Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"TCM-199"”.
-
Effect of different culture media and incubation methods on culturing murine embryos in vitro using a semen straw as an alternative receptacle
… and compare the in vitro embryo development in TCM-199 and Ham's F10 culture media until the blastocyst formation. The spermatozoa and oocytes of an F1 generation (Balb C x C57) were fertilized in Ham's F10 and TCM-199 in semen straws and in micro-drops in petri dishes. The embryos were cultured …
-
Comparison of two different media and assisted hatching techniques on the embryo hatching rate using the mouse as a model
… media ((Ham’s F10 and Tissue Culture Medium 199 (TCM-199)) and four assisted hatching techniques (mechanical, chemical, enzymatic and laser) on blastocyst formation and hatching rate using murine embryos as a model. The C57BL/6 and BALB/c mouse breeds were bred and raised until they reach …
-
Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… one-cell bovine embryos were cultured in TCM-199 (n=46), co-cultured with bovine oviductal epithelial cells (OEC; n=38), or in explanted immature mouse oviducts (n=54). Of the embryos that cleaved once, 52.6, and 30.4 and 0.0% developed to morulae/blastocysts after culture in OEC, TCM-199, …
-
Maduración in vitro de ovocitos de perra provenientes de folículos poliovocíticos
… se extrajeron y se incubaron por 72h en TCM-199 suplementado bajo condiciones de cultivo. Posterior al cultivo se evaluó el desarrollo nuclear de los ovocitos previa fijación y tinción nuclear DAPI mediante microscopía de epifluorescencia. El desarrollo meiótico se clasificó en vesícula …
-
Influence of Growth Factors on Bovine Embryo Development
… from slaughterhouse ovaries were matured in TCM-199 (control), with or without the addition of 0.6 mM cysteine (C) at 0 or 12 h of maturation. After in vitro fertilization, embryos were allocated to culture treatments containing synthetic oviductal fluid medium. Culture treatments included …
-
Assistierte Reproduktion beim Pferd
… maturation. After 30 hours of incubation in TCM 199, the maturation rate of equine oocytes to metaphase II is up to 80 % . In vivo fertilization of oocytes can be achieved by surgical transfer into the oviduct or follicles of inseminated recipient mares. The procedure of gamete intrafallopian …