Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"TISSUE BLOCKS"”.
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Implementation and Cost Analysis of Performing Molecular Assays using FFPE Tissue Blocks in the Clinical Laboratory at VA Palo Alto Health Care System
… process Formalin-Fixed Paraffin-Embedded (FFPE) tissue blocks.</p> <p>At VA Palo Alto Health Care System, FFPE samples for BRAF, KRAS and EGFR mutation detection assays are usually sent out to reference laboratories. However, with the increasing demand and cost of these tests, it would be more …
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ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY
… The expression of these proteins was observed in tissues sections from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the …
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Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas
… fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, …
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Poroviscoelastic Modeling of the Mechanical and Fluid Response of Liver Tissue in Unconfined Compression
… compression tests on unperfused and perfused tissue blocks. This data was then used in model development, leading to finite element models capable of predicting mechanical and fluid response in tissue specimens. This strategy serves as the foundation to future testing and model development, …
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Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation
… Johne's disease and positive culture of MAP from tissues provides definitive diagnosis of paratuberculosis. To determine assay sensitivity, 85 formalin-fixed paraffin-embedded (FFPE) tissues from ruminants diagnosed with Johne's disease were tested with a commercial paratuberculosis quantitative …
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Cellular and Synaptic Morphology of Golgi -Impregnated Cortical Neurons in Patients With Schizophrenia
… or antipsychotic treatments. Formalin-fixed tissue blocks from frontopolar cortex, Area 10, and primary visual cortex, Area 17, were acquired from a pool of 16 subjects with chronic schizophrenia and 22 normal comparison subjects. Golgi-impregnated pyramidal neurons from Layer 3 and Layer 5 …
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A study of the functional anatomy of the bovine cervix with special reference to the epithelium, mucus secretion and sperm transport
… included: 1. Surface staining of fixed tissue blocks in Harris' hematoxylin followed by steriomicroscopic examination, 2. Model construction from serial sections using both computer-aided and plexiglas assembly of epithelial tracings, 3. Histochemical investigation using five follicular …
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Increased BCA-1 Expression and Attenuated Apoptosis in Epithelial Cells in Helicobacter pylori-associated Low Grade Gastric MALT Lymphogenesis
… to MALT lymphoma. MATERIALS AND METHOD: Using tissue blocks from 20 patients with H. pylori-positive gastritis, 39 patients with H. pylori-positive low grade MALT lymphoma, and 18 patients with H. pylori-negative gastritis, in situ hybridization for BCA-1 and its receptor, CXCR5, and TUNEL …
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Investigating the relationship between miRNA expression and epithelial mesenchymal transition in colorectal cancer
… cases) Formalin Fixed Paraffin Embedded (FFPE) tissue blocks and their corresponding H&E slides were collected from the archives of the Division of Anatomical Pathology at the University of Cape Town. Subsequently, the FFPE tissue blocks were sectioned at 3μm and IHC analysis of 4 EMT markers …
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Immunohistochemical Evaluation and Gene Expression Profiling of Senile Lentigo
… study, formalin-fixed and paraffin-embedded tissue blocks obtained from 20 Korean patients (1 man, 19 women) were used. Immunohistochemical staining of tyrosinase, NKI/beteb, MITF (microphthalmia-associated transcription factor), Ki67, SFRP2 (secreted frizzled-related protein 2), Wnt-5a, p16 …
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Exploration of parameters affecting jet injection using a high-speed X-ray imaging system
… distribution and deformation of the porcine tissue block used in the jet injection experiment. The parameters for the high-speed X-ray imaging system, namely the voltage and the current of the X-ray source and the exposure time of the high-speed camera, were optimized for tissue blocks with …
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ROR1 and Rab27b – novel biomarkers for chemoresistance in ovarian cancer
… through PCR-based pilot study on ovarian cancer tissue blocks. The aim of this project was to investigate ROR1 and Rab27b as biomarkers for chemoresistance in ovarian cancer. Ovarian cancer cell line panel was assembled comprising of; HEY, SKOV-3, OVCAR-3 and OAW42. The IC50 doses of four drugs; …
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Biomechanics of Cardiac Remodelling in Heart Failure
… stain was applied to samples of myocardial tissue blocks, which were then imaged using extended-volume confocal microscopy. Previous studies have shown that SHRs have greater deposition of perimysial collagen between sheetlets, and thickening of endomysial collagen compared with control …
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Immunohistochemical Characterization of Breast Cancer at the University Teaching Hospital, Lusaka, Zambia
… select the 44 formalin fixed-paraffin embedded tissue blocks of breast tissue diagnosed with breast cancer on histology for the year 2012. The specimens were from patients aged between 21 and 70 years old. Tissue sectioning, H & E staining, cover slipping and microscopic examinations was done …
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Immunohistochemical Characterization of Breast Cancer at the University Teaching Hospital, Lusaka, Zambia
… select the 44 formalin fixed-paraffin embedded tissue blocks of breast tissue diagnosed with breast cancer on histology for the year 2012. The specimens were from patients aged between 21 and 70 years old. Tissue sectioning, H & E staining, cover slipping and microscopic examinations was done …
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Studies of proteins in the posterior lobe of the pituitary gland
… used to demonstrate neurophysin-like antigen in tissue slices. Initially tissue was frozen unfixed and sectioned in a cryostat prior to fixation in 95% (v/v) aqueous ethanol. subsequently an improved method was developed in which tissues were fixed in saline-formalin and embedded in paraffin and …
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The relationship between S. aureus biofilm properties, inflammation, and disease severity in chronic rhinosinusitis
… inflammation of the sinus mucosa associated with tissue remodelling, dysfunction of the sinuses' natural defence mechanisms, and induction of different inflammatory clusters. Staphylococcus aureus mucosal biofilms are associated with severe recalcitrant CRS. However, S. aureus colonisation of the …
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The relationship between BRAF genetic alterations and the risk of tumour recurrence in Pilocytic Astrocytomas in children diagnosed at Red Cross Children's Hospital over a 33-year period
… the diagnosis was confirmed on histology, and tissue blocks were retrieved. The presence of BRAF genetic aberrations were examined using immunohistochemistry, FISH and PCR. Results: Sixty-nine paediatric patients with PAs were identified over the study period with a median age of 6 years. This …
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Diagnostic Neuropathology of Brain Tumours using Biophotonics and Spectrometry
… at two approaches, elemental profiling of both tissue and serum using inductively coupled plasma-mass spectrometry. Trace elements increase or deficiency has been linked to cancer development and progression. The final study looked at the diagnostic application of Raman spectroscopy to …
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Applications of Physiologically Based Pharmacokinetic Models in Veterinary Medicine.
… use of sulfamethazine in swine. This model had tissue blocks for all edible tissues. Correlation coefficients for each tissue ranged from 0.86 to 0.99. The model accurately predicted withdrawal intervals after intravenous extralabel drug use. This model was expanded to include population …
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