Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 5 of 5 for “"TAIL-PCR"”.
-
Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… Ds only plants was confirmed through multiplex PCR and the location of insertions within the genome was determined through TAIL-PCR. Resulting product sequences were blasted against the pre-publication tomato genome browser to determine insertion sites. A population of 309 independent …
-
Identifying genes related to symbiotic nitrogen fixation in Medicago truncatula Tnt1 insertion mutants using a forward genetics approach
… other two methods, thermal asymmetric interlaced PCR (TAIL-PCR) and whole genome sequencing (WGS), found a similar number of insertions (40 and 41, respectively). It was found that 50% of the insertions were located in coding regions. One of the Tnt1 insertions located on chromosome 5 is found …
-
Characterization of T-DNA integration sites within a population of insertional mutants of the diploid strawberry Fragaria vesca L.
… first we identified 11 homozygous lines by PCR, then another 55 homozygous lines by absence of segregation of GFP expression in T2 seedlings, and finally we attempted to distinguish homozygous from hemizygous lines by relative GFP expression measured using a commercially available GFP meter. …
-
Identification and Characterization of Genes Involved in Regulation of Ascorbate Metabolic Pathway(s) in Arabidopsis thaliana
… (wt) plants. Developmental studies, using RT-PCR, indicated that foliar expression of the VCF1 gene increased with plant age from 1 to 5 weeks, whereas AsA decreased during this same period. The expression of VCF1 was higher under a low-light condition in which AsA was reduced considerably. …
-
Genetic Transformation of Switchgrass (Panicum Virgatum L.) with Endoglucanase Gene and Characterization of Plants with Endoglucanase Transgene
… on the selection agent hygromycin, GUS assay, PCR amplification, southern blotting hybridization, for the presence of hygromycin and endoglucanase genes. However, based on RT-PCR analysis, only two transgenic lines were confirmed to produce mRNAs of the endoglucanase gene. These two transgenic …