Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 88 for “"Taqman"”.
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Development of Immunomagnetic Capture (IMC) Based Techniques For the Detection of Salmonella in Poultry Carcass Rinse Fluid
… and molecular-based detection methods, PCR and Taqman® PCR employing IMC without enrichment, could detect at least 102 cfu/ml of S. Typhimurium in broiler carcass rinse fluid (CRF) samples. IMC-ELISA, IMC-PCR, and IMC-Taqman® PCR were initially tested using 0 to 106 cfu/ml of pure culture S. …
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Etablierung von Real-Time-PCR-Verfahren für die Diagnostik des Cytomegalovirus bei organtransplantierten Patienten
… <br>Im Rahmen dieser Arbeit wurden quantitative TaqMan-PCR-Verfahren etabliert. Die diagnostische Wertigkeit wurde an 80 PBL-Proben von Organtransplantierten (Niere, Niere/Pankreas, Leber) überprüft und mit Ergebnissen des pp65-Antigentests sowie dem klinischen Verlauf der HCMV-Infektion …
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Regulatory Function of MicroRNA-149 on Thromboxane A2 Receptor Gene Expression in Asthmatics
… in miR-149 (rs2292832) were genotyped using a TaqMan® allelic discrimination assay. Plasma miR-149 expression was evaluated using quantitative RT-PCR with a TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® MicroRNA Assays. The expression of the thromboxane A2 receptor (TBXA2R) gene in …
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An evaluation of genetic markers for forensic identification of human body fluids
… (LOD) were investigated using SYBR Green and Taqman probes. The results of the SYBR Green efficiency test experiment displayed five markers, UCE, TEF, ACTB, B2M, and RPS29 with 90-110% efficiency with a slope -3.33 ± 10%. Subsequently, Taqman probes were designed for the five markers and then …
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The identification of candidate genes using cDNA microarray and the analysis of two SNPs of the reelin gene in a South African austistic population
… and intronic rs736707) in the RELN gene using Taqman® SNP Genotyping assays to detect association with autism in three distinct South African (SA) ethnic groups (Black, Caucasian and Mixed), and (ii) to detect candidate genes that are over and under-expressed in the samples taken from a SA …
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DENVax live attenuated chimeric dengue vaccine
… fluorescence-based methodologies, SYBR Green and TaqMan. The SYBR Green method was used to test the virus samples and reproducible results were obtained. The TaqMan assay allows for multiplexing, which allows simultaneous detection of two PCR products; Vero cell DNA present in virus samples and an …
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Linear clustering with application to single nucleotide polymorphism genotyping
… shows promise for genotyping data generated from TaqMan technology (Applied Biosystems). A key feature of the proposed algorithm is that it applies to unnormalized fluorescent signals when the TaqMan SNP assay is used. The algorithm could also be potentially adapted to other fluorescence-based SNP …
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Instrumentation Acquisition: Do the Numbers Justify the Purchase?
