Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"TRF1"”.
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Mechanistic Understanding of the Role of TRF1 in Telomere Replication
… are hard to replicate, and the shelterin subunit TRF1 is important for facilitating the replication of telomeric DNA. Deletion of TRF1 results in several phenotypes that are thought to be associated with telomere replication stress, including ATR signaling, fork stalling, sister telomere …
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Further Insight into the Two Functions of Telomeres: Determining the Role of Tankyrase1 in Telomere Length Regulation and Tin2 in Telomere Protection
… its interaction with the shelterin component TRF1. Tankyrase1 poly(ADP-ribosyl)ates TRF1 in vitro, and its nuclear overexpression leads to loss of TRF1 and telomere elongation, suggesting that tankyrase1 is a positive regulator of telomere length. In agreement with this proposal, we showed …
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Development of embryonic stem cells expressing endogenous levels of a fluorescent protein fused to the telomere binding protein TRF1
… expressed protein Telomere Repeat Factor 1 (TRF1) tagged with a fluorescent protein. TRF1 is a negative regulator of telomere length that binds exclusively to telomere repeats. Over-expression of the fluorescent protein fused to TRF1 has been a useful tool to track telomere movement. The foci …
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Characterization of DNA Binding Activities at Vertebrate Telomeres
… and is bound in vivo by two related factors, TRF1 and TRF2, which both contain one Myb-type repeat. We have conducted a detailed analysis of the DNA binding mode of TRF1 and shown that it binds DNA as a dimer by contacting a bipartite DNA site with each Myb domain. The relative orientation and …
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Pathways of NHEJ at Dysfunctional Telomeres and Their Resolution
… that are activated when the shelterin component TRF1 is absent. The results provide tantalizing evidence that sister telomeres can fuse via the alternative-NHEJ (a-NHEJ) pathway when replication forks reach the end of the chromosome in cells that are deficient in TRF1. To prevent chromosome …
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Characterization and consequences of Telomere replication gone awry
… binding protein Taz1 (a homologue of mammalian TRF1 & 2). In the absence of Taz1, replication forks stall when encountering telomeric sequences, leading to telomere breakage and loss. This unanticipated function of the telomeric complex suggests that telomeric sequences pose an obstacle to the …
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Mechanisms of Telomere End Protection by TRF2
… called telomeres. The six subunits of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from activating various DNA damage response (DDR) signaling and double-strand break repair …