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Showing 1 to 20 of 99 for “"TUNEL"”.
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Estado tenso-deformacional alrededor de un tunel excavado en un medio elastoplástico con especial consideración de la influencia del proceso constructivo
Estado tenso-deformacional alrededor de un tunel excavado en un medio elastoplástico con especial consideración de la influencia del proceso constructiv
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Optimalizace geotechnického průzkumu a monitoringu při navrhování a provádění podzemních staveb
… z konkrétních staveb v ČR (Královopolský tunel, tramvajový tunel VMO Žabovřeská) a na základě autorových praktických tuzemských i zahraničních zkušeností podává návrh na jejich vzájemnou optimalizaci za účelem zkvalitnění výstupů a snížení finančních nákladů.
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중뇌동맥을 결찰한 흰쥐의 산화망간조영증강 자기공명영상(MONERI) 연구
… 10 4. 시간에 따른 신호 대 잡음비의 측정 11 5. H & E 염색 13 6. TUNEL 염색 13 7. 통계분석 14 Ⅲ. 결과 15 A. 중뇌동맥 폐색 후 흰쥐의 MONEMRI 영상소견 15 B. 뇌경색 이후 각 영역별 신호 대 잡음비의 시간에 따른 변화 25 C. 뇌손상 조직의 H&E 염색표본과 MR영상 비교 28 D. 뇌손상 조직의 TUNEL 염색표본과 MONEMRI 비교 32 Ⅳ. 고찰 35 Ⅴ. 결론 38 참고문헌 39 ABSTRACT 44
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Selective irradiation of the vascular endothelium
… 4 hrs after irradiation and stained with TUNEL to observe and score apoptotic cells in the villus lamina propria. To uniquely identify the type of cell undergoing apoptosis in the lamina propria, intestinal specimens from various mice in the TUNEL studies were sectioned and stained with …
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Ischemic Preconditioning Protects Adult Rat Cardiomyocytes Against Necrosis but not Apoptosis, via Activation of PKG
… blue exclusion and apoptosis was assessed by TUNEL assay. IPC reduced necrosis as shown by significant decrease in trypan blue positive cells as compared to virgin non-preconditioned myocytes subjected to SI and RO alone (p<.01). Similarly, the number of TUNEL positive myocytes following SI …
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The Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement
… 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.</p> <p>Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a …
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Identification of Apoptotic Regulators in the Cervix of Pregnant Mice in Association with Cervical Softening
… by specific immunohistochemistry, Western blot ,TUNEL. And then the expressions of the Bcl-2, Bax, Caspase-3, Caspase-6 were analyzed. Stromal arrangements associated with apoptosis were assessed. Apoptosis is detected significantly during late pregnancy by TUNEL. On Western blotting; Expression …
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VIABILITY OF CERIUM OXIDE NANOPARTICLES AS A RADIOPROTECTOR AGAINST PROTON RADIATION FOR NON-MALIGNANT CELLS
… using the Terminal Uridine Nick-End Labeling (TUNEL) assay. While showing promise for potential radioprotection of non-malignant cells using both assays, the MTT assay results were less conclusive and require further research. The TUNEL assay results are preliminary but show that CNPs reduce …
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The Mechanism and Effects of TNF-α on Apoptosis in Auditory Cell Line
… (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay, assay of mitochondrial membrane potential (MMP) and electron microscopy were used to show that TNF-α could induce apoptosis. Western blot was used to measure iNOS expression and Mitogen-activated protein kinase (MAPK) pathway. Flow …
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Cellular pathology and apoptosis in experimental and human acute and chronic compressive myelopathy.
… inflammatory infiltrate. Glial positivity using TUNEL occurred at 24 hours and 1 week post-injury. PARP, DNA-PKcs and AIF immunopositivity occurred in glia at, above and below the site. APP immunopositivity was present in axonal swellings. In human chronic compression, axonal swellings, loss of …
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The role of EGFR, HER 2-4, heregulin and downstream signalling in prostate adenocarcinoma
… staining for HRG, KI67 (proliferation), TUNEL (apoptosis) was performed on pilot study specimens. Further IHC for EGFR, HER2, HER3, HER4, HRG, KI67 and TUNEL was performed on HSPC tissue microarrays. Correlations between target protein expression and the outcomes time to biochemical …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… transferase dUTP nick-end labelling (TUNEL) and 5-ethynyl-2-deoxyuridine (EdU) assay. Results: Undifferentiated mESCs were positive for pluripotent transcription factors Nanog and Oct3/4, whereas the cardiac differentiated mESCs were positive for cardiac proteins troponin T, α-actinin …
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The Effects of Growth Hormone in the Inner Ear of Zebrafish (<i>Danio rerio</i>) during Hair Cell Regeneration
… in fish inner ear end organs were examined using TUNEL-labeling. Cell proliferation was measured by BrdU incorporation assay. Hair cell regeneration was determined by phalloidin-labeling to allow visualization of hair cell stereociliary bundles. After GH injection, the numbers of TUNEL-labeled …
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Treatment of Aortic Heart Valve Conduit with Glutamine and Heat Shock as a Means to Deter the Constituent Cellular Population From Becoming Apoptotic
… of compromised DNA was assessed utilizing the TUNEL assay and histomorphometry. It was found that treatment of allograft heart valve conduit tissue at 37°C plus glutamine lead to a statistically significant increase in heat shock protein 70 concentration, maintenance of the mitochondrial …
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Cardioprotective Effect of Onion Extract against Ischemic Injury In vitro and In vivo
… c release, caspase-3 activation, and increase in TUNEL-positive cells. Onion extract also inhibited elevation of ROS generation induced by hypoxia. From inhibitory effects of onion extract and a well-known antioxidant trolox on hypoxia-induced decrease in mitochondrial membrane potential. it is …
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Vitrification of Bovine Oocytes
… transferase dUTP nick end labeling (TUNEL) and caspase assays. Mouse zygotes were exposed to increasing concentrations of ethylene glycol (EG), Ficoll-70 and sucrose in phosphate buffered saline (PBS) for vitrification on nylon mesh and plunged into liquid nitrogen. Warming resulted …
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Characterization of Apoptotic Cells in Equine Proximal Suspensory Desmitis
… transferase mediated dUTP nick end labeling (TUNEL) technique. Histological changes in the abnormal ligaments included mineralization, fibroplasias, neovascularization, collagen degeneration, and significant architecture disruption in 2 ligaments. There was a trend for increased apoptosis in …
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The effect of propofol on α‐amino‐5‐methyl‐4‐isoxazole propionate(AMPA) induced neurotoxicity in rat mixed cortical cultures
… (TdT)-mediated dUTP nick-end-labeling (TUNEL) assay. The effect of propofol on AMPA mediated intracellular calcium increase was measured with calcium-sensitive fluorochrome calcium green-5N-acetoxymethylester with confocal laser scanning microscopy. Statistical analysis was done by …
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Dysregulated Apoptosis in Teratogen-Induced Neural Tube Defects in Mice
… Terminal Transferase dUTP Nick End Labeling (TUNEL) assay and gene expression changes by RT2 Profiler PCR arrays. Additionally, changes in the expression of key signal transduction pathway genes that play a role in development were determined. Increased apoptosis, suggesting involvement in VA …
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Glucose-Induced Apoptosis of Schwann Cells In vitro
… transferase mediated dUTP nick end labeling (TUNEL) method. Approximately 50% of the Schwann cells exposed to elevated glucose levels were apoptotic after 24 hours of incubation, and apoptosis of Schwann cells increased with increasing time in culture to 71% on day six in culture. This finding …
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