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Showing 1 to 8 of 8 for “"TIRFM"”.
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Development of a TIRF Microscope for Single Molecule Binding Studies
… internal reflection fluorescence microscope (TIRFM) is necessary to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal reflection (TIR) to …
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Entwicklung und Aufbau einer modularen Konfokal-Multiphotonen-Laserscanning-Messapparatur (CMLTT) für Second Harmonic Generation-, Total Internal Reflectance- und Laser Tweezers-Anwendungen an myofibrillären Präparaten
… Modul für innere Totalreflektionsmikroskopie (TIRFM), eine zusätzliche stationäre Multiphotoneneinkopplung sowie Positionsdetektoren enthält. Dabei wurde diese CMLTT-Gesamtapparatur so konzipiert, dass die einzelnen Module weitgehend parallel einsetzbar sind. Der Laserstrahl des Laser tweezers- …
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Photonische Rasterkraftmikroskopie an fluoreszierenden Nanokristallen und molekularen Photoschaltern
… mit evaneszenter Probenbeleuchtung (TIRFM) vorgestellt. Dieser Aufbau ermöglicht die externe Kontrolle der Fluoreszenzemission einzelner Halbleiter-Nanokristalle: Die Variation des Abstandes zwischen Fluorophor und einem Gold modifizierten Kraftsensor führt zu einer messbaren …
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The photophysics properties of lanthanide luminescent probes
… internal reflection fluorescence microscopy (TIRFM). The results indicate feasibility of using lanthanide luminescent probes for time-gated luminescence microscopy.
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A large scale analysis of chemoattractant induced cytoskeletal dynamics in Dictyostelium discoideum by means of proteomic and imaging approach
… distinct incorporation dynamics detected by TIRFM imaging. The first group includes DocA, DocB and GacR proteins, which show very rapid and transient translocation to the cortex and are most likely involved in the earliest events following cAMP stimulation. The second group, which is composed …
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Engineering fluorescent imaging platforms to characterise molecular interactions with HIV-1 capsid
… first approach focused on the development of a TIRFM biosensor using CA tubes immobilised on a glass surface as the biorecognition substrate for sensitive single-molecule binding studies. The biosensor was assessed in its ability to measure binding affinity, stoichiometry and kinetics using …
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The Initial Events of T-Cell Activation in Realistic Model Systems
T cells are part of the cellular component of the adaptive immune system and specifically recognise foreign antigens in the body. They use their membrane-bound T-cell receptor (TCR) to bind small peptide antigens presented within the major histocompatibility complex (pMHC) on the surface of antigen …