Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"TCR Signalling"”.
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IDENTIFICATION OF CDC7 AS A NEW REGULATOR OF T CELL ACTIVATION
… was designed to identify novel mediators of TCR signalling. A library of kinase inhibitors was screened for their effects on suppressing activation in several T cell populations. The screening assay was able to identify inhibitors of canonical kinases of TCR signalling and kinases that were …
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How TCR signal strength controls CTL polarisation for target killing
… of the clonally expressed T cell receptor (TCR). TCRs recognise a specific peptide chain loaded into a major-histocompatability complex, triggering signalling, inducing the CTL to attach and kill target cells. Key stages in this attack are the initial conjugation followed by polarisation and …
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BACH2 orchestrates thymic T cell differentiation and functional programming
… which can be recognised by the T cell receptor (TCR) repertoire in order to protect from infection. Simultaneously, the thymus must ensure self-tolerance by pruning the repertoire of self-reactive cells, diverting these cells towards deletion or a regulatory fate. During thymic T cell …
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Development of an Optogenetic Toolkit for the Interrogation of T cell Signalling Dynamics
… approaches for the modulation of T-cell signalling dynamics and the interrogation of key events in T-cell activation to help investigate this question. Optogenetics is a rapidly emerging technique whereby light can be used to control the spatial and temporal activation, or inactivation of …
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The role of Hedgehog signalling in T cell effector function
… such as infection and cancer. The Hedgehog (Hh) signalling pathway has long been known to play a critical role in embryonic development and adult tissue maintenance. Recently, Hh signalling has been shown to be important for CD8+ T cell function downstream of T cell receptor (TCR) signalling. It …
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Elucidating the molecular mechanisms of p110δ activation in T cell antigen receptor signalling
… Engagement of the T cell antigen receptor (TCR) initiates a signalling cascade leading to the rapid induction of PI3K activity. The class IA catalytic isoform p110δ is known to be the dominant PI3K involved in signal transduction downstream of the TCR. However, the mechanism by which the TCR …
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Constraints on the CD8 T cell response in chronic HBV infection
… ability to produce IL-2 and proliferate upon TCR dependent stimulation. By contrast, these CD8 T cells had preserved production of the proinflammatory cytokines IFN-γ and TNF-α. We provide evidence that this global CD8 T cell impairment of IL-2 production and proliferation may be partially …
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Regulation of inflammatory TLR signalling by the BCAP adaptor
… a PI3K adaptor protein in TLR, BCR and IL-1R and TCR signalling. In TLR signalling, BCAP acts as a negative regulator of inflammatory signalling. Regulation of TLR signalling is essential as overstimulation can cause excessive and pathological inflammation, which leads to serious illnesses such as …
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TRP channels and a dextran sulfate activated rise in intracellular Ca2+ and Na+ in a subpopulation of double positive thymocytes
… characterisation of this rise and its underlying signalling and conductance is pivotal. I have used flow cytometry to concomitantly image [Ca2+]i and [Na+]i after DP thymocytes were exposed to 1 ug/ml DxS (500 kDa) using AM-loading of the indicators indo-1 and ANG-2 or SBFI (5 uM for all). In some …
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TRP channels and a dextran sulfate activated rise in intracellular Ca2+ and Na+ in a subpopulation of double positive thymocytes
… characterisation of this rise and its underlying signalling and conductance is pivotal. I have used flow cytometry to concomitantly image [Ca2+]i and [Na+]i after DP thymocytes were exposed to 1 ug/ml DxS (500 kDa) using AM-loading of the indicators indo-1 and ANG-2 or SBFI (5 uM for all). In some …
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PKB/Akt : a critical regulator of lymphocyte, development and function
… enhanced proliferation after T cell receptor (TCR) engagement compared to wild type (wt) mice. Astonishingly, myr PKB tg thymocytes were capable to proliferate in response to PMA only and were also less sensitive to inhibition by the calcineurin inhibitors CsA or FK506, which indicates the …
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Using single-cell RNA sequencing to elucidate the biological basis of IBD and response to therapy
… and enriched pathways related to cytokine signalling and the citric acid cycle. Colocalisation analysis using updated CD genome-wide association summary statistics from our lab showed that the disease-associated variant likely decreased the expression of ADCY3 in immune cell subsets …
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A novel antigen-receptor signalling reporter mouse reveals clonotype-independent intratumoral trapping as a spatial feature of CD8+ T cell exhaustion
… a novel mouse model, termed Antigen-Receptor Signalling Reporter (AgRSR) mouse, that uses the specificity of Nur77 (NR4A1) for antigen receptor activation to drive expression of a tamoxifen-inducible CreERT2, enabling time-stamping of TCR-activated lymphocytes with EYFP expression through a …
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Regulation of LFA-1 on T cells by phosphoinositide signalling
… following chemokine and T cell receptor (TCR) engagement by inside-out signalling. This process coordinates T cell migration, adhesion, and activation of T cells. LFA-1 activation is mediated by phosphoinositide 3-kinase (PI3K) and the downstream phosphoinositide PtdIns(3,4,5)P3 (PIP3). To …
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Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment
… by a series of checkpoints in which the signalling capacity of the T-cell antigen receptor (TCR) is tested. One such checkpoint is the generation of the clonotypic chains of the two TCR variants (αβ-TCR or γδ-TCR) by V(D)J recombination. Importantly, the TCRα chain is rearranged only …