Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"TAT peptide"”.
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Characterization of anti-DNA antibody with Tat peptide sequence in CDR
"Tat 펩티드가 삽입된 항-DNA 항체 구조의 생화학적 특성 항-DNA 항체인 3D8 단일 가닥 가변부위 (single chain variable fragment: scFv)는 핵산결합능 및 가수분해활성이 있으며, 3D8 VL 단독부위만으로도 핵산 결합능 및 가수분해능을 발휘한다. 또한 3D8 scFv는 세포막 침투능이 있어서, 세포막을 투과한 후, 세포질에 위치하게 되고, 결국은 세포사멸을 유도한다고 알려져 있다. 본 연구에서는 3D8 scFv의 핵산가수분해 활성은 그대로 유지하면서, 세포막 투과 후 핵으로 이동할 수 있는 …
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Structural transitions in self-assembled lipid systems driven by induced curvature: from cell-penetrating peptides to programmable vesicles
Arginine rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still …
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Intracellular delivery of rabbit monoclonal antibody
<p>In the past decades, a series of small peptides, Protein Transduction Domain (PTD), were discovered to be able to facilitate the delivery of small proteins into living cells. With the specific feature, researchers have successfully delivered some functional proteins into living cells. To fully …
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Structural Transitions in Self-Assembled Lipid Systems Driven by Induced Curvature: From Cell -Penetrating Peptides to Programmable Vesicles
… of other arginine rich cell-penetrating peptides, including Antp and oligoarginine, with model cell membranes, and find that the transduction activity correlates with induction of negative Gaussian curvature. The negative Gaussian membrane curvature is broadly enabling and its induction …
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Synthesis and Cytotoxicity Studies of Gold Nanoparticle Systems
… the introduction of the bivalent hexadentate Ni(II) onto the NTA matrix of the MPCs, were conducted to formulate the biomolecular hybrid systems. A range of biomolecules including the cell-penetrating TAT peptide (YGRKKRRQRRR), mitogen-activated protein kinase (MAP kinase), streptavidin …
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Solid Phase Synthesis and Biological studies on metal conjugates of bioactive peptides for targeted delivery
Summary Peptide-based drug delivery systems and therapeutics have gained an enormous attention during the last ten years. Progress in this field will lead to site specific delivery, improved receptor affinities, efficient cellular uptake and/or nuclear targeting. Thus, in this research project, …
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REGULATED UPTAKE OF BIOPOLYMERS Role of cell surface proteoglycans Implications for drug and gene delivery
… bacterial toxins and viral proteins. The HIV-1 TAT protein was shown to enter cells and to target their nuclei, thus acting as a paracrine transcription factor, a finding that initiated the field of so called cell penetrating peptides (CPPs). Due to their ability to efficiently deliver …
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On the Role of Polyamines and Microvesicles in Tumour Development. Regulation by Hypoxia and Implications for Therapeutic Intervention of Cancer.
… by a HIV-1 trans-activator of transcription (TAT) peptide, Tat, results in reduced tumour growth. We found a novel role for the polyamine system in the hypoxia-induced adaptive response of tumour cells; depletion of polyamines sensitizes tumour cells to hypoxic stress and enhances the tumour …
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Properties and biomedical applications of magnetic nanoparticles
… coated iron oxide nanoparticles labeled with tat peptide to the cell nucleus, which permits this system to be used for a variety of biomedical applications.</p> <p> Beyond investigating magnetic nanoparticles for biomedical applications, we also studied their magnetohydrodynamic and dielectric …
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Recombinant production of Aβ1-42 peptide and analysis of interactions between Aβ1-42 and Peptide Inhibitor NanoParticles (PINPs) developed as a potential novel treatment for Alzheimer’s Disease
… addressed two main aims relating to the Aβ1-42 peptide – widely demonstrated to play a key role in the pathogenesis of Alzheimer’s Disease (AD). Aβ1-42 is an amyloid peptide that readily self-associates forming neurotoxic aggregates. With the primary risk factor for AD being age, and ageing …
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Synthesis and Characterization of Miniaturized Fluorescence Sensors for Aqueous and Cellular Measurements
… particle-based sensors with cell penetrating peptides to enable their permeation into cells. This step is essential for the use of particles as intracellular sensors. Streptavidin coated microspheres were modified by the strongest noncovalent interaction between avidin and biotin. Tat peptide …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… the activities of 3D8 antibody using synthesized peptides corresponding to 3D8-CDRs. Only pep-L1 bound to DNA or heparin, however pep-L1 did not enter the HeLa cells. Pep-H1 and pep-H2 entered the HeLa cells, even though much lower penetration efficiency than Tat peptide. Consequently, our results …
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17-AAG and sihsp90α combinational therapy as a novel anti-cancer approach
… EGFR, CDK and PDGFR. These client proteins are mutated or overexpressed in tumours and are involved in tumour progression and metastasis due to their roles in signalling pathways, cell cycle and apoptosis. Hsp90 has two isoforms, namely Hsp90α and Hsp90β and share 85% sequence homology. Hsp90β is …