Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Tangential flow filtration"”.
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Tangential Flow Filtration for the Purification of Lucentis
… protein. This group has been working on the tangential flow filtration (TFF) portion of the project, which will be utilized multiple times throughout the separation and purification process. The primary purpose of tangential flow filtration is to exchange buffer solution. Exchanging buffer …
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Quantitative assessment of methods for bacterial and viral purification and concentration
… the applicability of syringe filters and a tangential flow filtration device for the purification and concentration of bacteria and virus samples, respectively. Furthermore, automation of such systems were explored. We developed a fully automated method for double filter filtration to enable …
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Performance and Fouling during Bioreactor Harvesting
<p>Tangential flow filtration has many advantages for bioreactor harvesting as the permeate could be introduced directly to the subsequent capture step, the process is easy to scale up, and fouling of the filter is limited by the cross flow. However, membrane fouling has limited its widespread use. …
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Optimization of Downstream Purification of Lucentis
… (TOSOH Bioscience) resins followed by a 30 kDa tangential flow filtration (TFF) cassette (Pall Corp). Alternative case 1 consisted of a multimodal resin (hydrophobic and cation exchange) followed by a TFF unit, hydrophobic interaction exchange resin column, and another TFF unit. This specific …
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Manufacturing and engineering of therapeutic extracellular vesicles
… standard for purifying EVs, we found that ultrafiltration-specifically tangential-flow filtration-is a more economical and scalable alternative, and we experimentally determined its utility for scaling up EV purification. For quality control, we established a suite of potency assays to measure …
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Formulation of targeted drug-loaded nanoparticles for lung cancer therapy
… fabrication characteristics such as total flow rate (TFR) and aqueous-to-lipid flow rate ratio. Selected stable liposomal formulations were then subjected to entrapment of cisplatin and irinotecan, filter centrifugation and tangential flow filtration (TFF) methods, scaleup fabrication …
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CATCHMENT SCALE MONITORING AND MODELLING OF PHOSPHORUS USING FLOW INJECTION ANALYSIS AND AN EXPORT COEFFICIENT MODEL
… the development and deployment of a portable flow injection (FI) monitor for phosphorus determination in the River Frome. The automated monitor, incorporating solenoid micropumps and switching valves, a miniature CCD spectrometer, a graphical programming environment and a tangential flow …
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Engineering layer-by-layer nanoparticles for the targeted delivery of therapeutics to ovarian cancer
… and scale. Chapter 2 describes how modern tangential flow filtration significantly improves throughput and scalability in colloidal LbL assembly. Chapter 3 implements this improved synthetic approach to generate a small library of LbL NPs that screen for tumor-targeting properties on …
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Characterization of Uranium and Rare Earth Element Mobility and Attenuation Downstream of Decommissioned Tailings Impoundments at the Bicroft Mine near Bancroft, Ontario
… were analyzed using various methods, including tangential flow filtration (TFF), ICP-ES/MS, scanning electron microscopy, and synchrotron techniques (bulk and µXANES, µXRF, and µXRD). The results show that Fe- and Mn-oxyhydroxides, goethite, and birnessite are the main mineral hosts for U and …
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Microfluidics technology for the formulation design and production of liposomal products
… has shown microfluidics parameters such as the flow rate ratio (FRR) adopted in the microfluidic process is important, there is a lack of defined operating parameters and designed space for liposome production. To this end, this thesis focuses on microfluidics technology for the production of a …
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Drug nanosizing using microfluidic reactors. Development, characterisation and evaluation of corticosteroids nano-sized particles for optimised drug delivery.
… drug particle size (as output). The antisolvent flow rate was found to have the major role in directing drug particle size. Based on these successful findings, the potential of preparing pharmaceutical nanosuspensions using microfluidic reactors was researched. A hydrocortisone (HC) …
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Quality by design for the downstream processing of biopharmaceuticals
… downstream purification of biopharmaceuticals, tangential flow filtration (TFF) is probably the second most common unit operation in the manufacture of these products. To determine how QbD can be applied to TFF a case study is developed in applying QbD techniques and mechanistic understanding to …
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DEVELOPMENT OF CRISPR-CAS IMMOBILIZATION SRATEGIES FOR THE FABRICATION OF A BIOSENSING PLATFORM
… for the immobilization on the surface of tangential flow filtration (TFF) membranes and on magnetic silica beads. Extensive studies were conducted on the method of introducing NTA onto the surface, as well as on the type of cation used for NTA activation. This approach resulted in optimal …
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Investigating alternative delivery systems for self-Amplifying RNA vaccines
… manufacturing method. Furthermore, dialysis, tangential flow filtration (TFF) and size exclusion chromatography (SEC) have been tested as purification methods and compared in terms of the ability to remove both residual organic solvent and unloaded protein from samples without altering …