Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 99 for “"TCA cycle"”.
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The Tca Cycle As A Nexus of Metabolic Vulnerabilities In Cancer
… mitochondrial metabolism (tricarboxylic acid cycle (TCA) and oxidative phosphorylation) is imperative for cancer cell growth and proliferation. The scope of the works presented in this dissertation is to explore the importance of mitochondrial metabolism, and primarily the TCA cycle—the …
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Energy metabolism in Arbidopsis thaliana: TCA cycle evolution, amino acids degradation and alternative pathways
… heritage is related to the tricarboxylic acid (TCA) cycle. The TCA cycle is an essential pathway which is related to reducing power (NADH and FADH2) generation, nitrogen assimilation and photosynthesis optimization. It has been suggested that the TCA cycle operated as isolated steps prior …
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The effect of beta-oxidation or TCA cycle inhibition on mitochondrial function and the sensitivity of high resolution respiratory detection
… metabolism do not account for defects in the TCA cycle and oxidative phosphorylation. These metabolic pathways are connected and dysfunction in one pathway (primary) could lead to dysfunction in the other (secondary). We propose the use of methods that combines all aspects of the bioenergetics …
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Investigation of Gut-Derived Circulating Succinate in Metabolism and Liver Signaling
<p>The tricarboxylic acid (TCA) cycle is the epicenter of cellular aerobic metabolism and accounts for the majority of cellular ATP production. In addition to its use in anabolic and catabolic processes, TCA cycle intermediates may serve as signaling molecules. The TCA cycle intermediates succinate …
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Metabolic flux, transport activity, and subcellular communication in intact hearts from dynamic ¹³C NMR spectroscopy
… or without KCl arrest. The tricarboxylic acid (TCA) cycle flux and the interconversion rate between (-ketoglutarate and glutamate were determined by least-square fitting of a kinetic model to NMR data. Rates were compared to flux through glutamate-oxaloacetate transaminase (GOT). Despite similar …
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Interrogating and potentiating energy metabolism in the human brain after traumatic brain injury
… glucose, lactate and tricarboxylic acid (TCA) cycle metabolism. Study I: I defined the optimal parameters for quantitative proton and carbon-13 NMR of cerebral microdialysates. Study II: I measured baseline microdialysate metabolite concentrations for brain and muscle and investigated the …
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Mathematical modelling of mammalian mitochondrial metabolism
… The mammalian liver-specific tricarboxylic acid cycle (TCA) model developed within the Cell Systems Modelling Group (CSMG) was used to study and understand the basic functional properties of a tissue-specific TCA cycle model system and to form a knowledge base. Based on this knowledge, we …
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The growth, physiology and intermediary metabolism of bacillus stearothermophilus.
… whether a functional tricarboxylic acid (TCA) cycle was present in vegetative cells of B. stearothermophilus, and to understand the effects of growth conditions on the carbohydrate metabolism and cytochrome content of the organism. In order to study the intermediary metabolism, the …
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Non-Canonical Roles of Cytosolic Phosphoenolpyruvate Carboxykinase in Small Intestine Metabolism
… to glucose, triglycerides, amino acids, and TCA cycle metabolism using a novel everted intestine perfusion model, isolated enterocytes, 13C stable isotope tracers, GC-MS, LC-MSMS, 13C NMR spectroscopy, and mathematical modeling. The functions of PEPCK in small intestine enterocytes largely …
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Metabolic engineering of microorganisms for the overproduction of fatty acids
… strain. MFA identified the up-regulation of the TCA cycle and down-regulation of pentose phosphate pathway under fatty acid overproduction to replenish the need of energy and reducing molecules. The elevation of intracellular metabolite levels in the TCA cycle complemented the flux findings. With …
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The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
… require oxygen, iron and the Tricarboxylic Acid (TCA) cycle metabolite, 2-oxoglutarate (2-OG/a-ketoglutarate) for catalytic activity. Therefore, understanding how 2-OG levels are regulated is essential to dissect the relative contribution of metabolism to activation of HIFs. A key determinant of …
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Quantifying the Intracellular Metabolic Network that Establishes the Simultaneous Utilization of Sugars and Aromatic Substrates in Pseudomonas putida KT2440
… almost exclusively to the tricarboxylic acid (TCA) cycle. I found that benzoate catabolism influenced the routing of glucose in the ED pathway, reverse EMP pathway, and PP pathway, despite the absence of benzoate-derived carbon in these pathways. In the TCA cycle, the simultaneous catabolism of …
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The Mitochondrial Fusion Protein, Optic Atrophy 1 (OPA1), Alters Cellular Metabolism to Support Cancer Cell Viability
… further coordinates with the tricarboxylic acid (TCA) cycle. To study mitochondrial fusion as a target, we found that silencing of OPA1 in TNBC models led to suppressed growth in anchorage-independent soft agar and tumour xenograft experiments. OPA1 silencing disrupted cristae formation and …
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The Role of Pyruvate Dehydrogenase in Cell Growth
… derived carbon into the tricarboxylic acid (TCA) cycle. To test whether cells require glucose oxidation for proliferation, the work in this thesis establishes a model wherein PDH activity is suppressed using RNA interference. Small hairpin RNAs against the transcript encoding the PDHE1α …
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Deciphering Metabolic Adaptations in Salmonella Enterica Serovar Typhimurium 14028S During Batch Growth and Gentamicin Exposure Using Absolute Quantitative Proteomics
… results in harmful events, such as futile cycles, activation of toxic pathways, and accumulation of harmful by-products that cause bacterial growth inhibition and death. However, these events remain only partially understood and evidence suggests an intricate interplay between metabolic …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… of fluxes among the tricarboxylic acid (TCA) cycle, pentose phosphate pathway, pyruvate node, alpha-ketoglutarate node and oxaloacetate node changed. By comparing the metabolic flux maps of E. coli MG1655 grown at different conditions, pathways that have flux change under stress were …
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Glutamine Antagonism and Its Utlity [sic] as a Therapeutic Modality in Cancer
… is important in cancer as it fuels the TCA cycle, plays a role in redox homeostasis, and contributes to the production of nucleotides, amino acids, and lipids for survival, making glutamine metabolism a promising target in cancer therapy. The work outlined in this dissertation focuses on …
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Methodologies and analysis of metabolic flux in mammalian systems
… metabolism to pentose phosphate pathway and TCA cycle. In isotope-infused mice, the new MFA framework directly measured systemic glucose and lactate contribution to TCA cycle under physiological condition, which confirms the conventional knowledge that glucose is the main direct energy source …
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Toxicity and biotransformation of 1,1,1,3,3-Pentafluoropropane, 3,3,3-Trifluoropropionic acid and 1,1,1,3-Tetrachloropropane
… an imbalance between glycolysis, lipolysis, TCA cycle activity and respiratory function. Based on the results, ß-oxidation of fatty acids was not affected, and due to the decrease in glucose levels and the high levels of acetyl CoA, glycolysis was considered not to be impaired. Increased …
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Functional and mathematical analysis of the glyoxylate shunt in Streptomyces coelicolor
… aceA, ccr and genes encoding tricarboxylic acid (TCA) cycle genes revealed that all are subject to a similar level of purifying selection pressure. The operons of the glyoxylate shunt and the ethylmalonyl-CoA pathway share a 15 bp palindromic motif in their upstream sequences, which was also found …
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