Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"TFIIB"”.
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Expression and initial characterisation of the Plasmodium falciparum general transcription factors TFIIB and TLP
… anti-malarials. The general transcription factor TFIIB and the TBP-like protein (TLP) are key proteins involved in the recognition of the core promoter, and the initiation of RNAPII transcription initiation complex assembly. TFIIB stabilises DNA binding of the primary promoter recognition factor, …
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General Transcription Factors Play Dual Roles In Initiation And Termination
… is unknown. The general transcription factors TFIIB and TFIIH have been found to interact genetically with Ssu72, a component of CPF 3' end processing complex. Accordingly, we found that TFIIB and TFIIH localize to the distal ends of genes in a transcription dependent manner. TFIIB localization …
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
… in eukaryotes: activators target TFIID (TBP)-TFIIB promoter complex formation in the preinitiation complex assembly process by inducing (or stabilizing) qualitative or quantitative alterations within TFIID (TBP)-TFIIB-promoter complexes that consequently enhance recruitment of downstream …
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Transcription regulation in Plasmodium falciparum : functional characterisation of general transcription factor IIB
… protein (TBP) and transcription factor IIB (TFIIB) are the key protein factors involved in the core promoter recognition and RNAPII preinitiation complex (PIC) assembly. TBP nucleates PIC assembly by binding the TATA box, thereafter the TBP-TATA complex is further stabilised by TFIIB. In …
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Prevalence And Physiological Significance Of Gene Looping In Saccharomyces Cerevisiae
… of gene looping was analyzed by genomewide TFIIB ChIP-Seq. The simultaneous presence of TFIIB on both the promoter and terminator of a gene was taken as a measure of gene looping. Our results suggest that gene looping is not restricted to a few transcriptionally active genes in yeast, but is …
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Functional divergence of the RNA polymerase II transcription machinery in Plasmodium falciparum
… orthologue of general transcription factor IIB (TFIIB). Through the biochemical characterisation of protein-DNA interactions, protein-protein interactions and the biomolecular condensation properties of these transcription factors, this study aimed to provide insight into the molecular mechanisms …
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Preinitiation Complex Assembly and General Transcription Factor IIA
… a partial preinitiation complex containing TFIIB is implicated as a target of GaI4-IE. These experiments, as well as others cited, suggest that activators affect early stages of PIC assembly, namely the formation of complexes including TFIID, TFIIA, and TFIIB. To further the study of this …
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Shield and Shuttle: Importins as Protective Chaperones and the Nuclear Transport Mechanism of H2A-H2B
… H2A-H2B and transcription factors TBP and TFIIB for yeast Importin Kap114. It has been shown that these cargos need both RanGTP and DNA to be fully released from the importin, suggesting these cargos are chaperoned for targeted delivery. Here, I will further discuss how I played a part in …
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Impact of genetics and phosphorous nutrition on bone metabolism
… There was also a trend of increased IL6, TFIIB, and S0X9 expression with P deficiency in HB, but not LB, pigs. A trend of increased expression of OXTR was seen in P deficient animals in both genetic backgrounds. Expression of RANKL, BGLAP, OPG, ARAF1, and IGFBP5 was not affected by genetic …
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GENOME-WIDE PROTEIN MOLECULAR ARCHITECTURE OF HUMAN PROMOTERS
… (P300), the transcription machinery (TBP, TFIIB, Pol II), and nucleosomes at promoters. We propose a refined model of human promoters characterized by bidirectional transcription, where two distinct PICs initiate on opposing strands while sharing common upstream regulatory regions. Our …
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Charakterisierung des negativen Cofaktors 2 der RNA-Polymerase II in vivo und in vitro
… der generellen Transkriptionsfaktoren TFIIA und TFIIB und führt dadurch zur Unterbrechung der Bildung des Initiationskomplexes. NC2 besteht aus zwei Untereinheiten, NC2a und NC2b, die starke Homologien zu den Histonen H2A bzw. H2B aufweisen. Alle Erkenntnisse zu Beginn dieser Arbeit basierten auf …