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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"TFO"”.
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Specificity of triple helix formation
Triplex-forming oligonucleotides (TFOs) have been the subject of extensive<br/>research in recent years. They have potential applications in many areas; such as<br/>gene-based therapies, site-directed mutation and as biochemical tools. However,<br/>triplex technology has been hampered by several …
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Therapeutic Gene Delivery to Human Pancreatic Islets for Treatment of Diabetes and the Effect of TFO on Liver Fibrosis Induced by Bile Duct Ligation
… used a triplex forming oligonucleotide (TFO) against the type α1 (I) collagen and evaluated, whether it can attenuate liver fibrosis induced by common bile duct ligation (CBDL). There was a significant decrease in hydroxyproline levels in TFO‑treated groups compared to non‑treated CBDL …
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DNA Triplexes in chemistry biology and medicine
… analogue into triplex forming oligonucleotides (TFOs). It was compared with other cytosine analogues, 5-methyl-(2’-deoxy-?-D-ribofuranosyl)cytosine (MeC), 2’-Omethyl MeP (MePOMe), and 2’-O-aminoethyl MeC (MeCAE). Triplex stability studies indicate that over the pH range 6.2-8.0, the general trend …
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The Development of New Routes to Half-Sandwich Metallatricarbadecaborane Complexes, Triflate-Functionalized Polyboranes and Carboranes, and Ammonia Borane Hydrogen Release
… to synthesize and explore the reactivity of 5-TfO-B10H13, and (3) to investigate metal-catalyzed hydrogen release from ammonia borane in ionic liquids. The major achievements are outlined below. Chapter 1 describes the synthesis and structural characterization of the first series of group 9 …
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Synthesis of triplex-forming oligonucleotide conjugates of the anticancer drug temodal
… to triplex-forming oligonucleotides (TFOs) is a potential way of targeting these alkylating agents to specific gene sequences to maximise site-selectivity. In this work, polypyrimidine TFO conjugates of both drugs were synthesised and targeted to duplex DNA in an attempt to effect …
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Development and Evaluation of Nano‑scale Systems for Targeted Delivery to Treat Liver Fibrosis
… synthesis, one triplex forming oligonucleotides (TFO), was systemically administrated to prevent type I collagen mRNA transcription. To enhance circulation time and targeted delivery efficiency, TFO was conjugated to M6P‑HPMA and showed efficient targeted delivery to HSCs. Two week short term in …
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Nucleotide Excision Repair, Crosslink Repair and Transcriptional Function of Xpa In Human Cells
… repair of a triplex forming oligonucleotide (TFO)-directed psoralen ICL. A TFO-directed psoralen DNA ICL was constructed in closed-circular DNA. In NER proficient human cell extracts, incisions were detected on both strands of the damaged DNA 3’ to the psoralen ICL. Incision sites on the TFO …
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Room Temperature Ionic Liquid (Rtil) Membranes For Bio-Methane Dehumidification
… membrane based on the trifluoromethanesulfonate [TfO] anion had a water permeance of 2000 GPUs with a H2O/CH 4 selectivity of 16000. The bulk of the RTILs exhibited similar water permeances ranging from ∼700-900 GPU. The methane permeances ranged from 0.15 GPU–0.5 GPU with [emim][TfO] having the …
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Reverse-Selective Behavior Of The Room Temperature Ionic Liquid Based Membranes
… support and [emim][Tf2N], [emim][BF4], [emim][TfO], [emim][DCA], [emim][SCN], and [bmim][NO3] as immobilized room temperature ionic liquid (RTIL). The results shothat permeance of carbon dioxide was the highest in [emim][Tf2N], while it was the lowest in [bmim][NO3]. Moreover, permeance of the …
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Gene Regulation Through Artificially Induced DNA Conformational Changes
… we used tethered-triple helix oligonucleotides (TFO) to bend DNA. We show that the expression of a luciferase gene is regulated by the presence of an induced DNA bend. Bends occurring in the same orientation as RNA polymerase binding result in a 93% increase in expression. In contrast, bends …
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Metagenomic characterization of Candidatatus Defluviicoccus tetraformis TFO71, a tetrad-forming organism, predominant in an anaerobic-aerobic membrane bioreactor with deteriorated biological phosphorus removal
… tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant DTFO, “Candidatus Defluviicoccus tetraformis TFO71,” was successfully constructed and characterized. Examining the genome confirmed …
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Novel nucleotide analogues for forming stable DNA triple helices
… by binding of a triplex-forming oligonucleotide (TFO) to a DNA duplex, via specific recognition of the individual base pairs in the target sequence.<br/>Mixed-sequence recognition of duplex DNA by TFOs is therefore an essential requirement for successful targeting. However, achieving strong, yet …
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Influence of Electrostatic and Intermolecular Interactions on the Solution Behavior and Electrospinning of Functional Nanofibers
… Cl-, NO3-, (CN)2N-, BF4-, PF6-, triflate (TfO-), and bis(trifluoromethanesulfonyl)imide (Tf2N-). The counteranion selection controlled the thermal transitions and degradation; the larger and more charge-delocalized anions typically resulted in lower Tg and higher decomposition temperature. …
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Synthesis and Characterization of Zwitterion-Containing Acrylic (Block) Copolymers for Emerging Electroactive and Biomedical Applications
… capacity of the triblock copolymers for EMIm TfO, an ionic liquid. DSC and DMA results revealed the impact of the ionic liquid on the thermal and thermomechanical properties of the triblock copolymers, respectively. Composite membranes of DMAEA-derived triblock copolymers and EMIm TfO …
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Optimization of Gold Nanoparticles for Radiotherapy
… as the DNA triplex forming oligonucleotides (TFOs) attached to the AuNPs, is still responsible for a radiosensitisation effect, although smaller than with sugar:PEGamine AuNPs. In the future, the uptake of the oligonucleotides AuNPs may possibly be improved by varying their size (from 3.5 to 2 …
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Tailoring Structure and Function of Imidazole-Containing Block Copolymers for Emerging Applications from Gene Delivery to Electromechanical Devices
… (BF4), trifluoromethanesulfonate (TfO), and bis(trifluoromethanesulfonyl)imide (Tf2N). Thermogravimetric analysis revealed that thermal stability increased with decreased alkyl substituent length and larger counteranion size, and differential scanning calorimetry determined that …