Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"TCF/LEF"”.
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Interactions between BMP and Canonical Wnt Signaling Regulate Critical Stages of the Osteoblast Lifecycle
… a lineage allocation mechanism requiring both Tcf/Lef-dependent and Tcf/Lef-independent mechanisms. We next examined BMP/Wnt interactions during proliferation of osteoprogenitors, by focusing on Smad4, a central mediator of the greater TGF-beta/BMP pathway. Acute, tamoxifen-induced deletion of …
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Mechanistic Dissection of Tcf7l1-Dependent Enhancer Decommissioning During Exit from Naïve Pluripotency
… to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at Tcf7l1-bound cis-regulatory elements in mouse pluripotent stem cells. A combination of gene knockout and inducible expression lines of ESC were generated to …
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COMPARATIVE CELL BASED FUNCTIONAL ANALYSIS OF KEY WNT/BETA-CATENIN PATHWAY GENES AND GENE MUTATIONS
… transcription of its target genes by binding TCF/LEF transcription factors. Aberrant Wnt/Beta-catenin signaling activity leads to misregulation of proliferation and metabolic pathways and has been linked to a number of diseases.
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Wnt-/beta- Catenin Zielgene der frühen Mausentwicklung
… Interaktion des transkriptionellen Komplexes aus Lef-/ b- Catenin mit einem brachyury- Promoterfragment gezeigt und in Transaktivierungsexperimenten, die Relevanz von Tcf-/Lef- Bindungsstellen innerhalb des Promoters nachgewiesen worden. <br>Zur Identifizierung weiterer Wnt- regulierter Gene, …
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TCF4-mediated Transcriptional Regulation of Krüppel-like Factor 4 Gene
… 결정을 드러내도록 노력하기 위해 우리는 transcription factor 4 (TCF4) 단백질이 binding 하는 것으로 알려진 두 개의 AT-/TA-CAAAG conserved motif를 확인하였다. Transcription factor 7-like 2(TCF7L2)로 알려진 TCF4은 transcription factor의 하나이고, 성인의 장에서 Wnt/β-catenin 신호 전달 활동을 중재하고, 결장 표면에서 발현되는 primary TCF/LEF family member이다. KLF4 유전자의 변화활동이 …
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Die Interferenz des Tumorsuppressor-Homologen p63 mit dem kanonischen Wnt-Signalweg
… eine Interaktion von ΔNp63α mit Mitgliedern der TCF/LEF-Transkriptionsfaktoren des kanonischen Wnt-Signalwegs nachgewiesen. Diese Beobachtungen sind vereinbar mit einem Modell, in welchem ΔNp63α als Brückenprotein Transkriptionsrepressoren an die Promotoren Wnt-responsiver Gene rekrutiert. Auf …
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Delineating the molecular mechanisms regulating chondrogenesis
… molecules in the regulation of chondrogenesis. TCF/LEF-LacZ reporter mice show activated canonical WNT signaling distributed throughout the embryonic age (E) 9.5 forelimb. At later stages, LacZ expression becomes confined to distal regions of the limb bud. Previous studies have demonstrated that …
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Role of ß-catenin and Leukotriene in Colon Cancer Progression
… phosphorylation of GSK-3β and activation of Tcf/Lef. Furthermore, LTD4 decrease both membranous E-cadherin and β–catenin which lead to an increase migration of HCT116 colon cancer. I also found that membrane expression of β–catenin is associated with good prognosis while nuclear GSK-3β is …
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Regulation of Wnt signalling in ovarian cancer and immune cells
… In this thesis, I investigate the expression of TCF7 and the less studied TCF7L2, both Wnt transcription factors of the TCF/LEF family, in different immune subsets. I focus on the detection and the differential expression of extended and short isoforms of these transcription factors and find …
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Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
… cells. Finally, I examined the expression of the TCF/LEF transcription factors in human pluripotent and differentiated cells. Human naïve ES cells fail to express any of these factors but primed ES cells readily express TCF7L1 and TCF7L2. Trophoblast-like cells robustly express TCF7L2 and …
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TOWARDS RETINA REGENERATION: THE EPIGENETIC BASIS OF NEURONAL AND AXON GUIDANCE GENE REGULATION
… EphA5 proximal promoter contains four predicted TCF/LEF binding sites, suggesting a potential role for WNT signaling in the transcriptional regulation of EphA5. In luciferase assays, activation of the canonical WNT signaling pathway increased the activity of mouse EphA5 promoter constructs in …
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Investigating the Non-globular Proteins of the Canonical Wnt Signalling Pathway
… transported to the nucleus where it binds to the TCF/LEF family of transcription factors, inducing the expression of cell cycle promotor genes. In this Thesis I describe investigations into the roles of leucine-rich repeat kinase 2 (LRRK2) and the transcription factor TCF7L2 within this signalling …
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Investigating the functional significance of the upregulation of Cyclin D2 and p21 following Apc loss in vivo
… of the Wnt pathway and the activation of TCF/LEF target genes. This project focuses on the proto-oncogene c-Myc as it is a key Wnt target gene which is activated following loss of Apc in vivo. This upregulation is noteworthy as c-Myc is implicated in stem cell survival, proliferation, …