Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"TRIM28"”.
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Trim28 Interacts With Ezh2 to Regulate Its Activity
<p>In this dissertation, I discovered that TRIM28 interacts with EZH2 and regulates its activity in breast cancer cells. EZH2, the major H3K27 tri-methyltransferase, is over expressed in multiple subtypes of breast cancer; however, whether EZH2 plays an active role in breast cancer progression or …
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Characterising the interaction between TRIM28 and members of the Krab-ZFP family in transcription silencing
… during early embryogenesis, largely through TRIM28-mediated transcription repression. TRIM28 (also known as KAP1 or TIF1β) functions as the co-repressor protein for the largest family of transcription factors, namely the Krüppel-associated box-containing zinc finger proteins (Krab-ZFPs). …
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The Cancer Specific Ubiquitin Ligase MAGE-A3/6-TRIM28 Drives Tumorigenesis by Ubiquitination and Proteasomal Degradation of AMPK
… binds and regulates the E3 ubiquitin ligase TRIM28, I investigated its biochemical role in cancer. I used unbiased methods to identify 19 novel substrates of MAGE-A3/6-TRIM28, including the known tumor suppressor AMPK. Ubiquitination of AMPK by MAGE-A3/6-TRIM28 induces its proteasomal …
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A TRIM28 centric strategy to efficiently uncover the contribution of KZFPs to the evolution of gene regulatory networks
… TEs in a sequence specific manner and recruit TRIM28 which interacts with other proteins to add H3K9me3, inducing silencing-associated heterochromatin. Interestingly, it was discovered following a large-scale survey of their binding sites that most human KZFPs target evolutionary conserved …
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Transposable Elements in Neural Progenitor Cells
… progenitor cells. In this thesis, I identify TRIM28 as key regulator of certain groups of transposable elements in mouse and human neural progenitor cells. This feature is unique compared to other somatic tissues, where DNA-methylation is prevalent. Here I demonstrate, that transposable …
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Using a novel large-scale epigenetic analysis method to uncover the cis-regulatory potential of individual transposable elements
… them. This is achieved by recruitment of TRIM28 and the deposition of the repressing H3K9me3 histone modification, which leads to formation of heterochromatin and restricts accessibility of transcription factors. Similarly to TEs, it was initially hypothesised that this silencing mechanism …
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Functional Analysis of KAP1 Requirements for HIV-1 Transcription and Latency Reactivation
… (KRAB)-associated protein 1 (KAP1: also named TRIM28 or TIF1β). While initial studies have revealed KAP1 to be a positive regulator of latency reversal in transformed and primary CD4+ T cells, subsequent studies have proposed KAP1 to be a repressor required for latency maintenance. Given this …
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Transposable elements and the regulatory logic of hematopoietic differentiation
… EHMT1 or transcriptional co-repressor TRIM28 induced NK-fated progenitors that ultimately generated NK cells with diverse effector properties. We further leveraged TEs by repurposing the packaging function of the MLV gag polyprotein to create a non-destructive reporter of the …
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Molecular mechanism of KAP1-dependent transcriptional silencing
… KRAB-associated protein 1 (KAP1, also known as TRIM28 or TIF1β). Following its recruitment to retrotransposons by sequence-specific KRAB domain-containing zinc finger proteins (KRAB-ZFPs), KAP1 induces the assembly of an epigenetic silencing complex, with chromatin remodeling activities that …
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Identification and characterisation of KZFPs directly regulated by p53
… ZNF79 demonstrates an unusual intermediate TRIM28 binding status and a unique nucleolar localisation. In contrast, ZNF561 resembles a canonical KZFP but possesses intriguing characteristics given that its expression is induced by p53. First, its binding profile is enriched for specific LTR10 …