Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 293 for “"Tandem Mass Spectrometry"”.
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Multifactorial modeling of ion abundance in tandem mass spectrometry experiments
Restriction data tranferred 2014-07-01T11:35:49-05:00 Original Data Group with Access Administrator Release Date: 2014-09-18 16:27:16 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system
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Determination of peptide and protein structure by tandem mass spectrometry
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1988.
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Bioinformatics of High Throughput Proteomics Using Tandem Mass Spectrometry of Intact Proteins
Top down mass spectrometry is a unique approach to the problem of identifying and characterizing proteins with a DNA-predicted sequence. It would be desirable to move top down mass spectrometry in to the 'omic' sciences by developing a high throughput form that could be used to simultaneously …
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Development of liquid chromatography with tandem mass spectrometry method for measurement of sepsis biomarkers
… faster results and detection of biomarker using mass spectrometry (MS) technique emerges as an appealing sepsis diagnostic tool. MS-based assay enables accurate measurement of biomarkers in serum and offers multiplexing capability. Measurements of biomarkers aid clinicians in their decision …
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Measuring steroids from dried blood spots using tandem mass spectrometry to diagnose congenital adrenal hyperplasia
… seven steroids using liquid chromatography mass spectrometry (LC-MS/MS) in DBS. An optimized DBS sample preparation method was employed without the need of derivatization. A LC-MS/MS assay was developed and optimized using reverse phase-ultra performance liquid chromatography (RP-UPLC) …
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Evaluation of database search programs for accurate detection of neuropeptides in tandem mass spectrometry experiments
Programs to identify proteins in tandem mass spectrometry experiments are not optimized to identify neuropeptides and other peptides resulting from the processing of prohormones. This is due to the unique characteristics of neuropeptides including release after complex processing of prohormones, …
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Identification of CaMK-II Protein Targets in Tissue Culture and Zebrafish Embryos using Tandem Mass Spectrometry
… lines in culture and used in conjunction with tandem mass spectrometry to identify binding partners, such as β-actin, tropomodulin-3 and Fli-I as well as novel, putative substrates, such as the tumor suppressor protein 53 (p53). This approach was subsequently applied to zebrafish embryos where …
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Determination of disulfide bond connecting patterns via tandem mass spectrometry (MSn) and biomolecular ion/radical reactions
… chromatography (HPLC) and then analyze them via tandem mass spectrometry (MS<sup>n</sup>). Although effective, the enzymatic digestion approach can be expensive, time consuming, nonspecific (some enzymes have a broad range of specificity), and sometimes initiate disulfide bond scrambling. The …
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Proteomic Identification of Hemp (Cannabis sativa) Seeds by Tandem Mass Spectrometry and Evaluation of Cytotoxic Activity
<p>Hemp (<em>Cannabis sativa</em>) has a long history of use globally as a source of traditional medicine, spiritual enlightenment, and recreational enjoyment. While it was commonly consumed for both medical and recreational purposes in the United States, it was classified as a Schedule 1 drug in …
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Liquid Chromatography -- Tandem Mass Spectrometry Techniques for Drugs of Abuse as Applied to Bacterial and Forensic Toxicology
… a novel liquid chromatography triple quadrupole tandem mass spectrometry method was developed for diazepam, flunitrazepam, and metabolites in Reinforced Clostridial Medium. Reinforced Clostridial Medium is a complex matrix designed to provide the nutrients necessary to promote growth of bacterial …
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Analysis of Illicit Drugs, Their Metabolites and Other Micropollutants in Water by Liquid Chromatography Tandem Mass Spectrometry
… this project is to develop liquid chromatography tandem mass spectrometry (LC/MS/MS) methods for the analysis of different, novel micropollutants in waste water and natural water. Micropollutants are compounds that can exert adverse environmental and human health effects at low concentrations. …
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High performance liquid chromatography-tandem mass spectrometry of pharmaceuticals and personal care products in environmental and biological matrices
… very low concentrations, liquid chromatography-tandem mass spectrometry (LCMS/ MS) has emerged as the new technique of choice. This work describes methods for screening and quantification of various pharmaceutical and illicit drug residues in solid and liquid environmental and biological …
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STRUCTURAL ELUCIDATION OF DEPROTONATED ANALYTES VIA TANDEM MASS SPECTROMETRY BASED ON ION-MOLECULE REACTIONS AND COLLISION-ACTIVATED DISSOCIATION
Mass Spectrometry has emerged as a powerful analytical tool for the characterization of unknown molecules. Molecular weight information and chemical formulas of the unknowns can be derived by measuring the m/z value of the ionized analyte. In addition, structural information can be obtained via …
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Quantitation of enteroendocrine hormones as metabolic disease biomarkers in human biological matrices by liquid chromatography tandem mass spectrometry
… explored in research. Liquid Chromatography Mass Spectrometry (LC-MS/MS) methods are becoming an alternative approach and analytical technique combines the physical separation abilities of liquid chromatography with specific mass analysis of mass spectrometry. The methods described in this …
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The Use of Liquid Chromatography Tandem Mass Spectrometry to Improve Newborn Screening for Congenital Adrenal Hyperplasia in New Zealand
… steroid profile test using liquid chromatography-tandem mass spectrometry (LCMSMS) and secondly, to identify novel bloodspot markers that may improve screening sensitivity for CAH. In a prospective study, the existing second-tier screening immunoassay was replaced with a LCMSMS test incorporating …
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Development of Novel Liquid Chromatography-Electrospray Tandem Mass Spectrometry Approaches for the Structural Characterization of Brevetoxins Including in vitro Metabolites
… analyzed by liquid chromatography-electrospray mass spectrometry (LC-ES-MS). Two metabolites were found for brevetoxin-1: brevetoxin-1-M1 (MW 900 Da), formed by converting one double bond in the E or F ring into a diol; and brevetoxin-1-M2 (MW 884 Da), a hydrolysis product of brevetoxin-1 …
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Comparing diene derivatisation methods of dry blood spot samples for vitamin D metabolites quantification by liquid chromatography-tandem mass spectrometry
… samples (DBS) using the liquid chromatography tandem-mass spectrometry (LC-MS/MS) analytical technique. This manuscript describes in detail the development and validation of an analytical methodology, highlighting the role derivatisation and mass spectrometry plays in the structural …
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The detection of drugs of abuse in biological matrices using enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry
The aim of this study was to investigate the potential use of ELISA and LC-MS-MS in combination and as individual techniques, for the detection of drugs of abuse in biological matrices. Overall the LC-MS-MS method showed good correlation results for opiates compared to the GC-MS method. 6-MAM was …
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