Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"TFIID"”.
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TATA binding protein and associated factors in mammalian transcription : characterization of the B-TFIID activity
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1994.
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The Sumoylation of General Transcription Factors Regulates Transcription in Saccharomyces cerevisiae
… have determined that transcription factor IID (TFIID) and transcription factor IIF (TFIIF) are targets of sumoylation. The functional impacts of GTF sumoylation were assayed using strains with elevated or reduced TFIID or TFIIF sumoylation. Using chromatin immunoprecipitation (ChIP), we found …
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A Molecular Characterization of Human General Transcription Factor IID
… encoding a protein that may substitute native TFIID fractions in DNA binding and in vitro basal transcription assays. Sequence comparisons identify important structural motifs in the protein. Further functional analyses lead to the realization that this protein is the TATA box binding subunit …
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The Role of Phosphorylation in Modulating PC4 Function as a General Cofactor
… transcription, regardless of whether TBP or TFIID is used as the TATA-binding factor. Interestingly, repression of basal transcription by the positive cofactor PC4 is alleviated by increasing amounts of TFIID, TFIIH and a preassembled RNA polymerase II holoenzyme complex but not by the other …
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Transcriptional Regulation Mediated by Human Papillomavirus E2 Protein
… E2 does not simply work by displacing TBP or TFIID from binding to the adjacent TATA box. Instead, E2 appears to function as an active repressor that directly inhibits HPV transcription at steps after TATA recognition by TBP or TFIID. To investigate whether E2 proteins encoded by high-risk …
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Identification des éléments CIS d'ARN et développement d'un gène rapporteur pour caractériser les facteurs d'épissage qui contrôlent l'expression du facteur de transcription pro-apoptotique TAF6[delta]
… dont le facteur de reconnaissance du promoteur, TFIID. TFIID est un complexe multi-protéique composé de la protéine de liaison de la boîte TATA (TBP) et de 14 facteurs associés à TBP (TAFs) tel que TAF6 qui nous intéresse. TAF6 est exprimé sous 5 isoformes d'épissage alternatif (?, ?, ?, ð, ? ). …
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
… mechanism in eukaryotes: activators target TFIID (TBP)-TFIIB promoter complex formation in the preinitiation complex assembly process by inducing (or stabilizing) qualitative or quantitative alterations within TFIID (TBP)-TFIIB-promoter complexes that consequently enhance recruitment of …
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Funktionale Charakterisierung der Kinase-Aktivität des Transkriptionskoaktivators TAFII250 in Drosophila melanogaster
… des generellen Transkriptionsfaktors TFIID. Indem TAFII250 sowohl mit TBP, als auch mit nahezu allen TAFII-Untereinheiten interagiert, nimmt es eine zentrale Stellung inner-halb des TFIID-Komplexes ein. Zudem ist TAFII250 an der Erkennung der Initiator-Promotorsequenz beteiligt, …
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Preinitiation Complex Assembly and General Transcription Factor IIA
… namely the formation of complexes including TFIID, TFIIA, and TFIIB. To further the study of this complex, attention is then turned to TFIIA. A eDNA is isolated encoding the small subunit of TFIIA from Drosophila melanogaster. The protein encoded by this eDNA, dTFIIA-S, is highly homologous …
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Mechanistic Insights into Direct Interactions that Mediate the Activity of DOT1L Complex in MLL-Rearranged Leukemia
… assays reveal a direct interaction between TFIID and the DOT1L complex, with H2Bub and DOT1L protein enhancing the recruitment of TFIID to chromatin. These direct interactions implicate the DOT1L complex in transcriptional initiation. In summary, this study advances our understanding of the …
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Genome-wide analysis of transcription factors and chromatin in S. cerevisiae
… SrblO, Swi2), the general transcription factor TFIID (Taf145, Taf17), and the SAGA histone-modifying complex (Gcn5, Taf17) were found not to be required for the transcription of all yeast genes but for distinct subsets of genes, revealing a surprising level of gene-specific regulation by the …
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Lysine acetyltransferase Gcn5-B regulates the expression of crucial genes in Toxoplasma and its function is regulated through lysine acetylation
… complex” that includes the co-activator ADA2-A, TFIID subunits, LEO1 polymerase-associated factor (Paf1) subunit, and RRM proteins. The dominant-negative phenotype of ddHAGCN5b(E703G) parasites, considered with the proteomics and ChIP-chip data, indicate that GCN5b plays a central role in …
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Molekulare Mechanismen der transkriptionellen Aktivierung durch die Transkriptionsfaktoren c-Jun und v-Jun
… war. Die vorliegenden Daten zeigen, dass die TFIID- Untereinheit hTAFII55 die c-Jun vermittelte transkriptionelle Aktivierung st
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Genome-wide expression and location profiling in Saccharomyces cerevisiae : experimental and graphical analysis
… II holoenzyme, the general transcription factor TFIID, and the SAGA chromatin modification complex were found to have roles in expression of distinct sets of genes. The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a …
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Physiological Bases and a Novel Genetic Determinant of Water-Use Efficiency (WUE)
… associated factor (TAF) constituents of the TFIID complex and a loss-of-function mutant <em>(taf10)</em> has a 16% increase in WUE without a decrease in biomass production, relative to wild type (WT) plants. The improved WUE is due to a decrease in transpirational water loss that is …
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General Transcription Factors Play Dual Roles In Initiation And Termination
… was intermediate between that of TFIIH and TFIID. Initiation factors which remain bound on a promoter scaffold in vitro, were not found in a holo-TFIIB complex with termination factors. The holo-TFIIB complex was observed only in the looping competent strains, but not in the looping …
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Transcriptional Regulatory Mechanisms of Ribosomal Protein Genes
… impaired association of NuA4 interferes with TFIID recruitment, but recruits TAF-independent form of TBP to the core promoter. This recruitment of TBP is dependent on SAGA (Spt-Ada-Gcn5-acetyltransferase). Like ribosomal protein genes, antisense transcription is also enhanced by TAFs. However, …
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Identifying natural modifiers of meiotic crossover frequency in Arabidopsis thaliana
… in Bur-0 (taf4b-1). TAF4b is a subunit of the TFIID complex, a multi-protein general transcription factor complex comprising TBP and numerous TAFs that forms a component of the pre-initiation complex that recruits RNA polymerase II to promoters. Transformation-based complementation experiments …
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Transcriptional regulation of the Human Zfm1/Sf1 Gene
… Komponenten des basalen Transkriptionsfaktors TFIID assoziiert ist, namentlich hTAFII68 und hTAFII80. Zusammengefasst zeigen diese Ergebnisse, das Zfm1 seine Rolle als transkriptioneller Repressor auf der Ebene der basalen Transkriptions-maschinerie ausübt und dass seine EGR-1-vermittelte …
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Cloning, sequencing and functional analysis of the chicken tyrosine gene promoter
… and therefore are unlikely to function as TFIID-binding sites. Transcription initiation appears to occur at heterogeneous start sites, and given the absence of a functional TATA box, may be mediated via the conserved initiator region and SP1-binding site. To test the ability of the 5' …