Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"THP1"”.
-
Identification of exosomal proteins in primary human bronchial tracheal epithelial cell HBTE and the H358, THP1 and MCF7 cell lines
… cancer cell lines (lung cancer H358, leukaemia THP1 and breast cancer MCF7) and a primary lung cell HBTE were isolated and their protein contents were analysed to establish whether cancer specific proteins are present. Methods and Materials: Exosomes were isolated and characterized by scanning …
-
Modulation of Brain Inflammation Induced by Interaction between Astrocytes, Neutrophils and Monocytes
… between astrocytes and monocytes 20 1. Effect of THP1 cells on morphology of astrocytes 20 2. Effect of THP1 cells on inflammatory responses of astrocytes 24 3. Migration assay 26 4. Dose-dependent effect of TCM 29 5. Astrocytes regulate inflammatory responses in THP1 cells 29 IV. DISCUSSION 32 V. …
-
Expression und Regulation 1,25(OH) 2 -Vitamin D 3-responsiver Gene in monozytären Zellen
… peripheren Blutmonozyten (PBM) und monozytären THP1-Zellen untersucht. Durch Supplementation der Zellen mit dem Substrat 25(OH) Vitamin D3 konnte die Produktion an aktivem 1,25(OH)2 Vitamin D3-Hormon in PBM signifikant gesteigert werden. In PBM konnte im Gegensatz zum systemischen …
-
The synthesis, characterization, and use of a protein-cysteine proteinase inhibitor complex for the study of endosome/lysosome fusion
… Their presence in human cell monocytic lines THP1 and U937 was detected by the use of the membrane-permeable, irreversible, active-site directed inhibitor Fmoc-(¹²⁵I)Tyr- Ala-CHN₂ followed by immunoprecipitation of the enzymes, SDSPAGE, and autoradiography. All three enzymes were detected in …
-
Multi-Omics Investigation to on the Effect of Replication Stress on Leukemia Cells
… many chromatin modifier genes that suppressed THP1 differentiation did not also suppress differentiation in K562s. Thus, the mechanism of how replication stress leads to differentiation is still unknown.
-
Role of microRNA-155 in dendritic cells and macrophages: MiR-155 directly targets PU.1 and IL13Rα1
… transgene under the control of a Tet-On system (THP1-155 cells), both PU.1 and IL13Rα1 were shown to be down regulated following miR-155 induction in these cells. Moreover, THP1-155 cells showed that DC-SIGN transcription is regulated by miR-155 levels through PU.1 targeting, and that IL-13 …
-
Regulation of Cell Invasion by the Podosome/Invadopod Protein Tks5
… and wound healing. Using model THP1 macrophages, my studies have shown that Tks5 can directly control podosome-associated matrix degradation and invasion through collective changes in adhesion, chemotaxis, and the expression/proteolytic activity of MMP9. I have further shown that …
-
Zinc Finger Protein 36L1 (ZFP36L1): Gene Expression, Regulation and Interactions with Immune Receptors in Human Tumour Cells.
… of ZFP36L1 in several tumour cell lines such as THP1, Jurkat, B16, MCF7, and MC38. To study the function of ZFP36L1 a CRISPR-Cas9 system was used to generate ZFP36L1 knockout MC38 cell line. Our results show that ZFP36L1 limits production of TNF α and VEGF A. Our preliminary data also show …
-
Atypical Mechanisms of Synthesis, Activation and Release of Interleukin-1β from Vascular Smooth Muscle Cells
… activity than equal amounts of mature IL-1β from Thp1 cells, however the existence of a binding partner or inhibitor of IL-1β is unlikely the cause of this phenomenon. In conclusion, the work presented in this thesis suggests that VSMCs have a novel mechanism that specifically prevents the release …
-
The effects of quercetin on iron metabolism
… contrast to findings in Caco-2 cells, in Thp1 macrophages quercetin caused a significant dose-dependent increase in FPN expression. Furthermore, quercetin induced both FPN1A and 1B promoter activities. This strongly implies that quercetin acts at the level of the FPN promoter to increase …
-
Manipulating PLGA nanoparticle cellular uptake and trafficking
… and two macrophage cell lines (RAW264.7 and THP1). In addition, the dependance of NP uptake upon the clathrin-dependent endocytosis pathway was investigated. In order to translate our observations in regard to NP size-dependent uptake, ciprofloxacin-loaded and ceftazidime-loaded PLGA NPs were …
-
The novel phosphorylation site PLCγ2 Y1217 facilitates the TREM2 amyloid-β sensing pathway in microglia
… that intracellular Ca<sup>2+</sup> release in THP1 cells in response to Aβ oligomers was impaired when TREM2 was silenced by siRNA, indicating the TREM2 pathway is reliant on calcium flux to facilitate its signalling response to Aβ oligomers. Next, I evaluated microglial density and plaque …
-
Development of versatile drug delivery strategies using PLGA nanoparticles
… camptothecin demonstrated significant targeted THP1 leukemic cells toxicity. Therefore sialic acid conjugated NP may have therapeutic usefulness in the targeting of myeloid cells in a range of disease conditions such as acute lung injury and leukaemia.<br/>In conclusion, the results presented …
-
Host cell-pathogen interactions between erythrocytes and P. falciparum: apoptotic mimicry?
Understanding the phagocytic process of malaria-infected erythrocytes and the subsequent cascade of host response are important in developing new strategies for treating malaria. In this study, we focused on phosphatidylserine (PS) and its possible role in malaria pathogenesis through phagocytosis. …
-
NLRP2 expressziójának vizsgálata különböző immortalizált sejtvonalakon
Célunk ezen in vitro kísérletsorozat során az volt, hogy megvizsgáljuk a GM-CSF és a dexametazon NLRP2 receptor expressziójára gyakorolt hatását különböző immortalizált humán sejtvonalakon. A kísérletek során THP-1, K562, Caco2, A549, NB4, U937, Hela és MCF-7 sejtvonalakat használtunk, melyeket 6, …