Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"TAL effector"”.
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Exploiting rice diversity to uncover durable and broad-spectrum resistance
… detection for quantitative trait loci (QTL). A total of 14 disease resistance QTL effective against multiple X. oryzae strains were mapped, 11 confer resistance to both pathovars, i.e. broad-spectrum resistance (BSR), and three are pathovar-specific. We also detected specific alleles conferring …
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… gene. To this end, we developed several pairs of TAL effector nucleases (TALENs) designed to target exon 1 of ADA. These TALENs function as dimers, and each pair creates a different targeted double strand break near the start site of the ADA gene. The most effective pair induces a DNA double …
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A versatile protein platform for sequence specific nucleic acid detection
… quantification of genetic material is fundamental for many research and diagnostic applications. Many different methods has been developed towards this objective, where each is typically tailored to detect a specific types of nucleic acid target under defined conditions. We have devised a …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… and pathology, and summarize the experimental techniques used for studies of oomycete genetics over the past two decades. Because the approach to studying fungi and oomycetes are similar (indeed they were incorrectly placed in the same taxonomic group until recently), a special section …
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AN INTEGRATED APPROACH TO THE DEVELOPMENT OF RESISTANCE TO COMMON BACTERIAL BLIGHT IN COMMON BEAN (PHASEOLUS VULGARIS)
… with different virulence features. Four Italian isolates causing CBB were screened on 2 common bean genotypes (one resistant and one susceptible). The screening underlined virulence differences in the susceptible genotype infection, but most importantly two Xpp isolates with contrasting …
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Cellular reprogramming in eukaryotes
… works is based on transcription activator-like effector (TALE) nucleases (TALENs), which allows researchers to design the DNA binding sequence through a simple cipher. In one example, we will discuss the development of a liquid phase high-throughput TALE synthesis platform (fairyTALE) capable of …
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Single molecule investigation of transcription activator-like effector search dynamics
… gene engineering. Transcription activator-like effectors (TALEs) are one such class that form a powerful gene-editing platform when fused to nuclease domains, thereby yielding TALEN systems. Despite pervasive use of TALENs for editing crops, small animals, eukaryotic stem cells, and human …