Global ETD Search
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Showing 1 to 7 of 7 for “"TAT-CaM"”.
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Utilization of the Cell Penetrating Peptide TAT-CaM in New Biological Systems: Fungal and Bacterial Cell Lines
… this phenomenon is explored using the CPP TAT-CaM in both non-pathogenic and pathogenic strains of fungal and bacterial cell lines. In this study, it was demonstrated that the CPP TAT-CaM is nontoxic to all cell lines used with a viability of over seventy-five percent. It was also …
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Novel use of a cell-penetrating peptide-adaptor system to characterize Akirin constructs and investigate activity of bacterial effector proteins
<p>In the late 1980s, HIV protein TAT was found to confer the passage of HIV through the plasma membrane of mammalian cells. The McMurry lab developed a CPP-adaptor system which consists of the fusion of TAT to calmodulin to which mediates the delivery of exogenous proteins into cells and prevents …
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Simultaneous Delivery of CRISPR/Cas Complexes and Donor DNA Using Cell-Penetrating Peptide-Adaptors
… to treat many human diseases caused by these mutations. Research using CRISPR/Cas systems in the treatments of many of the genetic diseases has resulted in misfortunes (such as toxicity to the patient or new diseases) because of the method of delivery such as viral delivery. </p> <p>The current …
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USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
… undesirable off-target effects, e.g. random mutations in the genome. A safer approach to inducing pluripotency is exogenous delivery of OSKM proteins using cell-penetrating peptides (CPPs). Classically, CPP-mediated delivery has been inefficient because of poor penetration and entrapment in …
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Delivering Signal-Altering Bacterial Effector Proteins to Mammalian Cells Using Cell-Penetrating Peptide Technology
… peptide (CPP) delivery system called TAT-CaM. Here, we show that the TAT-CaM system is capable of delivering YopJ into mammalian cells and that YopJ is capable of inducing cell death once delivered. Although we encountered issues with reproducibility, we believe that TAT-CaM-YopJ could …
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I) Hv1, LBP and LCF reconstitution in Liposomes II) Signed, Sealed, and Delivered: CPP Technology Delivers Active Protein Cargo
… on cell penetrating peptide (CPP) technology. TaT-CaM is a recently developed CPP that crosses cellular membranes and carries a wide variety of proteins. This study focuses on post-delivery effects of catalase, a peroxidase protein that breaks down H<sub>2</sub>O<sub>2</sub>, as proof of …
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Development of a Real-Time Cell Imaging Assay to Characterize the Delivery of a Cell-Penetrating Peptide Adaptor and its Associated Cargo
… of endocytosis. To combat this issue, a new CPP, TAT-CaM, that can reach the cytosol has been developed. Conventional methods involve observing these peptides in a static manner, which has hindered a complete understanding of CPPs. Therefore, a real-time imaging assay was developed to characterize …