Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"TAR RNA"”.
-
Nuclear magnetic resonance studies of HIV TAR RNA mutants
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
-
Structure and dynamics of the HIV-2 TAR RNA-argininamide complex by NMR spectroscopy
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1998.
-
NMR Studies on Termination/- Antitermination Complexes of HIV-1 Transcription
After HIV enters the host cell the viral RNA is reverse transcribed into double stranded DNA and then integrated into the host genome. At this step, transcription of HIV RNA by Rpol II is tightly regulated by several cellular factors including NELF, DSIF, and pTEFb, and the virus encoded Tat …
-
In vitro-Selektion am lentiviralen Transaktivator-Protein aus HIV-1
… Bindung von phagenpräsentiertem HIV-Tat an HIV-TAR-RNA erfolgt im Phagen-Display nur mit geringer Affinität. Durch die Erweiterung des Tat-TAR-Phagen-Displays mit dem zellulären Kofaktor Cyclin T1 konnte die Affinität und Spezifität der Bindung von HIV-Tat an HIV-TAR-RNA gesteigert werden. Somit …
-
Strukturelle Untersuchung des Antiterminationskomplexes von EIAV
… des Proteins bei der Wechselwirkung mit TAR-RNA, Cyclin T1 und dem Inhibitor TF53 auf der Basis einzelner Aminosäuren direkt in einem 15N-1H-HSQC-Spektrum beobachtet werden. Es konnte gezeigt werden, dass das EIAV-Tat-Protein ohne die Beteiligung weiterer Bindungspartner an die TAR-RNA …
-
Warten auf die Transkription: Die humanen Elongationsfaktoren NELF und DSIF
… Dabei interagieren beide Faktoren direkt mit RNA-Polymerase II. NELF bindet über die RNA recognition motif (RRM)-Domäne der Untereinheit E zusätzlich an die naszierende RNA. Eine derartige Transkriptionsblockade tritt auch während der Transkription des Genoms des humanen Immunschwächevirus 1 …
-
Recombinant Expression, Purification, and Characterization of an HIV-1 Tat-Human Cyclin T1 Chimera
… protein Tat with the trans-activation response (TAR) stem-loop of the long-terminal repeat (LTR) portion of nascent viral RNA. The production of viable transcripts is enhanced dramatically by the interaction of HIV-1 Tat with the host protein human Cyclin T1. Interaction with hCycT1 remodels Tat …
-
Enhancement of Dicer as a personalized anticancer strategy
Normal miRNA production is essential for regulating protein expression and cellular fate. Misregulation of miRNA production is emerging as a key factor in the development of many disease states, including most forms cancer. A global downregulation of miRNA processing is common among cancers and has …
-
Targeting HIV-1 RNAs with Medium Sized Branched Peptides Featuring Boron and Acridine-Branched Peptide Library Design, Synthesis, High-Throughput Screening and Validation
RNAs have gained significant attention in recent years because they can fold into well-defined secondary or tertiary structures. These three dimensional architectures provide interfaces for specific RNA-RNA or RNA-protein interactions that are essential for biological processes in a living system. …
-
Regulation of Pkr Activation by Pact and Trbp In Response to Stress Signals
<p> PKR (protein kinase, RNA activated) is an interferon (IFN)-induced serine-threonine protein kinase, which plays a crucial role in IFN's antiviral and antiproliferative actions. In addition, PKR plays a regulatory role in signal transduction leading to apoptosis in response to a variety of …
-
Study of macromolecular interactions using computational solvent mapping
… DNA with formaldehyde. Extending the method to RNA molecules, I applied the FTMap algorithm to flexible structures of HIV-1 transactivation response element (TAR) RNA and Tau exon 10 RNA. Results show that despite the extremely flexible nature of these small RNA molecules, nucleic acid bases …