Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"TRAP-assay"”.
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Telomerase Activity in Prostatic Fluid and Tissue as a Marker for Prostate Cancer
… of the Telomeric Repeat Amplification Protocol (TRAP) assay for the detection of telomerase activity in prostate needle biopsy specimens and freshly collected prostatic fluid. Clinical application of a newly developed PCR-ELISA telomerase assay method is also evaluated. Multiple sextant needle …
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G4-RESOLVASE 1 TIGHTLY BINDS AND CATALYTICALLY UNWINDS UNIMOLECULAR G-QUADRUPLEX STRUCTURES WITH IMPLICATIONS FOR GENE TRANSCRIPTION AND TELOMERE BIOLOGY
… Using a novel peptide nucleic acid (PNA) trap assay, we show that G4R1 catalyzes unwinding of unimolecular G4-DNA into an unstructured state. Additionally, we provide strong evidence for the presence of unimolecular G4-structures in the promoter of the Yin Yang 1 (YY1) gene and demonstrate …
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Synthesis and biophysical evaluation of thiazole orange derivatives as DNA binding ligands
… of telomerase activities was studied using the TRAP assay. The cytotoxicity of these derivatives toward three cancer cell lines was also investigated using the MTS assay. The second part of this work focuses on development of TO-based G-quadruplex DNA binding ligands that can bind to DNA via the …
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Telomerase Activity in Human Preimplantation Embryos
… the telomeric repeat amplification protocol (TRAP) assay system for increased sensitivity to allow detection of telomerase activity in the single cell of an oocyte and embryo, obtain telomerase activity levels for the oocyte through blastocyst, and finally, evaluate the use of measuring …
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Telomerase : a tumor marker in the peripheral blood of colorectal cancer patients
… a combination of approaches including; ELISPOT assay to detect viable CTL that can recognise hTERT peptides in 30 patients with CRC. The results showed that 71% of pre-operative samples and 54% post-operative samples had CTL reaction towards hTERT peptides. The second approach was to study the …
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Study of DNA damage on DNA G-quadruplexes and biophysical evaluation of the effects of modified bases (lesions) on their conformation and stability
… lesion on the human telomerase activity using TRAP assay will be discussed.</p>
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Novel Methods to Characterise Atmospherically Relevant Organic Radicals and Reactive Oxygen Species
… the gas and particle phase are presented. Spin trap molecules are used to scavenge CIs to form stable 1:1 adducts which are subsequently detected and quantified using mass spectrometry. The chemistry of CIs with spin traps is extensively investigated. The unique capability of this technique to …
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Aktivitätsmessung auf nukleinsäuremodifizierten Oberflächen
… Zellen aufweist. Die Vorteile dieses neuen Assays gegenüber älteren Methoden liegt im Verzicht auf radioaktiv-markierten Komponenten und einer deutlich verkürzten Analysezeit. Die Arbeit schliesst mit einem funktionsfähigen Nachweis der Telomeraseaktivität im Zellextrakt von gesunden und …