …
-
MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS
… level by binding to the 3'UTR region of a target mRNA and either preventing its translation or causing its degradation. MicroRNA have been shown to be involved in cell differentiation in other systems implying that they may also have a role in the changes that occur within granulosa cells …
-
Dysregulated DJ1-Mediated Translation in Alzheimer's Disease
… during healthy and disease states. The mammalian target of rapamycin (mTOR) is a ubiquitously expressed kinase that governs protein synthesis, and is required for learning and memory. Here, we explore a downstream target of mTOR, the novel RNA-binding protein (RBP) Parkinson Protein 7 (DJ1) (Niere …
-
Designing nanoparticles for highly efficient endothelial siRNA delivery
… avoid phagocytosis in the bloodstream, reach the target tissue, and get into, and out of, an endosome, all without setting off an unwanted immune response. Despite these challenges, nanoparticles have delivered siRNA to hepatocytes after intravenous injections as low as 0.001 mg/kg. By contrast, …
-
MicroRNAs in cellular transformation and tumorigenesis
… this regulation is achieved via interaction with target messenger RNAs (mRNAs), largely through their imperfect base pairing with the 3' untranslated regions (3' UTRs) of these target transcripts. Through this imperfect base pairing, miRNAs induce a repression of mRNA translation that is …
-
Expression analysis of Drosophila melanogaster microRNAs
… regulieren die Translation distinkter zellulärer mRNAs über die Ausbildung zumeist nicht vollständig gepaarter Duplexregionen. Auf Grund der Komplexität der target mRNA Erkennung ist bisher nur ein kleiner Teil der miRNAs mit einer biologischen Funktion annotiert worden. Um die Aufklärung dieser …
-
Thermodynamics and kinetics of antisense oligonucleotide hybridization to a structured mRNA target
… These early studies have shown that selection of target site, once considered a trivial problem, is critical to the success of antisense strategies. It has become clear that the efficacy of antisense oligonucleotides is a strong function of the structure of the target mRNA. Though single-stranded, …
-
Identification and characterization of gene and microRNA networks associated with cancer survival and drug abuse
… and therapeutical remedies, as well as targets for future basic and applied research. The identification of reliable transcriptome biomarkers requires the simultaneous consideration of regulatory and target elements including microRNAs (miRNAs), transcription factors (TFs), and target …
-
Screening, synthesis, and applications of "lipidoids", a novel class of molecules developed for the delivery of RNAi therapeutics
… and systemic administration effectively silenced target genes in three different tissue types. In addition to reducing protein expression by directing cleavage of target mRNA through delivery of siRNA, lipidoids were shown to mediate upregulation of proteins by derpressing miRNA targets through …
-
Correlation Between Computed Equilibrium Secondary Structure Free Energy and siRNA Efficiency
… secondary structure of the participating mRNA, siRNA, and their complexes. A previously published data set of 609 experimental points was used for the analysis. While virtually no correlation with the computed structural signatures are observed for individual data points, several clear …
-
Stochastic modelling of gene regulation with microRNAs
… researchers are focusing on identifying the targets of microRNAs and the roles of microRNAs in post-transcriptional gene regulation; the work presented in this thesis is a contribution to this field. Here, a range of kinetic models of gene regulation is studied computationally with a view to …
-
Exploring the specificity and mechanisms of siRNA-mediated gene silencing in mammalian cells
… knockdown gene expression. siRNAs bind to their target sequence and guide transcript cleavage and subsequent degradation. This type of silencing is associated with equivalent levels of RNA and protein knockdown. siRNA-mediated knockdown was originally thought to be highly specific. However, the …
-
The Role of the ORB2 RNA-binding Protein in Post-transcriptional Regulation
… determine the translational fate of their RNA targets. A wide variety of post-transcriptional regulatory mechanisms are employed during the early Drosophila embryo and it has been established as a system well suited for the study of these mechanisms. In Drosophila, ORB2 is an RBP expressed in …
-
Investigating the role of the post-transcriptional regulator protein ZFP36L1 in B-cell functions
… to bind to the ARE in the 3’ UTR regions of the target mRNA (via the zinc finger domains) and subsequently result in the destabilization and degradation of the mRNA. Established targets of ZFP36L1 include TNF-a, GM-CSF, VEGF, IL-3, C-IAP2, NOTCH 1, STAR, STATSb and DIM. The knowledge about the …
-
Characterization of Helicobacter pylori sRNAs HPnc2525, HPnc2600, and HPnc2645
… in length and act independently on expressed targets. sRNAs serve to regulate and fine-tune gene expression by interacting with target mRNA molecules to inhibit or accelerate gene translation or function. Understanding how cagPAI genes are regulated is key to understanding how they promote …
-
Investigating microRNAs during cardiac development in the chick
… silencing complex, which binds to a target mRNA transcript and inhibits its translation. Dysregulation of miRNAs is associated with failing human hearts6. More than 2,500 mature human miRNAs are currently annotated, and with each miRNA predicted to potentially target several hundred …
-
An E. coli small RNA inhibits translation initiation from a distance
… repression is commonly carried out via sRNA-mRNA base pairing interactions near the Shine-Dalgarno (SD) region. In this so-called “canonical” mechanism, the sRNA is the direct regulator; it competes with the initiating ribosomes while the chaperone protein Hfq plays a supporting role. …
-
Mammalian gene regulation through the 3' UTR
… region (UTR) at the 30 end of a mammalian mRNA is typically rich with regulatory motifs that influence the stability, localization, translation and other properties of the message. We explored two classes of motifs, microRNA (miRNA) complementary sites and cleavage and polyadenylation …
Page 1 of 3