Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"TPP1"”.
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Structure Determination of Viscotoxin A1, Tendamistat and Tri Peptidyl peptidase-I
… Kinderkrankheit wird durch eine Mutation im tpp1-Gen ausgelöst, welches TPP1 kodiert. Kristalle der voll glukosilierten Vorstufe von TPP1 traten unter Bedingungen auf, die einen partiellen autokatalytischen Schnitt zwischen dem Prosegment und der katalystischen Domäne vermuten lassen. Die …
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Biophysical and Therapeutic Approaches to Neurodegenerative Disease: Insights From Lysosomal and RNA-Binding Protein Systems
… control mediated by tripeptidyl peptidase 1 (TPP1), and RNA‑regulated proteostasis governed by the RNA‑binding protein TDP‑43. First, I reviewed general mechanisms of proteostasis disruption, highlighting how lysosomal storage disorders and RNA‑binding proteinopathies exemplify convergent …
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Distinct Functions of POT1 in Telomere Protection and Length Regulation
… latter, POT1 heterodimerizes with the protein TPP1 to foster binding to telomeric ssDNA in vitro and binds the telomeric double-stranded DNA (dsDNA) binding protein TRF2. I sought to determine which of these functions--ssDNA, TPP1, or TRF2 binding--was required for POT1-mediated telomere …
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Biochemical and structural studies of human telomerase regulation
… to telomeres by the shelterin component TPP1 (Nandakumar et al., 2012; Sexton et al., 2012; Zhong et al., 2012), which forms a heterodimer with another shelterin component POT1 (Xin et al., 2007). After synthesizing approximately 60 telomeric repeats, telomerase is terminated by a CST …
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Distinct functions of POT1 at telomeres.
… in heterodimeric complex with the protein TPP1. Inhibition of POT1 induces a robust DNA damage response at telomeres and deregulation of telomere length homeostasis, indicating that POT1 is important in maintaining telomere stability and in regulating telomere length. The goal of my thesis …
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The Role of Pot1 in Telomere Protection and Maintenance
… analysis of POT1 and its binding partner TPP1 predict that they act interdependently. Consistent with this, we found that TPP1 is essential for the telomeric function of both mouse POT1 proteins. Single knockouts and complementation experiments revealed that POT1a and POT1b have distinct …
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Structural dynamics of telomeric overhang accessibility
… telomeric-binding proteins such as POT1 and TPP1 can sequester the overhang to regulate telomerase activity in vivo. Furthermore, existing small molecules that bind G-quadruplex have been shown to inhibit telomerase activity. However, there is little evidence on how the telomere-binding …
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On the Second Telomere Maintenance Machine: Structure, Recruitment, and Regulation of the CST–Polα/primase C-strand Fill-in Complex
… CST–Polα/primase is recruited to telomeres. POT1/TPP1 is the heterodimer in shelterin that interacts with the ss 3' overhang, and it was previously implicated in CST recruitment.I determined cryo-EM structures of POT1/TPP1 bound to CST, which revealed the molecular basis for the recruitment of …
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Further Insight into the Two Functions of Telomeres: Determining the Role of Tankyrase1 in Telomere Length Regulation and Tin2 in Telomere Protection
… acting as a bridge linking TRF1 and TRF2 to TPP1 and POT1, the single-stranded telomeric DNA-binding protein. To gain further insight into the function of the TRF2-TIN2 complex, we created a TRF2 mutant that no longer associates with TIN2. Employing protein overlay assays, we established that …
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Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… leaving a 3'-phosphate that can be removed by Tpp1, Apn1, or Apn2 forming a substrate suitable for repair by DNA polymerase δ, FEN1 and DNA ligase.</p>
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Mechanisms of Telomere End Protection by TRF2
… The six subunits of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from activating various DNA damage response (DDR) signaling and double-strand break repair pathways. The need …