Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"TCR sequencing"”.
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Characterisation of a public T cell clonotype enriched in m.tb infected individuals who do not progress to tuberculosis
… We recently completed a bulk T cell receptor (TCR) sequencing screen and observed that frequencies of a particular donor-unrestricted T cell (DURT) clone were higher in healthy, M.tb infected individuals who controlled infection (controllers), than individuals who progressed to tuberculosis …
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Decoding The Tumor and Immune Microenvironment In Pdac and Breast Cancer By Single Cell Sequencing.
<p>This work utilizes single cell RNA sequencing to identify transcriptional populations and gene changes for the purpose of immune-related cancer therapies. First we have characterized the T cell populations of the normal and malignant human pancreas. Furthermore, we utilized single cell TCR …
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Recovery of T cell receptor variable sequences from 3' barcoded single-cell RNA sequencing libraries
… In recent years, high-throughput single-cell RNA sequencing (scRNA-seq) has gained popularity among immunologists due to its ability to effectively characterize thousands of individual immune cells from tissues. Current techniques, however, are limited in their ability to elucidate essential …
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Mismatch Repair Deficient Neoantigen and Associated Circulating T-Cell Receptor Repertoires in Lynch Syndrome
… effectively. Furthermore, the T-cell receptor (TCR) landscape of LS patients, particularly those that recognize these neoags, has never been investigated. Thus, there is an excellent opportunity for exploring immune-based preventive strategies and gaining insights into the patterns of these …
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Bioinformatic tools for single-cell data analysis in clinical studies
… RNA-Seq, mass spectrometry, and single-cell sequencing However, the ability to gather exponentially more measurements comes with pitfalls of increased Type I error and reduced interpretability In theory, single-cell measurements can be helpful in combating this problem, since each sample of …
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Enrichment and Transcriptome Analysis of CD4+ T Cell Clones Harboring Intact HIV-1 Proviruses
… and constant domain of the T cell receptor (TCR) b chain, that are shared by all members of a given infected clone. The resulting enrichment enables the identification of the unique TCRab sequence of infected clones, which is presented in the second part. The TCRab sequence is a unique …
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DISSECTING IMMUNE DYSREGULATION IN PATIENTS WITHTHYMIC EPITHELIAL TUMORS BY AUTOANTIBODY ANDSINGLE-CELL PROFILING
… γδ and MAIT compartments, and single-cell RNA sequencing paired with αβ TCR sequencing to reconstruct developmental trajectories, functional states and clonal relationships. Pediatric thymuses were used as the healthy tissue counterpart whereas PBMCs from age-matched healthy donors were used as …
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Characterizing T Cell Features of Atypical Lymphocytes for the Optimization of Adoptive Immunotherapies
… success. For Tregs, the role of T cell receptor (TCR) specificity against disease-associated antigens is not clear due, in part, to a lack of comparative studies of TCR repertoires across diverse disease settings, which could inform which diseases would benefit from disease antigen-specific or …
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Subclonal Evolution of Chronic Lymphocytic Leukemia After Allogeneic T Cell Therapies
… alloSCT and DLI utilizing whole exome sequencing of purified CLL. Our cohort included 11 patients who relapsed after alloSCT, and we studied sequential samples in these patients to examine leukemic evolution. We identified clear patterns of linear and branched evolution in 8/11 patients …