Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 131 for “"THP-1"”.
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Inflammasome activation in THP-1 target cells triggered by pathogenic Naegleria fowleri
… to N. fowleri infection, human macrophage cells (THP-1 cells) were co-cultured with N. fowleri trophozoites under the non-contact system, and consequently IL-1β production was estimated. By western blotting and ELISA analysis, the caspase-1 activation and IL-1β production from THP-1 cells and the …
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The effects of metolachlor exposure on the THP-1 alveolar monocyte and macrophage function
1 PDF file (vii, 88 pages)
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Investigating the anti-inflammatory effects of plant derived compounds in tuberculosis using the THP-1 macrophage model
Background Tuberculosis (TB) is a highly communicable disease that is caused by the bacillus, Mycobacterium tuberculosis. TB is responsible for devastating morbidity and mortality worldwide, especially in the developing world. TB can be effectively treated with the currently available drugs; …
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Role of Vitamin-D3 and Retinoic Acid In A Human Thp-1 Macrophage Model of Mycobacterium Tuberculosis Infection
… these vitamins (RAVD) enhanced the receptors on THP-1 macrophage (Mannose receptor and DC-SIGN) that increased mycobacterial uptake but inhibited the<br />subsequent intracellular growth of Mtb by inducing reactive oxygen species (ROS) and autophagy. RAVD also enhanced antigen presenting and …
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Impact des microvésicules de plaquettes sur l'expression de produits inflammatoires des monocytes et macrophages THP-1
… de flux que le modèle cellulaire monocytaire THP-1 et les macrophages qui en sont dérivés captent les PMVs. Leur capacité d’internaliser ces PMVs fut démontrée par microscopie confocale. Pour évaluer le transfert potentiel de contenu, les transcrits et les protéines des gènes prostaglandine …
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Identification of a novel method for differentiating human monocytic cell line into macrophages
… may stimulate in vivo using a monocytic cell (THP-1) and a human liver cell (HUH7). The basis for choosing these two cells stemmed from research by Matak et al confirming their cellular communication. Aim: The aim of the research was to compare two different methods of THP-1 monocyte …
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Development and Characterisation of an Immunocompetent Three-Dimensional Oral Mucosal Infection Model
… (3DOMMs) were further developed to incorporate THP-1 pro-monocyte cells into the model matrix producing a novel immunocompetent oral mucosal model (IC3DOMM). Models were characterised using a variety of histological, biochemical, and molecular techniques to detail the model microenvironment and …
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Regulation of the Gene Encoding Thrombin-Activable Fibrinolysis Inhibitor in Non-Hepatic Cells
… lines MEG-01 and Dami, the monocytic cell line THP-1, and peripheral blood mononuclear cells. TAFI protein was detected in differentiated Dami and THP-1 cells. We next describe the effect of external stimuli such as phorbol myristate acetate (PMA) on CPB2 expression in Dami and THP-1 cells. We …
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A study of the metabolic immune responses produced by some natural products
… production and the metabolic profiles of THP-1 monocyte-derived macrophage cells following stimulation with LPS, alone or in combination with different natural products.;Some of these natural products, such as melittin and eicosanoids, were considered to act as vaccine adjuvants and to …
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NLRP2 expressziójának vizsgálata különböző immortalizált sejtvonalakon
… humán sejtvonalakon. A kísérletek során THP-1, K562, Caco2, A549, NB4, U937, Hela és MCF-7 sejtvonalakat használtunk, melyeket 6, valamint 24 órás GM-CSF és dexametazon kezeléseknek vetettük alá. A kezelt sejtekből RNS-t izoláltunk, majd RT-qPCR segítségével meghatároztuk az NLRP2 relatív …
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Novel cytokines in innate immunity: IL-27 and IL-30 enhance responsiveness to gram-negative bacterial components in human myeloid cells
… TLR4 and P2X7 expression. Comparing human THP-1 monocytes and PMA-differentiated THP-1 macrophages, CD14 expression plays a role in LPS and IL-27 responsiveness, whereby IL-27-mediated inhibition of LPS tolerance is greater in CD14low THP-1 monocytes compared to CD14high PMA-differentiated …
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MyD88-Mediated Signaling in Protective Immunity against an Attenuated West Nile Virus Infection
… to young DCs. I also used two human cell lines-THP-1 and THP-1 macrophages to study the immune response following NS4B-P38G infection. NS4B-P38G mutant produced more viral RNA than parental NY99 in both cell types and boosted higher innate cytokine responses with no detectable infective virus. …
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Spontaneous Raman spectroscopy: Exploring applicability in drug discovery and the medical sciences
… of whole cell concentration of compounds inside THP-1 and Madin Darby Canine Kidney (MDCK) cells. For true quantification the Cell Silent Region (CSR) is used to measure without interference from cellular background signal. The model compound is erlotinib, an anti-cancer drug with an alkyne group …
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Characterization of interleukin-4 induced gene-1 (IL-4I1) as a host immunoregulator to cutaneous leishmaniasis in cell based models
… and IL-4i1-/- mice and silencing of IL-4i1 in THP-1-derived macrophages. THP-1 monocytes were differentiated into macrophages via phorbol 12-myristate 13-acetate (PMA). Following macrophage verification, THP-1-derived macrophages were silenced with IL-4i1 siRNA constructs using the Silencer …
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N-terminomics/proteomics investigation of Calpain-2 substrates
… experiment in calpain-2 knockout human THP-1 monocytic cell lines to identify new substrates. We analyzed the proteomics and Nterminomics data using bioinformatics and pathway enrichment tools (STRING-db (https://string-db.org) and Metascape (https://metascape.org/gp/index.html)). We …
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Regulation of TNF-alpha gene expression by TNFAIP1 as an activator or suppressor in response to lipopolysaccharide
… A culture of mouse RAW cells and human THP-1 cells in RPMI media supplemented with 10% FBS at 37°C in 5% CO2, Polymerase Chain reaction (PCR) using specifically designed primers and DNA cloning of TNFAIP1, transfection of the cloned TNFAIP1 cDNA into macrophages, Western blot analysis, …
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Stimulation humaner V-Gamma-9-V-Delta-2-T-Lymphozyten : Untersuchungen zur Wirkung Stickstoff-haltiger Bisphosphonate und zu bakteriellen Phosphoantigenen
… mit Zoledronat wurde die monozytäre Zelllinie THP-1 stimulierend für Vg9Vd2-T-Zellen. Auch weitere Zelllinien und Lymphozyten des peripheren Bluts (PBL) konnten nach Vorinkubation mit Zoledronat Vg9Vd2-T-Zellen aktivieren. Dabei genügten bei den PBL deutlich geringere Zoledronat-Konzentrationen …
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