Global ETD Search
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Showing 1 to 20 of 23 for “"TRF2"”.
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Mechanisms of Telomere End Protection by TRF2
… telomeres. The six subunits of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from activating various DNA damage response (DDR) signaling and double-strand break repair …
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Functional Analysis of the Human Telomeric Protein TRF2
<p>TTAGGG Repeat Binding Factor 2 (TRF2) is a ubiquitous human telomeric protein present at all telomeres throughout the cell cycle. TRF2 has been proposed remodel telomeres into large lasso-like structures named t-loops. Removal of TRF2 from telomeres by expression of a dominant negative allele of …
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The Apollo Nuclease Binds To TRF2 and Protects
… in complex with the telomere binding proteins TRF2/Rap1. Due to its low abundance at telomeres, we consider Apollo to be a telomere accessory factor, and not part of the core telomere protein complex, shelterin. Apollo localizes to telomeres by interacting directly with the TRFH domain of TRF2. …
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A Novel Phosphorylation Site in the Telomeric Protein TRF2 is Regulated by the ATR Kinase and Plays a Role in Relieving Replication Stress at the Telomere
… We report that the human shelterin component, TRF2, overexpressed in 293T cells, is phosphorylated on serine 368 (S368) in a caffeine and wortmannin sensitive manner. Phosphorylation is induced by hydroxyurea (HU) and ultraviolet (UV) radiation but not Ionizing Radiation (IR). Knockdown studies …
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MULTI-ORGAN FAILURE DRIVEN BY TELOMERE UNCAPPING
… breaks, a function mostly dependent on TRF2. Loss of TRF2 results in telomere uncapping, activation of the DNA damage response, and chromosomal end-to-end fusions, with dramatic consequences for genome integrity and cell fate. The impact of TRF2 depletion is context-dependent, causing …
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Further Insight into the Two Functions of Telomeres: Determining the Role of Tankyrase1 in Telomere Length Regulation and Tin2 in Telomere Protection
… telomeric DNA-binding proteins TRF1 and TRF2 independently or simultaneously, acting as a bridge linking TRF1 and TRF2 to TPP1 and POT1, the single-stranded telomeric DNA-binding protein. To gain further insight into the function of the TRF2-TIN2 complex, we created a TRF2 mutant that no …
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Testing the T-loop Model of Telomeric End Protection
… anchor points of shelterin is a protein known as TRF2. TRF2 is necessary to protect telomeres from becoming fused by non-homologous end-joining, and from one of the two main DNA damage signaling pathways, in this case the one driven by ATM and CHK2. It is thought to do this by rearranging the very …
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Functional analyses of hTel2 and TRF2 deltaB: Insights Into the Role of a New DNA Damage Pathway and Homologous Recombination in Mammalian Telomere Function
… its multiple localizations and phenotypes. TRF2 protects telomeres from non-homologous end joining (NHEJ), possibly through the formation of a t-loop structure. A mutant allele of TRF2, TRF2AB, retained the ability to suppress NHEJ but induced dramatic deletions of telomeric DNA. This …
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Distinct functions of POT1 at telomeres.
… binds the telomeric dsDNA‐binding protein TRF2 and participates in heterodimeric complex with the protein TPP1. Inhibition of POT1 induces a robust DNA damage response at telomeres and deregulation of telomere length homeostasis, indicating that POT1 is important in maintaining telomere …
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Distinct Functions of POT1 in Telomere Protection and Length Regulation
… double-stranded DNA (dsDNA) binding protein TRF2. I sought to determine which of these functions--ssDNA, TPP1, or TRF2 binding--was required for POT1-mediated telomere localization, protection, and length regulation. Using separation-of-function POT1 mutants deficient in one of POT1's three …
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Functional Characterization of Plasmodium falciparum TATA-box binding-like Protein (PfTLP)
… Bioinformatic analysis suggested that PfTLP is a TRF2-type TBP-like protein. However, PfTLP differs in several aspects from previously characterized TRF2-type proteins. These differences are thought to be Plasmodium specific adaptations to the parasite’s intricate life cycle and AT-rich genome. …
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Characterization of DNA Binding Activities at Vertebrate Telomeres
… bound in vivo by two related factors, TRF1 and TRF2, which both contain one Myb-type repeat. We have conducted a detailed analysis of the DNA binding mode of TRF1 and shown that it binds DNA as a dimer by contacting a bipartite DNA site with each Myb domain. The relative orientation and spacing …
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Funktionelle Charakterisierung der humanen Proteinkinase Aurora-C in Krebszellen
… identifiziert wurde, ist das Telomerbindeprotein TRF2. Im Rahmen dieser Arbeit konnte gezeigt werden, dass die Kinase Aurora-C das Substrat TRF2 in vitro an der Position Threonin 358 spezifisch phosphorylieren kann.
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Replicative Senescence is ATM-Driven, Reversible, and Delayed by Reduced ATM Activity at Low Oxygen
… that the shortest telomeres lack su]icient TRF2 to repress ATM signaling. However, ATM-deficient cells also undergo replicative senescence, raising the question of POT1 deficiency and subsequent ATR signaling. Moreover, low oxygen culture conditions impart an extension of fibroblast …
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Investigating Mechanisms of Telomere End-Protection
… Mammalian Rap1 interacts with shelterin factor TRF2, to localize to telomeres, and human Rap1 is implicated in repressing NHEJ. Surprisingly, removal of Rap1 from telomeres revealed that mouse Rap1 was not required to inhibit NHEJ, but instead was critical for the repression of the DSB repair …
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Characterization of a Novel 53BP1-Dependent Mechanism that Promotes Non-Homologous End Joining of Deprotected Telomeres by Increasing Chromatin Mobility
… by the removal of the shelterin component, TRF2. As a consequence of TRF2 loss, deprotected chromosome ends trigger a sequence of events normally activated by the presence of DSBs. These include the instigation of ATM mediated activation of cell cycle checkpoints and the accumulation of DNA …
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Preclinical evaluation of a novel telomerase inhibitor in malignant cells of the hematopoietic system
… decapping which was associated with loss of TRF2 and increased phosphorylation of p53. Ectopic expression of hTERT revealed the close correlation of the protective role of the catalytic activity, telomere length and cellular stability. We conclude that High Dose BIBR1532 exerts a direct …
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Elucidation of Snm1B Function
… Snm1B binds to the telomere binding protein TRF2 to help protect telomeres from DNA damage during S phase. In addition, Snm1B protects DNA from agents that induce interstrand crosslinks (ICLs), lethal lesions that covalently attach the opposite strands of DNA together. To elucidate how Snm1B …
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Pathways of NHEJ at Dysfunctional Telomeres and Their Resolution
… (HR). Telomeres lacking the shelterin protein TRF2 have proven a versatile system for studying 53BP1 in DSB repair since 53BP1 protects dysfunctional telomeres from resection and promotes their mobility. Analysis of separation of function mutants of 53BP1 has identified the domain that is …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
… role of the Rapl complex, consisting of hRapl, TRF2, and the Mrell complex, in human telomere biology. A yeast two-hybrid screen was carried out using hRapl as a bait and two genes were identified. The first gene, called candidate 144 (C144), encodes a novel zinc-finger containing protein. …
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