… the Roche® 8800 versus two Roche® Ampliprep-Taqman Systems. A comparison is made in terms of instrumentation price, cost of reagents, and labor cost savings, while looking at affected population data to weigh whether a purchase of the more expensive of the two instruments could be justified. …
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Genetic Effect of MBL2 Polymorphisms in Patients with Diisocyanate-induced Occupational Asthma
… -225 G>C) in the MBL2 promoter were genotyped by TaqMan allelic discrimination assays using TaqMan probes. The serum MBL levels were detected using enzyme-linked immunosorbent assay (ELISA). The functional variabilities of the promoter polymorphisms were analyzed by luciferase reporter assay and …
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Neue Strategien zum Nachweis klinisch relevanter Polymorphismen
… D-Inkompatibilität ist mit Hilfe der TaqMan-Technologie durch den Nachweis von Spuren fetaler DNA im maternalen Plasma durchführbar. Die Untersuchung auf genetische Risikofaktoren für Koronarthrombosen mit einer neu entwickelten PCR-ELISA-Methode konnte keinen direkten Zusammenhang …
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Regulation of VEGF-activated signalling by the plasma membrane calcium ATPase 4 in endothelial cells
… PMCA4 knockdown have been further validated by TaqMan-based qPCR in HUVEC or HDMEC. RNA levels of PMCA4 in aging HUVEC were analysed by TaqMan qPCR using RNA isolated from HUVEC cultured from different passages (from 3 to 15 passages). RESULTS: siRNA-mediated PMCA4 knockdown led to increased …
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The candidate tumour suppressor, XIAP associated factor 1 (XAF1), directly inhibits XIAP activity and induces G1 phase cell cycle arrest
… the NCl was analyzed for xaf1 RNA expression by Taqman and heterozygosity status of markers proximal to xaf1. Taqman analysis indicated that the majority of cell lines expressed little or no xaf1 RNA while xiap levels were relatively high. A PCR study of markers near xaf1 showed significant loss …
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Prevalence of Batrachochytrium Dendrobatidis in an Amphibian Communities of Central Texas and Tamaulipas, Mexico
… presence of the pathogen was assessed by using a Taqman quantitative real time PCR (qPCR) assay in a highly sensitive approach to detection. Interestingly, there is a low prevalence for Bd in Central Texas in comparison with previous studies conducted in this area. All samples tested from …
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Bacterial Responses to Silver Nanoparticle Treatment: Community Structure, Resistance, and Function.
… wastewater treatment plants, by designing TaqMan probe assays and performing quantitative PCR. The silver resistant isolates were also used to test the growth implications, as well as sil gene expression changes, following treatment with ionic silver and a variety of silver nanoparticles …
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Genetic analysis of inherited retinal diseases in indigenous Southern African populations
… were validated by Sanger sequencing. Custom TaqMan assays were designed to screen seven mutations, identified by WES, in 193 unrelated indigenous African probands with IRDs. Results: A homozygous founder mutation, c.6377delC in MYO7A, was identified in 43% of the indigenous African patients …
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The role of seminal plasma in the regulation of inflammation and inflammatory pathways in the cervix: potential for cervical cancer progression and HIV transmission in South African women
… in neoplastic cervical epithelial cells. TaqMan 96-well array revealed that SP regulates the activation of eicosanoid, toll-like receptor-NFκB, kallikrien-bradykininbradykinin receptor, cytokine, and chemokine signaling pathways to mediate the expression of inflammatory mediators in …
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Determinanti genetici dell’espressione dell’emoglobina HbF
… nucleotide polymorphisms (SNP) were genotyped by TaqMan procedure and one by restriction enzyme, KLF1 mutation was studied by Sanger sequencing. Fetal hemoglobin (HbF) from HPFH subjects was determined using the High Performance Liquid Chromatography (HPLC). Genotype frequencies were compared with …
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The use of genetic materials, RNA, to determine the age of saliva stains
… quantified were assessed with using a pair of TaqMan® Gene Expression Assays (TGEAs). Correlations were found between the age of the saliva stain and the RQR of the β-actin mRNA to 18S rRNA in the first approach and between the age of the saliva stains and the relative quantities of two …
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Analysis of methylation status of CGB genes in common nontrophoblastic cancer
… CGB3-9 genes was investigated using qRT-PCR with TaqMan probes. Secretion in media of hCG/hCGβ was investigated with ELISA. The results demonstrated the presence of a CGI associated with CGB7 which matches previous reports of the transcription of the gene in normal and cancer tissue. Notably, this …
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Genetic and functional investigation of sequence variation associated with the related phenotypes: birth weight, obesity, and type 2 diabetes (T2D)
… methods (Sequenom iPLEX and Life Technologies TaqMan) were used to genotype specific polymorphisms in the genes HHEX-IDE, ADCY5 and FTO, while molecular cloning and computer modelling techniques were used to investigate the variants in LEPR. Results: Significant associations were found with …
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