Global ETD Search
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Showing 1 to 8 of 8 for “"Taq Polymerase"”.
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Selektive Amplifikation, Klonierung und Sequenzierung eines hypermutablen Bereiches des Fanconi-Anämie-A (FANCA)-Gens aus Fibroblasten-Kulturen unterschiedlicher Passagen und Genotypen
… Ursache ist die relativ hohe Fehlerrate der Taq-Polymerase. Für die Amplifizierung des Genomabschnittes aus dem FANCA-Gen sollte eine Polymerase verwendet werden, die eine höhere Genauigkeit als die Taq-Polymerase aufweist, wie z. B. die Pfu-Polymerase.
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Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller using RAPD
… of DNA, MgCl$\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can interfere with PCR amplification …
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Take-all in Wheat: PCR Identification of the Pathogen and the Interactions Amongst Potential Biological Control Agents
… was based on avenacinase-based primers and polymerase chain reaction (PCR) conditions specified by earlier research. PCR conditions were modified to effect detection. The annealing temperature was lowered from 68 to 62°C for plant and soil extracts, and the concentration of Taq polymerase …
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Identification and Development of Novel Compounds for the Treatment of Human Cancers
… Fluorescence Resonance Energy Transfer, with Taq Polymerase Stop assays for confirmation, a group of compounds were identified that stabilize the c-Myc G-quadruplex structure. Using a colon cancer model, two compounds were shown to decrease c-Myc gene and protein expression. Also, exposure to …
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Implémentation d'une méthode de détection du virus de la diarrhée virale bovine au sein de la fondation de la promotion des productions andines Proinpa (Bolivie)
… possible for the Master MIX (containing the Taq Polymerase (Taq)) and the Reverse Transcriptase (RT). The « Illustra Ready-To-GoTM RT-PCR Beads », from GE Healthcare, are beads made of Taq and RT that can be stored at room temperature. The combination of a lyophilized part of the LSI kit …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
The global conformations of DNA polymerase I from Thermus aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then …
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… the thermodynamics of substrate selection by DNA Polymerase I is important for characterizing the balance between replication and repair for this enzyme <I>in vivo</I>. Due to their sequence and structural similarities, Klenow and Klentaq, the “large fragments” of the Pol I DNA polymerases from …
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Male genital tract versus blood HIV-1 compartmentalization and selection: the first step of the transmission bottleneck?
… artificial recombination. A high fidelity Taq polymerase was also used to minimize base-substitution errors. An average of 10 clones were isolated per compartment. Pseudoviruses were then constructed from the full-length envelope clones from blood and semen. The sensitivities of these …