Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"TSG101"”.
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Endosomal Sorting Complex Required for Transport (ESCRT): Investigation of the expression and functional roles in neurodegenerative diseases
… to age matched brains. The expression of TSG101 is markedly decreased in MND brains, spinal cord, and AD brains in comparison with control. Also, the level of VPS37A&B in MND is lower than in control brains and spinal cord but unchanged in AD brains. While the expression of CHMP2B …
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Investigation of Cancer Stem Cells (CSCs)-derived exosomes and their influence on the tumor microenvironment
… that, in addition to well known markers, such as Tsg101 and CD81, LCSC-derived exosomes are enriched in CD133 protein. Immunoprecipitation (IP) experiments revealed that by immunoprecipitating the culture SN of CD133 expressing cells with an antibody against CD133 protein, typical exosomal …
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Development of a multi-stage purification process for serum-derived exosomes and evaluation of their regenerative capacity
… vesicles and exosomal markers (CD9, CD81 and TSG101). These isolates allowed for cellular uptake of a range of fluorescent labels and enhanced cellular fibroblast proliferation and endothelial sprout formation in a 3D spheroid-based angiogenesis assay. Further to this, functionality was shown …
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Genomic signatures of recent selection at HIV host susceptibility genes in a Central African human population
… showed a strong signal of selection at the ARG TSG101. Pygmies have also been found to carry genotypes protective against HIV-1 for the ARGs CCR2, CCL3L1 and CCR5.
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Role of Ubiquitin and Ubiquitin Ligases in Retroviral Budding
… interaction partners: PT/SAP domains bind Tsg101, LxxLF or YPXL domains bind ALIX, and PPxY domains bind Nedd4-like HECT ligases, a class of ubiquitin ligase enzymes. The functional link between HECT ligases and the VPS pathway has not been defined, but multiple lines of evidence suggest …
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Isolation und Charakterisierung extrazellulärer Vesikel aus humanen CD8+- und γδ-T-Lymphozyten
… CD9 und CD81 sowie HSP70, Syntenin-1, TSG101 und ALIX. Insgesamt ergab sich ein weitgehend homogenes Spektrum an Proteinen in EV beider T-Zellpopulationen. Die Gen-Ontologie-Bewertung ergab eine deutliche Anreicherung von Proteinen, die mit extrazellulären Vesikeln und deren Biogenese, …
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Structural and functional studies of human ADAM 12 in myoblast fusion and Ebola virus VP40 in assembly
… ligase(Nedd4) and to an inactive E2 enzyme (Tsg101),both of which are here shown to interact with VP40 <i>in vitro</i>. These interactions are believed to improve the budding efficiency.
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Caractérisation morphologique et moléculaire des vésicules extracellulaires libérées par des cellules musculaires primaires humaines provenant d'individus sains et les personnes atteintes de diabète de type 2
… par Western blot pour les marqueurs vésiculaires TSG101, CD63, CD81. Parmi les méthodes d’isolement testées, ExoQuick et EasySep se sont révélés être plus performants en termes de rendement et de pureté avec respectivement 4,2.10¹⁰ particules/mL et 4,3.1013 particules/mL et une absence de …
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A phylogenetic study of ubiquitin conjugating enzymes and structural analysis of human UBE2J proteins
… the human UBE2L proteins and secondly the TSG101 UBC like proteins, both of which branches do not appear to have any yeast orthologues.<br/><br/>2) The UBC phylogenetic study was also used to identify the previously unknown UBC active site of the <i>Drosophila </i>transcription factor, …
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Isolation and characterisation of extracellular vesicles in baculovirus infection of insect cells
… then analysed. Homologs of sEV protein markers TSG101 and syntenin-1 were identified. In sEVs from AcΔp6.9-transfected cells proteins from the mitochondrion, proteasome complex, ribosome, and involved in ubiquitination were enriched compared to sEVs from mock-transfected cells. These were …
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Differential expression of proteins involved in sEV biogenesis and secretion in Parkinson’s disease and Dementia with Lewy bodies
… for both patients with PD and DLB. Levels of TSG101 were also significantly decreased in the substantia nigra of PD patient samples compared to the healthy control group. Given the key roles of Alix and TSG101 in sEV biogenesis, cargo sorting into sEVs, and maintenance of the ALP, and …
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Uptake of Small EVs by Skeletal Muscle Myoblasts In Vitro and Subsequent Myogenic Response to Cargo
… blot analysis further confirmed the presence of TSG101, Alix, and CD81, and the absence of the endoplasmic reticulum protein calnexin, indicating minimal cellular contamination. Endogenous miR-206 expression was assessed in proliferating C2C12 cells and in their released small EVs before cargo …
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Determinanten und Mechanismen der foamyviralen Partikelfreisetzung
… Interaktion des PFV Gag PSAP L-Domän Motivs mit TSG101, einer frühen Komponente der ESCRT-Komplexe, verbindet PFV mit dem VPS-Sortierungsweg der Zelle. Die besondere Fähigkeit des FV Env-Proteins zur Freisetzung von SVPs wurde bereits vor einiger Zeit entdeckt, dennoch war bisher nichts über die …
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Investigation and application of substrate tolerant thioether forming enzymes
… ubiquitin E2 variant (UEV) domain of the human TSG101 protein, which is essential for HIV virus budding from infected cells. A detailed in vitro characterization was performed to identify the binding site of XY3-3 using fluorescence polarization. XY3-3 competes with full length p6 protein for …
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Glycoprotein M and ESCRT in herpes simplex virus type 1 assembly
… ESCRT during HSV-1 infection is independent of TSG101 and/or ALIX. Data presented in this thesis demonstrate that CHMP4A/B/C proteins of the ESCRT-III complex are specifically crucial for HSV-1 secondary envelopment. Simultaneous depletion of CHMP4A/B/C proteins significantly inhibited HSV-1 …
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Role of two genes, CACNA1D and CADM1, with common or rare mutations in aldosterone producing adenomas of the adrenal.
… on plasma membrane in the CADM1 silenced cells. TSG101, a protein involved in lysosomal degradation of Cx43 was downregulated in the absence of CADM1 and possibly the mechanism for increased Cx43 expression. Also, immunostaining of adrenal sections showed internalised para-nuclear staining …