Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"TALENs"”.
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease. Subsequent repair of …
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… several pairs of TAL effector nucleases (TALENs) designed to target exon 1 of ADA. These TALENs function as dimers, and each pair creates a different targeted double strand break near the start site of the ADA gene. The most effective pair induces a DNA double strand break immediately …
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Cellular reprogramming in eukaryotes
… activator-like effector (TALE) nucleases (TALENs), which allows researchers to design the DNA binding sequence through a simple cipher. In one example, we will discuss the development of a liquid phase high-throughput TALE synthesis platform (fairyTALE) capable of producing TALE-nucleases, …
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The role of transcription factor GATA6 in the development of the human pancreas
… The use of site-specific nucleases such as TALENs for such a purpose represents a paradigm shift in disease modelling, where TALENs are capable of directly correcting disease-causing mutations, therefore permanently eliminating the symptoms with precise genome modifications. Alternatively, …
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Gene editing in Aedes aegypti
… repair HE-induced dsDNA breaks. TALE nucleases (TALENs) are a group of artificial enzymes capable of generating site-specific DNA lesions. To examine the ability of TALENs for gene editing in Ae. aegypti, a pair of TALENs targeted to the kmo gene were expressed from a plasmid following embryonic …
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Genetic strategies to manipulate meiotic recombination in Arabidopsis thaliana
… into crossovers. Instead, TALFokI nucleases (TALENs) were expressed from meiotic promoters in order to generate exogenous DSBs at sites determined by the DNA binding specificity of the TAL repeat domains. The project succeeded in transforming TALENs into spo11-1 mutants and confirming their …
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Modeling 3D Retinogenesis in Mouse Embryonic Stem Cells Following CRISPR-mediated Crx Knockdown
… Transcription Activator-like Effector Nucleases (TALENs).</p> <p>In order to overcome current limitations on disease-specific model systems, we developed a protocol for differentiating mouse embryonic stem cells into 3D retinas <em>in vitro</em>. In addition, to confirm the utility of this 3D …
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Characterization of NHLRC2 gene-edited mice: a model for bovine developmental duplications
… Transcription activator-like effector nucleases (TALENs) targeting exon 5 of NHLRC2 were used for gene-editing of the orthologous locus in mice to further investigate the role of NHLRC2 in development. Three mouse lines were generated with mutations having varying impacts on the NHLRC2 protein. …
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LncRNA discovery in the Listeria monocytogenes infection model
… using transcription activator-like nucleases (TALENs) to validate their candidacy as infection-regulating lncRNAs. Preliminary investigations conducted in this study have revealed potential Listeria infectionregulating lncRNA candidates. Furthermore, we explored the use of the physiologically …
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Generation of a Medaka Fish Model of Propionic Acidemia for Development of Novel Therapies.
… in medaka fishes (<i>Oryzias latipes</i>) using TALENs targeting the <i>pccb</i> medaka gene, encoding one of the subunits of PCC. The <i>pccb<sup>del/del</sup></i> medaka recapitulates the clinical and biochemical phenotype of human patients. Affected medaka larvae showed early lethality and …
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A Bioethical Paradigm for Enhanced, Post or Transhumans in Medicine and Biological Research
… defects in potential offspring. CRISPR-Cas9, TALENs, cloning and haploid stem cells are the technologies that not only can be used to develop specific treatment mechanisms, but they are the mechanisms that will allow humankind to engineer new kinds of persons, thus creating transhumans. From …
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Genetic Correction of Duchenne Muscular Dystrophy using Engineered Nucleases
… Transcription activator-like effector nucleases (TALENs) were engineered to mediate highly efficient gene editing after introducing a single TALEN pair targeted to exon 51 of the dystrophin gene. This led to restoration of dystrophin protein expression in cells from DMD patients, including …
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Investigating Mechanisms of Telomere End-Protection
… of human POT1 in telomere protection, we used TALENs to generate human knockout cell lines lacking POT1. We found that similar to mouse POT1, deletion of human POT1 elicited significant DNA damage signaling. Strikingly, the amount of 3' singlestranded DNA remained unchanged upon loss of POT1, …
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Characterization and Role of Secretogranin-II/Secretoneurin in Zebrafish Reproduction
… and secretogranin-IIb genes using TALENs in zebrafish indicates that these genes are required for optimal reproduction. Rates of oviposition for double secretogranin-II knockout females are 6% and 11% when crossed with double secretogranin-II knockout and wild-type males, …
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Characterization of the Placenta-Specific 8.1 Gene Function during Zebrafish Embryogenesis
… Transcription Activator-Like Effector Nucleases: TALENs), a method that utilizes sequence-specific nucleases. Similar to plac8.1 morphants, plac8.1stl33 embryos showed motile cilia morphology and beating function defects. We hypothesize that zebrafish Plac8.1 functions at the ciliary base, …
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Modeling Alzheimer's Disease in Induced Pluripotent Stem Cells
… in familial AD (FAD) PSEN1 mutants. Using TALENs, we generated a transgenic donor iPSC line that harbors a transgene for inducing the expression of fluorescently tagged tau protein and a recipient iPSC line that expresses membrane anchored YFP. These cell lines, when differentiated into …
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Single molecule investigation of transcription activator-like effector search dynamics
… yielding TALEN systems. Despite pervasive use of TALENs for editing crops, small animals, eukaryotic stem cells, and human T-cells, remarkably little is known about the molecular mechanisms used to locate and bind their DNA target sites. This work describes the application of single molecule …
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Engineering programmable nucleases for in vivo and in vitro applications
… transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR) associated nucleases, and Argonaute nucleases (Ago) have revolutionized genome engineering by allowing precise and easy manipulation of genetic material. These …
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Mammalian genome engineering: Fundamental investigation and method development
… correlates with their editing efficiency as TALENs are upto 5-times better than Cas9 in editing target-sites present in compact heterochromatin loci. These findings will govern design rules for developing genome-editing tools for rewiring heterochromatin as well as for biomedical …
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