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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 104 for “"T7"”.
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Simulation, models, and refactoring of bacteriophage T7 gene expression
… models for gene expression during bacteriophage T7 infection. T7 gene expression is a particularly well suited model system because knowledge of how the phage functions is thought to be relatively complete. My work focuses on two questions in particular. First, can we address deficiencies in past …
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The molecular basis of nucleotide recognition for T7 DNA polymerase
… is still under debate. An important study of T7 DNA polymerase performed by Tsai and Johnson using a conformationally sensitive fluorophore (CSF) characterized a conformational change directly and presented a new paradigm for nucleotide selectivity. This thesis describes work to further …
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Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators
… Fourteen terminator variants were analyzed by T7 RNA polymerase and we find that not only the hairpin structure itself but also its sequence influences termination. Furthermore, the formation of a hairpin in the RNA encoded by the A+T-rich regions of the attenuator is not mandatory for …
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
… the degradation of mRNA, and the maturation of T7 phage polycistronic early, middle, and late mRNAs. Bacteriophage T7 expresses a protein kinase (T7 PK), which is cAMP-independent, serine/threonine-specific, and phosphorylates over 90 cellular proteins, including RNase III during infection. …
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Density gradient studies of some T3 and T7 bacteriophages and their antibody complexes
Digitized by Kansas State University Libraries
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Density gradient studies of some T3 and T7 bacteriophages and their antibody complexes
Digitized by Kansas State University Libraries
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Grain Boundary Character Distribution in the HAZ of Friction Stir-Processed Al 7075 T7
… zone (HAZ) of friction stir welded Al 7075 T7 shows diminished corrosion resistance properties when compared with the parent material. Corrosion attack in this region of the weld is primarily intergranular and is associated with the presence of precipitate free zones. Current TEM research …
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Die Eigenschaften der Translationsinitiationsregion in E.coli: Effizienz verschiedener Signale aus dem Genom des Bakteriophagen T7
… Gene aus dem Genom des Bakteriophagen T7 weisen alle kurze Shine-Dalgarno (SD) Sequenzen (SD) sowie Unstrukturierte Regionen (USR) mit vorangehendem 5’-hairpin auf. Kurze TIR-Fragmente, die die SD-Sequenz beinhalten, vermittelten nur eine geringe Translationsaktivtät . Durch …
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Engineering the interface between cellular chassis and synthetic biological systems
… construction of cellular chassis expressing both T7 RNA polymerases (RNAP) and orthogonal ribosomes that are unused by the chassis but are available for use by an engineered system. I implement a network in which the orthogonal ribosomal RNA and the gene encoding T7 RNAP are transcribed by T7 …
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Gene Expression Control for Synthetic Patterning of Bacterial Populations and Plants
… systems, I explore the use of bacteriophage T7 RNA polymerase as a gene expression engine to control synthetic patterning across populations of cells. In E. coli, I developed a ratiometric assay of bacteriophage T7 RNA polymerase activity, which I used to systematically characterize different …
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Control Method for Invasive Aquatic Species introduced via Ballast Water: Effects of Carbon Dioxide Supersaturation on Survivorship of Digesia tigrina (Planaria: Maculata) and Lirceus brachyurus (Isopoda: Crustacea) and Effect of High Hydrostatic Pressure Processing on Freely Suspended and Shellfish Associated T7 Bacteriophage
… on Freely Suspended and Shellfish Associated T7 Bacteriophage The effectiveness of hydrostatic pressure processing (HPP) for inactivating viruses has only been evaluated in a limited number of studies and most of the work has been performed with freely suspended viruses. In this work, the …
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Single molecule fluorescence studies of replicative helicases
… recombination and repair. Bacteriophage T7 gp4 serves as one of the model proteins for replicative helicases. Moreover, the replication machinery of Bacteriophage T7 provides a simple model to study the dynamics of DNA replication. To address how the leading and lagging strand synthesis …
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The effect of passive and electronic amplitude-sensitive hearing protectors on the detection of a warning signal
… represented by the EAR Ultra 9000 and the Peltor T7-SR, respectively. Directly comparable earmuffs, the EAR 2000 and Peltor H7A, were used as conventional controls. The effect of the Peltor T7-SR’s gain on noise exposure and the attenuation of all earmuffs was also assessed. Signal detection …
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Environmental pollution and the cattle feedlot industry in the upper neosho watershed of Kansas
Call number: LD2668 .T4 1969 T7
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Environmental pollution and the cattle feedlot industry in the upper neosho watershed of Kansas
Call number: LD2668 .T4 1969 T7
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Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
… f1 and either a bacteriophage SP6 or T7 promoter have been constructed. Coinfection of E. coli with these chimeric plasmids and a single stranded DNA helper phage results in the replication and secretion of the pSP6(.)f1 or pTZ plasmids as single stranded DNA viral particles. A single …
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Bistability, Synthetic Biology, and Antibiotic Treatment
… point by a simple circuit consisting of mutant T7 RNA polymerase (T7 RNAP*) that activates its own expression in bacterium Escherichia coli. Although activation by the T7 RNAP* is noncooperative, the circuit caused bistable gene expression. This counterintuitive observation can be explained by …
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Identification of Protein-Phosphate Contacts in the R17 Coat Protein Binding Site
… sequence can be synthesized enzymatically using T7 RNA polymerase and synthetic DNA templates which contain the T7 promoter. The templates are base paired from $-17$ to +1 and contain a long 5$\sp\prime$ overhang. The products of the transcription reactions have the length and sequence expected …
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Vectores de expresión de cDNAs genómicos de Rabbit vesivirus (RaV) y análisis de su funcionalidad como clones infectivos
… de las RNA-polimerasas de los fagos SP6 y T7. Estos vectores se usaron en ensayos de transcripción in vitro y el RNA de longitud genómica así obtenido se empleó para intentar el rescate de virus mediante transfección de líneas celulares de mamíferos de distintos orígenes (Hek 293T, Vero, …
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