Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"TET1"”.
-
Tet1: A Unique Dna Demethylase For Maintenance of Dna Methylation Pattern
… The ten-eleven translocation family proteins (TET1/2/3) were recently identified as Fe(II)/2-oxoglutarate (2OG)-dependent 5mC dioxygenases, which consecutively convert 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine and 5-carboxylcytosine both in vitro and in mammalian cells. Based on …
-
The role of Arid1a and Tet1 in ductal cell-driven liver regeneration
Title: The role of Arid1a and Tet1 in ductal cell-driven regeneration Author: Mikel Alexander Mckie The liver has several robust and potent mechanisms of repair after damage despite slow homeostatic turnover. In cases of extreme toxic damage, where the hepatocyte compartment is severely compromised …
-
TET1 AND TRANSCRIPTION FACTOR REGULATION OF LINEAGE SPECIFICATION DURING MOUSE GASTRULATION AND EARLY ORGANOGENESIS
… Translocation methylcytosine dioxygenase 1 (TET1), an epigenome modifier, which regulate cell fate during gastrulation and early organogenesis in mouse. Following unbiased epigenetic and proteomic screens for TF regulators and chromatin-bound TET1 proximal (“proxisome”) proteins, I use …
-
The Role of TET Proteins in the Epigenetic Regulation of Neural Gene Expression and Behavior
… neural functions, we generated new lines of Tet1 and Tet3 mutant mice using a gene targeting approach. We designed both mutations to cause a frameshift by deleting the largest coding exon of Tet1 (Tet1Δe4) and the catalytic domain of Tet3 (Tet3Δe7-9). As Tet1 is also highly expressed in …
-
ELUCIDATION OF MECHANISMS GENERATING 5-HYDROXYMETHYLCYTOSINE (5hmC) IN MAMMALIAN MITOCHONDRIA
… the Tet family of methylcytosine dioxygenases(Tet1, Tet2 and Tet3) was found to catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), an intermediate in the pathway of DNA demethylation. Tet enzymes catalyze this hydroxylation in a 2-oxoglutarate and Fe2+ dependent …
-
The Epigenetic Role of EGR1 during Postnatal Mammalian Brain Development
… that EGR1 recruits a DNA demethylase TET1 to remove the methylation marks at EGR1 binding sites and activate downstream genes. Also, we found that the frontal cortices from the knockout mice lacking Egr1 or Tet1 share strikingly similar profiles in both gene expression and DNA …
-
Maintaining Proper Levels of DNA Methylation Marks Through the TET Family is Critical for Normal Embryo Development in Pigs
… (5hmC). The three members of TET protein family, TET1, TET2, and TET3, are highly expressed in preimplantation embryos in a stage-specific manner. Previous studies demonstrated that TET proteins are involved in diverse biological processes such as gene regulation, pluripotency maintenance, and …
-
The Role of 5-Hydroxymethylcytosine in Genome Instability
… 5hmC, produced via overexpression of the TET1 catalytic domain, is unlikely to threaten genome stability via deamination in the nucleotide pool based on the observation that loss of the TDG DNA glycosylase, which excises 5fC and 5caC, rescues the sensitivity of Polb-/- cells to TET1-CD …
-
Characterization of 5-methylcytosine dioxygenase Tet2 and rescue of mutant Tet2 activity by using turbo co-substrate
… by ten-eleven translocation dioxygenases (TET1-3), followed by thymine-DNA glycosylase-dependent base excision repair. Inactivation of the TET2 gene due to genetic mutations or other epigenetic mechanisms is associated with poor prognosis in patients with diverse cancers, especially …
-
Developmental Dynamics of 5-Hydroxymethylcytosine and its Role in the Terminal Differentiation of Neurons
… of cell division. Deletion of the 5hmC writers Tet1, Tet2, and Tet3 from postmitotic Purkinje cells alters cytosine modification in regulatory domains, impairs gene expression, hinders developmental transitions, and causes hyper-excitability and increased susceptibility to excitotoxic drugs. …
-
Epigenetic and Ubiquitin-Proteasome Mechanisms of Obesity Development
… to controls. Next, we used the CRISPR-dCas9-TET1 and dCas9-DNMT3a systems to test the role of Pomc DNA methylation in the hypothalamus in abnormal weight gain following acute exposure to a high fat diet in male rats. We found that exposure to a high fat diet increases Pomc DNA methylation and …
-
The Interaction of Early Growth Response Gene 1 and Myocyte Enhancer Factor 2C in the Murine Brain Cortex
… translocation methylcytosine dioxygenase 1 (TET1) to remove DNA methylation at target loci. Due to its critical functions during brain development and upon neuronal activation, mis-regulation of EGR1 is associated with neuropsychological disorders such as post-traumatic stress disorder (PTSD) …
-
Evaluation of Computational and Experimental Parameters in RNA Bisulfite Sequencing Analysis and Applications in Brain Development Studies
… the impact of m5C reader FMRP and m5C eraser TET1 loss in the mouse post-natal day 17 brain. Among a set of m5C sites identified in wildtype or knockout samples, few were differentially methylated after protein ablation, suggesting m5C may rely on compensatory enzymes. Using FMRP-RNA pulldown …
-
Multi-omics characterisation of mouse gastrulation and organogenesis at single-cell resolution
… DNA methylation defects in the blood lineage of Tet1/2/3 TKO chimeras. The results will reveal core functions of DNA demethylation during cell lineage specification and establishment of developmental competence. In sum, my PhD project provides a detailed study of the role of the spatial …
-
Regulation of De Novo and Maintenance Dna Methylation By Dnmt3A and Dnmt3B
… by deletion of the <em>Tet</em> genes (<em>Tet1/2/3</em>). Based on the results, I hypothesized that one or more 5hmC readers target DNMT3A/3B to hemi-hydroxymethylated CpG sites to prevent 5mC erosion. The hypothesis would predict that disruption of the 5hmC reader(s) in mESCs would exhibit …
-
Metabolomics and chemical library screening for antibacterial drug discovery
… regulation. TET-family dioxygenases (TET1, TET2 and TET3) initiate active demethylation of 5mC. TET2 oxidizes 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by iterative oxidation. Mutations in the TET2 gene are frequently detected in …
-
The role of Igf2 methylation and histone ubiquitination in age-related memory decline
… memory of aged rats, we used a CRISPR dCas9-TET1 system to oxidate DNA methylation marks at the Igf2 promoter, converting this region to a 5-hydroxymethylation (5-hmC) state that increase gene transcription. Remarkably, this manipulation led to improved memory retention in aged, but not …