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Showing 1 to 5 of 5 for “"T7 bacteriophage"”.

  1. Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide

    … the intergenic region of the single stranded DNA bacteriophage f1 and either a bacteriophage SP6 or T7 promoter have been constructed. Coinfection of E. coli with these chimeric plasmids and a single stranded DNA helper phage results in the replication and secretion of the pSP6(.)f1 or pTZ …

    uiuc Repository record for Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide (opens in a new tab)

  2. NON-FOULING AFFINITY PLATFORMS FOR PROTEIN IMMOBILIZATION IN ELECTRON MICROSCOPY

    … The selective and reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins has been observed. Our data illustrates the …

    purdue-thes Repository record for NON-FOULING AFFINITY PLATFORMS FOR PROTEIN IMMOBILIZATION IN ELECTRON MICROSCOPY (opens in a new tab)

  3. Synthesis and performance of novel supramolecular tools for single-particle cryogenic electron microscopy and drug and gene delivery

    … lipid coatings were then used to capture His6-T7 bacteriophage and His6-RplL from cell lysates, purified His8-GFPuv, and nanodisc embedded His6-MalFGK2. Graphene-oxide-NTA-based affinity grids were fabricated via covalent modification of graphene-oxide and monolayer fabrication on a Langmuir …

    purdue-thes Repository record for Synthesis and performance of novel supramolecular tools for single-particle cryogenic electron microscopy and drug and gene delivery (opens in a new tab)

  4. Expression Of Gal/galnac Lectin Of Entamoeba Histolytica In Transgenic Chloroplasts To Develop A Vaccine For Amebiasis

    … selection and gene10 regulatory sequence from T7 bacteriophage to enhance translation. The chloroplast integration of LecA was confirmed by PCR and Southern blot analysis. The expression of LecA protein in transgenic chloroplasts was analyzed by immunoblot analysis using anti-LecA antibodies. …

    ucf