Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"TALE nucleases"”.
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Cellular reprogramming in eukaryotes
… based on transcription activator-like effector (TALE) nucleases (TALENs), which allows researchers to design the DNA binding sequence through a simple cipher. In one example, we will discuss the development of a liquid phase high-throughput TALE synthesis platform (fairyTALE) capable of producing …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… transcription activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease. …
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Study and engineering of nucleic acid-binding repeat proteins
… transcription activator-like effectors (TALE) and RNA-binding Pumilio/fem-3 binding factor homology (PUF) proteins were shown to bind their target sequences in a single repeat/base fashion. This modular recognition nature allowed researchers to rationally design these proteins for novel …
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Gene editing in Aedes aegypti
… gene drive system in this mosquito. Homing endonucleases (HEs) are selfish elements which catalyze double-stranded DNA (dsDNA) breaks in a sequence-specific manner. The activities of four HEs (Y2-I-AniI, I-CreI, I-PpoI, and I-SceI) were investigated for their ability to catalyze the excision of …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… or a transcription activator-like effector (TALE) domain, is conjugated to a dimeric nuclease domain, producing zinc finger nucleases (ZFN) or TALE-nucleases (TALEN), respectively. By targeting these pairs of engineered nucleases adjacent to a site of interest, a DNA double-strand break (DSB) …
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Genetic Correction of Duchenne Muscular Dystrophy using Engineered Nucleases
… the native dystrophin gene using engineered nucleases that target one or more exons in a mutational hotspot in exons 45-55 of the dystrophin gene. Importantly, this hotspot mutational region collectively represents approximately 62% of all DMD mutations. In this work, we utilize various …