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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"TUNEL assay"”.
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VIABILITY OF CERIUM OXIDE NANOPARTICLES AS A RADIOPROTECTOR AGAINST PROTON RADIATION FOR NON-MALIGNANT CELLS
… measured with the Microculture Tetrazolium (MTT) assay 24 and 48 hours post irradiation. DNA damage was determined using the Terminal Uridine Nick-End Labeling (TUNEL) assay. While showing promise for potential radioprotection of non-malignant cells using both assays, the MTT assay results were …
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The Mechanism and Effects of TNF-α on Apoptosis in Auditory Cell Line
… (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay, assay of mitochondrial membrane potential (MMP) and electron microscopy were used to show that TNF-α could induce apoptosis. Western blot was used to measure iNOS expression and Mitogen-activated protein kinase (MAPK) pathway. Flow …
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The effect of propofol on α‐amino‐5‐methyl‐4‐isoxazole propionate(AMPA) induced neurotoxicity in rat mixed cortical cultures
… (TdT)-mediated dUTP nick-end-labeling (TUNEL) assay. The effect of propofol on AMPA mediated intracellular calcium increase was measured with calcium-sensitive fluorochrome calcium green-5N-acetoxymethylester with confocal laser scanning microscopy. Statistical analysis was done by …
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Dysregulated Apoptosis in Teratogen-Induced Neural Tube Defects in Mice
… Terminal Transferase dUTP Nick End Labeling (TUNEL) assay and gene expression changes by RT2 Profiler PCR arrays. Additionally, changes in the expression of key signal transduction pathway genes that play a role in development were determined. Increased apoptosis, suggesting involvement in VA …
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The Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement
… 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.</p> <p>Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a …
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Microcapsule drug delivery device for treatment of glioblastoma multiforme
… transferase dUTP nick end labeling (TUNEL) assay. Results showed that localized delivery of chemotherapeutics from microcapsule devices is capable of prolonging animal survival and may offer an alternative to the harsh side-effects and low response rates inherent to systemic drug …
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Investigations on the effects of Typha capensis on male reproductive functions
… and viability (determined by means of MTT assay). Part 2: The crude extract HPLC profiles were obtained by preparing the extracts for different seasons (Autumn, Winter, Spring, Summer). TM3-Leydig cells, LNCaP cells and PWR-1E cells incubated with different concentrations T. capensis …
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In vitro studies on genotoxicity and gene expression in spermatogenic cells: mechanisms and assay development
… damage and apoptosis were measured by Comet and TUNEL assay respectively. Up-regulation of expression of the transcription factors Tbpl1, FHL5 and Gtf2a1l that are important post-meiotically, were examined using RT- PCR and qPCR. Protein production was evaluated using Western blotting. Tbpl1, …
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The Effect of Embryonic Alcohol Exposure on Brain Function and Behavior in Zebrafish Strains
… apoptosis and have adapted the labeling TUNEL assay, for zebrafish. I found that mild alcohol exposure during development results in an increase in apoptosis and that these early responses result in long-lasting changes in neuronal markers and number of cells in specific brain areas. I …
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Ischemic Preconditioning Protects Adult Rat Cardiomyocytes Against Necrosis but not Apoptosis, via Activation of PKG
… blue exclusion and apoptosis was assessed by TUNEL assay. IPC reduced necrosis as shown by significant decrease in trypan blue positive cells as compared to virgin non-preconditioned myocytes subjected to SI and RO alone (p<.01). Similarly, the number of TUNEL positive myocytes following SI …
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The effects of thymic hormones on the proliferation of human myelogenous leukemia cells
… The results of morphological analyses and the TUNEL assay demonstrate that TF5 does not induce apoptosis in HL-60 cells, suggesting that the mechanism of inhibition involves cytostasis. Further purification of TF5 by gel filtration chromatography produces a biologically active factor of …
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The Effects of PPAR-γ Activators on the Growth and Apoptosis of Melanocytes
… and skin. Apoptosis of cells were detected by TUNEL assay and flow cytometry. The expression of caspase-3 and extracellular signal regulated kinase in melanocytes was measured by Western blot analysis. RESULTS: All three PPAR-γ activators, ciglitazone, troglitazone, and 15d-PGJ₂, inhibited the …
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Treatment of Aortic Heart Valve Conduit with Glutamine and Heat Shock as a Means to Deter the Constituent Cellular Population From Becoming Apoptotic
… was assessed utilizing enzyme-linked immunoassay; the presence of caspase 3 was assessed utilizing immunohistochemistry and histomorphometry; and the presence of compromised DNA was assessed utilizing the TUNEL assay and histomorphometry. It was found that treatment of allograft heart valve …
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Notch Signaling Is Important in the Survival, Proliferation, and Self-Renewal of the Putative Breast Cancer Stem Cell Population
… after treatment with one GSI, MRK003, by TUNEL assay and Annexin V - propidum iodide staining. The mechanism of apoptosis was explored utilizing a RT-qPCR based approach as well as several kinase inhibitors including those that inhibit c-Jun N-terminal kinase and the p38 mitogen activated …
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PRIMING CARDIOVASCULAR STEM CELLS FOR TRANSPLANTATION USING SHORT-TERM HYPOXIA
… Cellular function was evaluated by the use of a TUNEL assay and Transwell® invasion assay.</p> <p>Hypoxia-mediated alterations of a genetic or functional nature in Isl-1+ c-Kit+ human cardiac progenitors are clearly age-dependent. Although both age groups accrued benefit, the neonatal progenitors …
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The Antitumor Agent, Arglabin-DMA, Preferentially Induces Apoptosis in Human Colon Tumor Cells
… its antitumor effects. Gel electrophoresis, TUNEL assay, Annexin V assay, apoptosis dye-uptake assay, and morphological criteria were used to characterize apoptosis. Adherent cells and freely floating detached cells in Arglabin-DMA treatment were treated as two distinct populations We …
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Molecular Basis of Calcicole-Calcifuge Physiology.
… markedly in Glenisla plants grown at 30 mM Ca2+. TUNEL assay determined the occurrence of apoptosis in the same Glenisla roots. Also, ten repressed Calcium-responsive genes in Cal-0 (Cal-CRGs) at high Ca2+ were investigated in all the six ecotypes using Real time RT-PCR, demonstrating their …
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Unique localization of protein kinase C isozymes in T51B rat liver cells and their role in proliferation and apoptosis.
… by flow cytometry, Hoechst staining and TUNEL assay. Furthermore, flow cytometric analyses indicate that as the cells approach confluence, the percentage of apoptotic cells increases with a corresponding decrease in the percentage of the G2 cell population. The role of PKC isozymes in the …
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Corneal fibrosis therapies and investigations in the canine corna in vitro
… A series of dose-dependent and time-dependent assays with lisinopril (1-50 µM) and fasudil (1-10 nM) were performed. qRT-PCR, immunofluorescence (IF) staining, cell viability assay, cell proliferation assay, LIVE/DEAD Viability/Cytotoxicity assay, TUNEL assay, and total cell count were …
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Protective effects of regular exercise and Korea red ginseng against high-fat diet-increased renal injury in ᴅ-galactose-induced aging rats
… was examined by enzyme-linked immunosorbent assay (ELISA), and KRG (200 mg/kg/day) was given to GAL plus HFD-induced aging rats for 9 weeks. Immunohistochemical staining for 8-OHdG (a specific marker of oxidative DNA damage) and CMLs (a marker of both glycation and lipoxidation reactions) …
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