Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"TCR repertoire"”.
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Quantitative dynamics of T-cell receptor repertoires and their use for cancer patient stratification
… specific way through their T-cell Receptors (TCR). As the genetic sequence encoding for the TCR is the result of somatic recombination, the TCR sequence varies from T-cell to T-cell, making it a functional barcode. The collection of TCRs present in an individual is known as a TCR repertoire, …
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Characterizing antigen-specific CD4⁺ T cells using HLA-DR oligomers
… engagement of their surface T cell receptors (TCR) by antigenic peptide bound to major histocompatibility complex (MHC). The success or failure of this TCR to MHC-peptide interaction determines the specificity of T cell action, and thus plays a central role in proper immune function. In this …
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Characterizing the role of thymic interferon in T cell development and selection
… cell selection, reduced T cell receptor (TCR) repertoire diversity and enhanced autoreactive T cell responses to self-antigens expressed during peripheral IFN signaling. Thus, constitutive exposure to interferons in the thymus is required for generating a safe and diverse TCR repertoire. …
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Investigating the longitudinal dynamics of the human gut microbiome and immune system
… of adaptive immunity, the T cell receptor (TCR) repertoire, in healthy individuals over a time span of up to one year. Our results served as the first reference point for normal temporal fluctuations in TCR repertoire dynamics. Together, this thesis underscores the value of modern …
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T-cell receptor diversity of various antigen-specific T cells
… a heterodimeric $\alpha\beta$ T-cell receptor (TCR). The TCR ligand is a peptide bound to a membrane-bound protein coded by the major histocompatibility complex (MHC) locus. Like immunoglobulins, the TCR repertoire is diverse because both the $\alpha$ and $\beta$ chains are coded by multiple …
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Role and specificity of regulatory T cells during retroviral infection
… Analysis of mice transgenically expressing the TCRβ chain of a FVspecific CD4+ T cell clone, which harbor a polyclonal TCR repertoire with increased frequency of FV-specific CD4+ T cells, indicated that the TCR repertoire of Treg cells in virus-naïve mice was virtually devoid of FV-specific …
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Autoimmune Susceptibility Imposed by Public TCRβ Chains
… It acts together with a corresponding TCR repertoire, yet, considering the extent of the repertoire's diversity, how this imposes disease susceptibility on a population is not well understood. We address the hypothesis that shared or public TCR, those present in most individuals, …
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Effects of Thymus Size and Involution On The Contribution of Recent Thymic Emigrants to The Peripheral T Cell Pool
… to maintain diversity of the T cell receptor (TCR) repertoire and produce immune responses against newly encountered antigens.</p> <p>The thymus involutes with age, after irradiation or chemotherapy, and due to severe viral infections. Thymus involution results in decreased thymopoiesis and RTE …
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Preventing Thymus Involution In K5.Cyclin D1 Transgenic Mice Sustains The Naïve T Cell Compartment With Age
<p>The thymus maintains T cell receptor (TCR) repertoire diversity through perpetual release of self-MHC restricted naive T cells. However, thymus involution during the aging process reduces naïve T cell output, leading to defective immune responsiveness to newly encountered antigens. We have found …
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Pathogenicity and Protection Mediated by a Single TCRβ in Experimental Autoimmune Encephalomyelitis
<p>How the TCR repertoire, together with risk-associated major histocompatibility complex (MHC), imposes susceptibility for autoimmune disease is not fully understood. A small fraction of TCR α or β chains are “public”, and are shared by most individuals.High-throughput sequencing of the mouse TCRβ …
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BACH2 orchestrates thymic T cell differentiation and functional programming
… which can be recognised by the T cell receptor (TCR) repertoire in order to protect from infection. Simultaneously, the thymus must ensure self-tolerance by pruning the repertoire of self-reactive cells, diverting these cells towards deletion or a regulatory fate. During thymic T cell …
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The roles of clustered membrane proteins in T-cell signaling
… clustered proteins such as the T-cell receptor (TCR) and Linker of activated T cells (Lat). Active ZAP-70 molecules, which phosphorylate tyrosine residues of Lat molecules, are generated from the clustered TCR molecules. Using spatial Gillespie simulation and mathematical modeling, I found that …
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Decoding Human T Cell Immunity With Single-Cell Multi-Omics
… T cell gene expression and T cell receptor (TCR) sequence modalities. Across several longitudinal infectious disease studies, I identify transient T cell states after infection and link these phenotypes with specific TCR sequences. In the first chapter, I provide a comprehensive overview of …
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T Cell Responsiveness Through the Ages: Functional Diversity of Age-Dependent Antiviral Responses
… infection also affected the γδ T cell receptor (TCR) repertoire by modulating the hierarchy of segment usage. Single-cell RNA sequencing with paired TCR repertoire data from the lungs showed that both age and MCMV infection impacted γδ T cells in a manner determined by age of viral exposure, …
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Exploring the Functional Role of Helios in Mucosal Associated Invariant T Cells using siRNA.
… can display an almost unlimited T cell receptor (TCR) repertoire, the specificities of MAIT cell TCRs are limited. This evolutionarily conserved subset displays a semi-invariant TCR which recognises riboflavin metabolites, produced by a wide range of bacteria and fungi, and presented on the major …
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Development of a Neoepitope Prioritization Platform to Identify Immunogenic Neoantigens from Oncogenic FGFR3-TACC3 Fusion in Glioblastoma
… Integration of single-cell transcriptomic and TCR repertoire analyses revealed that peptide stimulation induces coordinated activation of immune programs, including T cell activation, cytokine signaling, proliferation, and metabolic pathways. The best peptide pool elicited increased abundance …
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The Influence of the Microbiota on T Cell Development
… thymus, including shaping the T cell receptor (TCR) repertoire in thymocytes. The impact of the microbiota on T cell development, however, still remains an understudied area of research. There has also been no published data exploring the effects of the microbiota on RTE phenotypes and …
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The Role of TCR Specificity in the Regulation of the Immune Response to Self and Commensal Antigens
ABSTRACT OF THE DISSERTATION The Role of TCR Specificity in the Regulation of the Immune Response to Self and Commensal Antigens by Stephanie K. Lathrop Doctor of Philosophy in Biology and Biomedical Sciences: Immunology) Washington University in St. Louis, 2010 Dr. Chyi-Song Hsieh, Chairperson The …
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Roles of CTCF and YY1 in T Cell Receptor Gene Rearrangement And T Cell Development
<p>Diversity of T cell receptors (TCR) and immunoglobulins (Ig) is generated by V(D)J recombination of antigen receptor (AgR) loci. The Tcra-Tcrd locus is of particular interest because it displays a nested organization of Tcrd and Tcra gene segments and V(D)J recombination follows an intricate …
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A Clonal, Transcriptomic, and Functional Analysis of T-cells Mobilized to Blood in Response to Acute Exercise
… Therefore, assessment of the T-cell receptor (TCR) repertoire, is vital to deepen our understanding of the beneficial effect of acute exercise on immune cell phenotype and function. Furthermore, utilizing multimodal sequencing analysis would elucidate the transcriptomic changes induced by acute …
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